Antigen shedding by human breast-cancer cells in vitro and in vivo

Nordquist, RE; Anglin, J. H.; Lerner, Michael

doi:10.1038/bjc.1978.115

Cited by 14 publications

(3 citation statements)

References 6 publications

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“…In parallel with this, we have previously reported that NSCLC tumors express significant levels of the immunomodulatory cytokines, IL-1 receptor antagonist and IL-10 [38,39]. This phenomenon, combined with the shedding of soluble tumor-speci®c antigens [2,10,30,31] and reduced cell-surface expression of class I MHC antigens [9,43], may provide the tumor with a defense against immune surveillance.…”

Section: Discussionmentioning

confidence: 81%

Macrophage infiltration in human non-small-cell lung cancer: the role of CC chemokines

Arenberg¹,

Keane²,

DiGiovine

et al. 2000

Cancer Immunology, Immunotherapy

130

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Bronchogenic carcinoma is the leading cause of malignancy-related mortality in the United States, with an overall 5-year survival rate of less than 15%. This aggressive behavior reflects, among other traits, the capacity of the tumor to evade normal host immune defenses, and to induce a pro-angiogenic environment. A central feature of any immune response toward tumors is the recruitment of specific immune cell populations. In the present study we investigated the infiltration of monocytes in human specimens of non-small-cell lung cancer (NSCLC). The presence of macrophages in NSCLC tumors was documented by immunohistochemistry. In vitro chemotaxis assays demonstrated higher monocyte chemotactic activity in NSCLC tumor homogenates than in normal lung tissue. We next investigated the expression of CC chemokines within specimens of NSCLC tumors. Levels of the CC chemokines were higher in NSCLC tumor tissue than in normal lung tissue. Immunolocalization showed that the cells associated with antigenic CC chemokines were the malignant tumor cells, as well as occasional stromal cells. Maximal inhibition of monocyte chemotaxis induced by NSCLC in vitro occurred in the presence of neutralizing antibodies to MCP-1 and MIP-1beta. On follow-up of 15 patients in whom we quantified macrophage infiltration, we found that those with recurrence of disease had higher levels of macrophage infiltration in their initial tumors. However, the functional significance of CC-chemokine-mediated macrophage infiltration into NSCLC remains to be determined.

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Section: Discussionmentioning

confidence: 81%

Macrophage infiltration in human non-small-cell lung cancer: the role of CC chemokines

Arenberg¹,

Keane²,

DiGiovine

et al. 2000

Cancer Immunology, Immunotherapy

130

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“…that the rate of turnover of tumor cell membrane antigens is related to the biological behavior of the tumor in vivo, particularly its immunogenicity and capacity for metastasis [12,13]. Tumor cells with metastatic capacities have been shown to shed glycoproteins from the cell surface into the host circulation [3,14] and to possess a greatly accelerated glycoprotein metabolism [4].…”

Section: Rat Hepatic (H) and Tumor (T) Cells The Cell Surface Of Thementioning

confidence: 99%

Ultrastructural study of membrane glycocalyx in primary and metastatic human and rat mammary carcinoma

Ghosh

Nassauer

Faiferman

et al. 1981

Journal of Surgical Oncology

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The glycocalyx of primary and metastatic tumors was compared by ruthenium red staining and electron microscopy. Two systems were studied: human primary and metastatic breast tumors and rat primary and metastatic mammary carcinomas. A considerable diminution in the amount of glycocalyx was detected in both systems when primary tumors were compared with metastases. These results are consistent with observations of others showing that metastatic tumors shed glycoprotein antigens to the circulation; this may provide an immune escape mechanism for tumor cells, allowing their dissemination.

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“…These antigens are glycoproteins or whole plasma membrane fragments (De Broe et al, 1977) which circulate in a free state or complex with host immunoglobulins (Sjogren et al, 1971;Bowen et al, 1975). Although a number of groups have demonstrated the release of human and animal cell-surface proteins into the serum of tumour-bearing animals (Jamasbi et al, 1978;Nordquist et al, 1978; Rao & Bonavida, 1977;Primus et al, 1976) a study of this process is complicated by the host's immune system.…”

mentioning

confidence: 99%

Production of antibodies against antigens released from human pancreatic tumour xenografts

Grant¹,

Duke²

1981

Br J Cancer

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Antibodies directed against the antigen released from viable tumour cells during growth have been raised by cross-immunization of immunocompetent hairy litter-mates with serum from nude mice bearing pancreatic tumour xenografts. Antisera raised against the components released from a primary pancreatic tumour xenograft (WB) or from a tumour cell-line xenograft (GER) showed a titre greater than 1:625 against cultured pancreatic tumour cells by an I125-binding assay. Five out of 14 of the hairy litter-mates immunized with serum from the same tumour (GER) produced antisera that bound more strongly to pancreatic cancer cells than to 13 other non-pancreatic cell lines (binding ratio greater than 2). Absorption of the antisera with pure CEA reduced the level of binding by 11-25% without affecting the specificity for pancreatic tumour cells. The antibody response could be completely removed by absorption with pancreatic tumour cells, whereas 50% and 18% of the activity remained after absorption with normal pancreas homogenate and a mixed non-pancreatic tumour-cell pool, respectively. Immunofluorescent staining of pancreatic tumour sections indicated that the antibody was localized on the membrane of ductular epithelial cells. Challenge of immunocompetent mice using this procedure has produced a polyspecific antiserum with signs of selectivity for the circulating antigens released from pancreatic cancer cells, and may provide a route to the production of antibody against specific tumour components.

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Antigen shedding by human breast-cancer cells in vitro and in vivo

Cited by 14 publications

References 6 publications

Macrophage infiltration in human non-small-cell lung cancer: the role of CC chemokines

Macrophage infiltration in human non-small-cell lung cancer: the role of CC chemokines

Ultrastructural study of membrane glycocalyx in primary and metastatic human and rat mammary carcinoma

Production of antibodies against antigens released from human pancreatic tumour xenografts

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