Antigen-Specific Synergism in the Immune Response of Irradiated Mice Given Marrow Cells and Peritoneal Cavity Cells or Extracts

Kennedy, James C.; Treadwell, Perry E.; Lennox, Edwin S.

doi:10.1084/jem.132.2.353

Cited by 24 publications

(9 citation statements)

References 22 publications

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“…Although thymocytes appear to be antigen-specific (3,7,9,22,23), and do not generate antibody-forming cells (42,43), whereas marrow elements are the precursors of plaque-forming cells (31), it cannot be excluded that bone marrow cells also play an active role in recognition. In fact, results of recent studies indicate the presence of specific precursors in bone marrow (9)(10)(11)34). Since marrow contains cells possessing the properties of recognition and of antibody production, it is not possible from the data presented here to determine whether the immunological genetic defect for (Phe,G)-Pro-L is one of antigenic recognition, of antibody production, or of both.…”

Section: Discussionmentioning

confidence: 38%

Contribution of Bone Marrow Cells and Lack of Expression of Thymocytes in Genetic Controls of Immune Responses for Two Immunopotent Regions Within Poly-(Phe,glu)-Poly-Pro--Poly-Lys in Inbred Mouse Strains

Mozes

Shearer

1971

The Journal of Experimental Medicine

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Previous cellular studies on the genetic regulation of immunological responsiveness for two immunopotent regions within the branched chain synthetic polypeptide (Phe, G)-Pro--L demonstrated a direct correlation between the number of detectable immunocompetent splenic precursor cells and the response patterns of SJL, DBA/1, and F1 mice (21). In order to establish the cellular origin(s) of the genetic defect, the present study first demonstrated that thymus and bone marrow cell cooperation was required for (Phe, G)- and Pro--L-specific immune responses. Secondly, limiting dilution experiments, in which several graded and limiting inocula of marrow cells were mixed with a non-limiting number of 108 thymocytes and injected into irradiated, syngeneic recipients, indicated that the low responsiveness of the SJL and DBA/1 strains to the (Phe, G) and Pro--L specificities, respectively, could be attributed to a reduced number of precursor cells found in bone marrow. About five times more marrow precursors were detected in SJL mice for Pro--L than for (Phe, G), whereas about five times as many precursor cells were estimated for (Phe, G) as for Pro--L in the DBA/1 strain. These differences are similar to those obtained using spleen cells from unimmunized SJL and DBA/1 donors (21), and indicate that these genetically determined variations in responsiveness can be accounted for by differences in the frequencies of monospecific populations of immunocompetent cells present in bone marrow. In contrast, limiting dilution transfers of thymocytes or thymus-derived cells with an excess of syngeneic marrow cells resulted in equally frequent (Phe, G) and Pro--L responses for both SJL ad DBA/1 strains. This finding in conjunction with the observation that the generation of (Phe, G)- and Pro--L-specific responses were associated in individual recipients injected with limiting inocula of thymocytes indicated that a single population of thymocytes was stimulated by (Phe,G)-Pro--L. Therefore, it is improbable that the thymic population of immunocompetent cells contributes to expression of these genetically controlled defects.

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Section: Discussionmentioning

confidence: 38%

Contribution of Bone Marrow Cells and Lack of Expression of Thymocytes in Genetic Controls of Immune Responses for Two Immunopotent Regions Within Poly-(Phe,glu)-Poly-Pro--Poly-Lys in Inbred Mouse Strains

Mozes

Shearer

1971

The Journal of Experimental Medicine

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“…The results presented here and elsewhere (13)(14)(15)(16)18) strongly suggest that bone marrow-derived cells possess all the information necessary to dictate the specificity of the antibody response without help by other cell types. The demonstration that the number and immunocompetence of marrow P-PFC increase specifically upon immunization (reference 15 and Table I) and that the more efficient P-PFC* follow a different pattern of proliferation and differentiation than virgin precursor cells suggests that the immunologic memory of mice to SRBC rests primarily on marrow-derived cells.…”

Section: Discussionmentioning

confidence: 70%

Immunologic Memory Cells of Bone Marrow Origin

Miller

Cudkowicz

1972

The Journal of Experimental Medicine

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Individual immunocompetent precursor cells of (C57BL/10 x C3H)F1 mouse marrow generate, on transplantation, three to five times more antibody-forming cells localized in recipient spleens during secondary than during primary immune responses. The increased burst size is immunologically specific since antigens of horse and chicken erythrocytes and of Salmonella typhimurium do not cause this effect in marrow cells responsive to sheep red blood cells. Both sensitized and nonsensitized precursors require the helper function of thymus-derived cells and antigen for the final steps of differentiation and maturation. The burst size of primed precursor cells is the same after cooperative interactions with virgin or educated helper cells of thymic origin. The greater potential of these marrow precursors may be attributable to self-replication and migration before differentiation into antibody-forming descendants. In fact, the progeny cells of primed precursor units are distributed among a multiplicity of foci, whereas those of nonimmune precursors are clustered into one focus. The described properties of specifically primed marrow precursors are those underlying immunologic memory. It remains to be established whether memory cells are induced or selected by antigens and whether the thymus plays a role in this process.

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“…Recent reports in the literature have lent support to this hypothesis (Kennedy, Treadwell, and Lennox, 1970;Basten 1972a, 1972b). Kennedy, Treadwell and Lennox (1970) observed synergism in anti-erythrocyte responses between bone marrow and peritoneal cavity cells when both cell types were used to reconstitute irradiated recipients. They noted that heat-killed peritoneal cells or extracts of these cells were also effective.…”

Section: Transfer Of Lymphoid Cells From Bovine Gamma Globulin (Bgg)mentioning

confidence: 68%

Comparison of the ease of tolerance induction in thymus and bone marrow of three strains of mice

Blackstock

Hyde

1973

Cellular Immunology

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Antigen-Specific Synergism in the Immune Response of Irradiated Mice Given Marrow Cells and Peritoneal Cavity Cells or Extracts

Cited by 24 publications

References 22 publications

Contribution of Bone Marrow Cells and Lack of Expression of Thymocytes in Genetic Controls of Immune Responses for Two Immunopotent Regions Within Poly-(Phe,glu)-Poly-Pro--Poly-Lys in Inbred Mouse Strains

Contribution of Bone Marrow Cells and Lack of Expression of Thymocytes in Genetic Controls of Immune Responses for Two Immunopotent Regions Within Poly-(Phe,glu)-Poly-Pro--Poly-Lys in Inbred Mouse Strains

Immunologic Memory Cells of Bone Marrow Origin

Comparison of the ease of tolerance induction in thymus and bone marrow of three strains of mice

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