Antigen presentation by mouse monocyte-derived cells: Re-evaluating the concept of monocyte-derived dendritic cells

Coillard, Alice; Ségura, Elodie

doi:10.1016/j.molimm.2021.04.012

Cited by 16 publications

(10 citation statements)

References 56 publications

(67 reference statements)

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“…This has formed the basis of many clinical trials in cancer patients ( Romero et al, 2016 ). Moreover, monocyte transfer in mice lacking cDCs has been able to restore key antigen-presenting functions, pointing to a clear potential of monocytes as APCs ( Coillard and Segura, 2021 ; Kool et al, 2008b ; Kool et al, 2011 ; Leal et al, 2021 ; Leon et al, 2007 ; Plantinga et al, 2013 ). Nevertheless, there have been some recent doubts showing that the protocols used to generate mouse moDCs, mainly generate macrophage-like cells and cDC2s ( Helft et al, 2015 ) and that the classical way by which moDCs were defined in vivo by flow cytometry, were essentially often a contamination of cDC2s and macrophages ( Bosteels et al, 2020b ).…”

Section: Resultsmentioning

confidence: 99%

“…Yet, we and others have reported that peripheral monocytes also capture and transport antigen to LNs ( Jakubzick et al, 2013 ; Jakubzick et al, 2017 ; Randolph et al, 1999 ), whereas these cells poorly express CCR7 on their surface. Antigen presentation by monocytes appears to be less controversial than their migratory capabilities since there are ample studies supporting monocytes as APCs when they were adoptively transferred ( Coillard and Segura, 2021 ; Kool et al, 2008a ; Kool et al, 2008b ; Kool et al, 2011 ; Leal et al, 2021 ; Leon et al, 2007 ; Plantinga et al, 2013 ). Moreover, their main route of entry into the LNs is thought to be through high endothelial venules, like resident cDCs.…”

Section: Introductionmentioning

confidence: 99%

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CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes

Rawat

Tewari

et al. 2023

Journal of Experimental Medicine

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Dendritic cells (DCs) and monocytes capture, transport, and present antigen to cognate T cells in the draining lymph nodes (LNs) in a CCR7-dependent manner. Since only migratory DCs express this chemokine receptor, it is unclear how monocytes reach the LN. In steady-state and following inhalation of several PAMPs, scRNA-seq identified LN mononuclear phagocytes as monocytes, resident, or migratory type 1 and type 2 conventional (c)DCs, despite the downregulation of Xcr1, Clec9a, H2-Ab1, Sirpa, and Clec10a transcripts on migratory cDCs. Migratory cDCs, however, upregulated Ccr7, Ccl17, Ccl22, and Ccl5. Migratory monocytes expressed Ccr5, a high-affinity receptor for Ccl5. Using two tracking methods, we observed that both CD88hiCD26lomonocytes and CD88−CD26hi cDCs captured inhaled antigens in the lung and migrated to LNs. Antigen exposure in mixed-chimeric Ccl5-, Ccr2-, Ccr5-, Ccr7-, and Batf3-deficient mice demonstrated that while antigen-bearing DCs use CCR7 to reach the LN, monocytes use CCR5 to follow CCL5-secreting migratory cDCs into the LN, where they regulate DC-mediated immunity.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes

Rawat

Tewari

et al. 2023

Journal of Experimental Medicine

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“…In this process, it does not matter whether a suitable gating strategy is available. For example, using the marker CD26 allowed us to distinguish cDC2 from monocyte‐derived DC and CD26 was suggested to be included in the minimal set of phenotypic markers to identify cDC, which appear as the CD26 + subset [70]. In summary, accuracy for the annotation of flow cytometry datasets can be achieved using FlowSOM clustering followed by “semisupervised” assignment of cell types to FlowSOM populations based on expression patterns identified using manual gating strategies ideally applied to the same dataset before.…”

Section: Discussionmentioning

confidence: 99%

Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues

et al. 2022

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This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues.DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single‐cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in‐depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues.While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer‐reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.

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“…While mo-DCs can be detected mainly in blood and tissues under physiological conditions, they are most frequently produced during inflammation and have a high degree of plasticity and variety in their roles [ 44 ]. These functions are greatly impacted by the microenvironment that is regulated and created by local DCs [ 45 ]. Human CD14 + /CD16 − monocytes perform context-dependent tasks, such as extravasating into tissues and differentiating into monocyte-derived macrophages, which produce IL-10 and encourage tolerance.…”

Section: Mo-dcsmentioning

confidence: 99%

Tumor-Infiltrating Dendritic Cells: Decisive Roles in Cancer Immunosurveillance, Immunoediting, and Tumor T Cell Tolerance

Katopodi

Petanidis

Charalampidis

et al. 2022

Cells

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The tumor microenvironment plays a key role in progression of tumorigenesis, tumor progression, and metastasis. Accumulating data reveal that dendritic cells (DCs) appear to play a key role in the development and progression of metastatic neoplasia by driving immune system dysfunction and establishing immunosuppression, which is vital for tumor evasion of host immune response. Consequently, in this review, we will discuss the function of tumor-infiltrating DCs in immune cell signaling pathways that lead to treatment resistance, tumor recurrence, and immunosuppression. We will also review DC metabolism, differentiation, and plasticity, which are essential for metastasis and the development of lung tumors. Furthermore, we will take into account the interaction between myeloid cells and DCs in tumor-related immunosuppression. We will specifically look into the molecular immune-related mechanisms in the tumor microenvironment that result in reduced drug sensitivity and tumor relapse, as well as methods for combating drug resistance and focusing on immunosuppressive tumor networks. DCs play a crucial role in modulating the immune response. Especially, as cancer progresses, DCs may switch from playing an immunostimulatory to an inhibitory role. This article’s main emphasis is on tumor-infiltrating DCs. We address how they affect tumor growth and expansion, and we highlight innovative approaches for therapeutic modulation of these immunosuppressive DCs which is necessary for future personalized therapeutic approaches.

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Antigen presentation by mouse monocyte-derived cells: Re-evaluating the concept of monocyte-derived dendritic cells

Cited by 16 publications

References 56 publications

CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes

CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes

Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues

Tumor-Infiltrating Dendritic Cells: Decisive Roles in Cancer Immunosurveillance, Immunoediting, and Tumor T Cell Tolerance

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