Antigen Unmasking in Formalin-Fixed Routinely Processed Paraffin Wax-Embedded Sections by Pressure Cooking

Miller, KD; Auld, John; Jessup, Eileen; Rhodes, Anthony; Ashton‐Key, Margaret

doi:10.1097/00125480-199501000-00044

Cited by 68 publications

(23 citation statements)

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“…To enhance the immunostaining, a heat epitope retrieval procedure was performed using a Black-and-Decker vegetable steamer (Shelton, CT, USA) as previously described. 25 The antigen-antibody immunoreaction was visualized using either 3-amino-9-ethylcarbazole or 3,3 0 -diaminobenzidine as chromogen. To evaluate the specificity of the immunoreaction, known positive and negative tissues were used as controls.…”

Section: Methodsmentioning

confidence: 99%

Deciduoid mesothelioma: report of 21 cases with review of the literature

Ordóñez

2012

Modern Pathology

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Deciduoid mesothelioma is a rare variant of epithelioid mesothelioma that was initially considered to occur exclusively in the peritoneum of young women who had no history of asbestos exposure and to be characterized by an aggressive clinical course, but it was later demonstrated that this tumor could also occur in the pleura of older men and women who had been exposed to asbestos. Some subsequent studies have also indicated that the clinical course is no different from that of conventional epithelioid mesothelioma. Herein are reported 21 cases of deciduoid mesothelioma that were investigated using a large panel of immunohistochemical markers, 9 of which were also studied by electron microscopy. Fifteen of the patients were male and 6 were female (mean age, 60 years). Seventeen of the cases originated in the pleura and four in the peritoneum. Histologically, all of the cases were composed of large, polygonal or ovoid cells with well-defined cell borders, dense eosinophilic cytoplasm, and single or multiple nuclei. In some cases, the cells exhibited a wide variation in their size and shape, frequent loss of cell cohesion, marked nuclear atypia, and high mitotic activity (45 per 10 HPF); whereas, in others, the cells were more cohesive, less pleomorphic, and the mitotic activity low. As the survival of patients in the first group of cases was shorter (mean, 7 months), when compared with that of the latter (mean, 23 months), it is concluded that the differences in prognosis reported in deciduoid mesothelioma are due to the existence of a high-grade subgroup that presents highly aggressive clinical behavior. Therefore, when a high-grade deciduoid mesothelioma is present, it should be reported as it can significantly affect prognosis and treatment. The use of immunohistochemistry and electron microscopy in assisting in the differential diagnosis of deciduoid mesothelioma is also discussed.

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Section: Methodsmentioning

confidence: 99%

Deciduoid mesothelioma: report of 21 cases with review of the literature

Ordóñez

2012

Modern Pathology

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“…To enhance the immunostaining, a heat epitope retrieval procedure was performed using a Black-and-Decker vegetable steamer (Shelton, CT, USA) as previously described. 18 The antigen-antibody immunoreaction was visualized using either 3-amino-9-ethylcarbazole or 3,3 0 -diaminobenzidine as chromogen. To evaluate the specificity of the immunoreaction, known positive and negative tissues were used as controls.…”

