Antigen-specific directional target cell lysis by perforin-negative T lymphocyte clones

Takayama, Hajime; Shinohara, Nobukata; Kawasaki, Akemi; Someya, Yoshiko; Hanaoka, Satoko; Kojima, Hiroko; Yagita, Hideo; Okumura, Ko; Shinkai, Yoichi

doi:10.1093/intimm/3.11.1149

Cited by 32 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…44). We also have reported that several class II-restricted soluble antigen-specific murine CD4+ T-cell clones, including BK1 (used in the present study), could efficiently lyse the cognate antigen-presenting cells without a detectable level ofperforin (8). The target cell lysis exerted by these clones was associated with target cell DNA fragmentation (one of the characteristics of apopototic cell death) and was contactdependent and TNF-independent.…”

supporting

confidence: 56%

Fas and its ligand in a general mechanism of T-cell-mediated cytotoxicity.

Hanabuchi

Koyanagi

Kawasaki

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

260

115

View full text Add to dashboard Cite

show abstract

supporting

confidence: 56%

Fas and its ligand in a general mechanism of T-cell-mediated cytotoxicity.

Hanabuchi

Koyanagi

Kawasaki

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

260

115

View full text Add to dashboard Cite

show abstract

“…Mouse CD8ϩ CTL clone OE4 (anti-H-2 d ) (18) was stimulated every 4 weeks with irradiated splenocytes from DBA/2 mice in EL4-conditioned medium, expanded 4 days after stimulation, and harvested at day 8 (4 days after expansion) (19). Mouse B cell lymphoma line A20.2J (H-2 d ) and mouse T cell leukemia line EL4 (H-2 b ) were used as specific and nonspecific target cells for OE4, respectively.…”

Section: Methodsmentioning

confidence: 99%

Memory of Extracellular Adenosine A2A Purinergic Receptor-mediated Signaling in Murine T Cells

Koshiba

Kojima

Huang

et al. 1997

Journal of Biological Chemistry

Self Cite

173

View full text Add to dashboard Cite

“…ϩ CTL clone, and anti-H2d CTL clone GD1 (FasL deficient, derived from gld mice) were maintained according to published procedures (18,19).…”

Section: Cells and Mediummentioning

confidence: 99%

Differential Effects of Physiologically Relevant Hypoxic Conditions on T Lymphocyte Development and Effector Functions

Caldwell

Kojima

Lukashev

et al. 2001

The Journal of Immunology

Self Cite

362

319

View full text Add to dashboard Cite

Direct measurements revealed low oxygen tensions (0.5–4.5% oxygen) in murine lymphoid organs in vivo. To test whether adaptation to changes in oxygen tension may have an effect on lymphocyte functions, T cell differentiation and functions at varying oxygen tensions were studied. These studies show: 1) differentiated CTL deliver Fas ligand- and perforin-dependent lethal hit equally well at all redox conditions; 2) CTL development is delayed at 2.5% oxygen as compared with 20% oxygen. Remarkably, development of CTL at 2.5% oxygen is more sustained and the CTL much more lytic; and 3) hypoxic exposure and TCR-mediated activation are additive in enhancing levels of hypoxia response element-containing gene products in lymphocyte supernatants. In contrast, hypoxia inhibited the accumulation of nonhypoxia response element-containing gene products (e.g., IL-2 and IFN-γ) in the same cultures. This suggests that T cell activation in hypoxic conditions in vivo may lead to different patterns of lymphokine secretion and accumulation of cytokines (e.g., vascular endothelial growth factor) affecting endothelial cells and vascular permeabilization. Thus, although higher numbers of cells survive and are activated during 20% oxygen incubation in vitro, the CTL which develop at 2.5% oxygen are more lytic with higher levels of activation markers. It is concluded that the ambient 20% oxygen tension (plus 2-ME) is remarkably well suited for immunologic specificity and cytotoxicity studies, but oxygen dependence should be taken into account during the design and interpretation of results of in vitro T cell development assays and gene expression studies in vivo.

show abstract

Antigen-specific directional target cell lysis by perforin-negative T lymphocyte clones

Cited by 32 publications

References 0 publications

Fas and its ligand in a general mechanism of T-cell-mediated cytotoxicity.

Fas and its ligand in a general mechanism of T-cell-mediated cytotoxicity.

Memory of Extracellular Adenosine A2A Purinergic Receptor-mediated Signaling in Murine T Cells

Differential Effects of Physiologically Relevant Hypoxic Conditions on T Lymphocyte Development and Effector Functions

Contact Info

Product

Resources

About