Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion

Conzentino, Marcelo dos Santos; Forchhammer, Karl; Souza, Emanuel Maltempi de; Pedrosa, Fábio O.; Nogueira, Meri Bordignon; Raboni, Sonia M; Rego, Fabiane Gomes de Moraes; Zanette, Dalila Lucíola; Aoki, Mateus Nóbrega; Nardin, Jeanine Marie; Fornazari, Bruna; Morales, Hugo Manuel Paz; Celedón, Paola Alejandra Fiorani; Lima, Carla V P; Mattar, S. B.; Lin, Vanessa H; Morello, Luis Gustavo; Marchini, Fabrício Klerynton; Reis, Rodrigo Arantes; Huergo, Luciano F.

doi:10.1007/s42770-021-00556-6

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…The details of antigen purification and bead preparation have been described previously (Alvim et al . 2020; Conzentino et al . 2021a).…”

Section: Metodologiamentioning

confidence: 99%

“…Samples were centrifuged (5,000 x g for 3 min) and four microliters serum was used to investigate IgG reactive against the SARS-CoV-2 Nucleocapsid or full prefusion Spike using a high throughput magnetic immunofluorescent assay described previously (Conzentino et al 2021b;Huergo et al 2021). The details of antigen purification and bead preparation have been described previously (Alvim et al 2020;Conzentino et al 2021a). The results of each sample were reported and the % of a reference and the method operated with specificity and sensitivity of >99.5% and >95% respectively (Conzentino et al 2021b) .…”

Section: Study Design and Samplingmentioning

confidence: 99%

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SARS-CoV-2 seroconversion in response to infection and vaccination: A time series local study in Brazil

Huergo¹,

Paula²,

Gonçalves³

et al. 2022

Preprint

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The investigation of antibodies raised against different SARS-CoV-2 antigens can help to determine the extent of previous SARS-CoV-2 infections in the population and track the humoral response to vaccination. Therefore, serological surveys can provide key information to better manage the pandemic and/or to implement the most effective vaccination program. Here we describe a time series anti-Nucleocapsid, anti-Spike IgG serological survey analysis in the city of Matinhos, PR, Brazil during the year of 2021. Seroconversion rates to the Nucleocapsid antigen was not influenced by gender or age. Comparison of the serological data with official COVID-19 cases in the city suggest that case sub notification is higher than 47%. Furthermore, by applying serological data, the corrected infection fatality rate was estimated to be lower than 2.4 % in contrast with the official estimative of 3.6 %. The rates of IgG reactive to Spike antigen resembled the curve of the fraction the population that had taken the second vaccine dose. Up to 82% of Spike seroconversion was detected in the end of 2021 confirming the effective of the COVID-19 vaccination program in the city. This SARS-CoV-2 serological study unraveled the SARS-CoV-2 infection rates and the response to vaccination in the city of Matinhos, it is likely that the numbers reported here may be similar in other cities in Brazil.

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“…The details of antigen purification and bead preparation have been described previously (Alvim et al . 2020; Conzentino et al . 2021a).…”

Section: Metodologiamentioning

confidence: 99%

Section: Study Design and Samplingmentioning

confidence: 99%

SARS-CoV-2 seroconversion in response to infection and vaccination: A time series local study in Brazil

Huergo¹,

Paula²,

Gonçalves³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Samples were centrifuged (5,000 × g for 3 min), and four microliters serum was used to investigate IgG reactive against the SARS-CoV-2 nucleocapsid or full prefusion spike using an in-house high-throughput magnetic immunofluorescent assay, which has been described previously ( 10 , 11 ). The details of antigen purification and bead preparation have been described previously ( 12 , 13 ). The results of each sample were reported and the percentage of a reference serum.…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 Seroconversion in Response to Infection and Vaccination: a Time Series Local Study in Brazil

et al. 2022

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“…All antigen sequences were based on the original Wuhan isolate. The details of antigen purification and bead preparation have been described previously (Conzentino et al . 2021a, 2021b, 2022; Huergo et al .…”

Section: Methodsmentioning

confidence: 99%

Populational analysis of the immunoglobulin G response to different COVID-19 vaccines in Brazil

Paula

Conzentino

Gonçalves

et al. 2022

Preprint

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Vaccination is a strategy that confers protection against symptomatic infections and/or development of severe COVID-19. In Brazil, COVID-19 vaccination began in January 2021 and has been performed using vaccines from different manufactures including CoronaVac (Sinovac), ChAdOx1 (Oxford/AstraZeneca) and BNT162b2 (Pfizer/BioNTech). One of the main protective mechanisms triggered by vaccination involves the production of IgG antibodies reactive to the Spike antigen of SARS-CoV-2, the levels of which correlates with vaccine efficacy. Although phase III clinical studies confirmed the efficacy of the vaccines used in Brazil, there are just few studies comparing vaccine immunogenicity in a real-world scenario. This study aimed to depict the IgG response to natural infections and to vaccination using different types of vaccines at population scale in Matinhos, a city located in south of Brazil. Nucleocapsid seroconversion rates indicated that more than a quarter of the cohort has been subjected to natural infections by SARS-CoV-2 by the first trimester of 2022. Spike seroconversion rates achieved >95% by February 2022 and maintained stable as far as June 2022 confirming the effectiveness of the vaccination program. Immunogenicity concerning IgG reactive to Spike was higher using the BNT162b2 vaccine, followed by ChAdOx1 and CoronaVac. Natural infections boosted IgG levels reactive to Spike in those individuals that completed primary vaccination with ChAdOx1 and CoronaVac but not with BNT162b2. The levels of IgG reactive to Spike increased with the number of vaccine doses administered. The application of BNT162b2 as booster dose resulted in high levels of IgG reactive to Spike which were similar despite the type of the vaccine used during primary vaccination.

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Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion

Cited by 7 publications

References 12 publications

SARS-CoV-2 seroconversion in response to infection and vaccination: A time series local study in Brazil

SARS-CoV-2 seroconversion in response to infection and vaccination: A time series local study in Brazil

SARS-CoV-2 Seroconversion in Response to Infection and Vaccination: a Time Series Local Study in Brazil

Populational analysis of the immunoglobulin G response to different COVID-19 vaccines in Brazil

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