Section: Methodsmentioning

confidence: 99%

Pleomorphic mesothelioma: report of 10 cases

Ordóñez

2012

Modern Pathology

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Mesotheliomas with pleomorphic features are rare and only a few studies on this mesothelioma variant have been published. Little information regarding the immunoprofile of these tumors and none on their electron microscopic features was included in these studies. Herein are reported 10 cases of pleomorphic mesothelioma that were investigated using a large panel of immunohistochemical markers, 4 of which were also studied by electron microscopy. All of the patients were men and seven had a history of asbestos exposure. Nine of the cases originated in the pleura and one in the peritoneum. Histologically, the tumors were characterized by being composed of large, often discohesive, cells that varied in size and shape, had dense abundant eosinophilic cytoplasm, and single or multiple irregular nuclei, which often contained one or several large nucleoli. Mitotic activity was high and atypical mitoses frequent. Immunoreactivity for pan-keratin and keratin 7 was strong in all of the cases. Expression for calretinin, WT1, podoplanin, mesothelin and keratin 5/6 was also frequent, but variable. All cases were negative for MOC-31, carcinoembryonic antigen, CD15, TAG-72 and thyroid transcription factor-1. Electron microscopy often showed the presence of abundant long, slender microvilli on the cell membrane of the neoplastic cells. These findings demonstrate that, contrary to what has been suggested by some investigators, both immunohistochemistry and electron microscopy can be very helpful in assisting in the diagnosis of pleomorphic mesotheliomas. That the seven patients who underwent extrapleural pneumonectomy had extensive lymph node metastasis and that the median survival of those patients for whom follow-up information was available was only 8.2 months indicates that mesotheliomas with pleomorphic features are associated with highly aggressive clinical behavior. Therefore, when this subtype of epithelioid mesothelioma is present, it should be reported as it can significantly affect the prognosis and treatment of the patient.

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“…This required a special procedure, with the use of antigen-exploiting solution (of high pH value, containing NaOH) recommended for decalcified tissues. The method is described in detail elsewhere [20,24].…”

Section: Methodsmentioning

confidence: 99%

The prognostic significance of DNA cytophotometry and proliferation index (Ki-67) in giant cell tumors of bone

Antal

Szepes²,

Szabó

1999

International Orthopaedics

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We studied DNA ploidy by smear cytophotometry and proliferation activity by Ki-67 MIB immunohistochemistry in 69 primary and recurrent giant cell tumors (GCT) from 50 randomly selected patients. The obtained results were evaluated with comparisons made to the available clinical data. From the 46 primary tumors 63% showed diploidy and 37% aneuploidy. A significantly (P=0.026) higher recurrence rate (64%) was observed in aneuploid than in diploid tumors (31%). In the course of the recurrences, both the ratio of aneuploid tumors as well as the proliferation index of the tumors increased, though the degree of the latter was non-significant. Aneuploidy did not mean an unambiguous tendency towards malignant transformation; however, a close follow-up of recurrent aneuploid tumors, and wide excision of the recurrence instead of intralesional curettage are the recommended procedures. The DNA cytophotometry and proliferation index of GCTs -as compared to other histologic examinations -are of prognostic value in the evaluation of the recurrence potential of the GCTs.Résumé Les auteurs ont examiné 1'anomalie chromosomique de DNA á 1'aide de cytophotometrie de frottis; et 1'activité de proliferation par le moyen de 1'immunohistochimie Ki-67 MIB dans 69 tumeurs de cellules gé-antes de 50 patients choisis accidentellement. lls ont éva-lué les résultats en les comparant aux données cliniques disponibles. Parmi les 46 tumeurs primaires 63% ont manifesté un caractère diploide et 37% un caractère aneuploide. On a observé une proportion de recidive significativement plus élevée (P=0.026) dans les tumeurs aneuploides (64%) que dans les tumeurs diploides (31%).Au cours des récidives on a remarquè une augmentation tant dans la proportion des tumeurs aneuploides que dans 1'indice de proliferation mais en ce dernier 1'augmentation n'était pas significative. Le caractère aneuploide ne signifie pas forcément une tendance univoque á la transformation maligne cependant dans les tumeurs aneuploides récidivantes il est nécessaire de suivre la maladie avec beaucoup de soin et comme intervention chirurgicale on conseille une excision large au lieu du curettage intralésionel.

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Antigen Unmasking in Formalin-Fixed Routinely Processed Paraffin Wax-Embedded Sections by Pressure Cooking

Cited by 68 publications

References 0 publications

Deciduoid mesothelioma: report of 21 cases with review of the literature

Deciduoid mesothelioma: report of 21 cases with review of the literature

Pleomorphic mesothelioma: report of 10 cases

The prognostic significance of DNA cytophotometry and proliferation index (Ki-67) in giant cell tumors of bone

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