Antigen Presentation and Cytotoxic T Lymphocyte Killing Studied in Individual, Living Cells

Hahn, Klaus M.; DeBiasio, Richard; Tishon, Antoinette; Lewicki, Hanna; Gairin, Jean Edouard; LaRocca, Greg; Taylor, D. Lansing; Oldstone, Michael B. A.

doi:10.1006/viro.1994.1298

Cited by 19 publications

(12 citation statements)

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“…We have confirmed the observations of Sanderson and subsequent researchers (14,24,25) that CTL cytotoxicity is characterized by the rapid zeiotic death of the target cells after highly variable browse times, which can be as short as 8 min or in excess of 9 hr (Table 2). Although it is known that CTL-triggered apoptotic death involves a complex multicomponent enzyme cascade (26), this variability of browse times remains puzzling.…”

Section: Discussionsupporting

confidence: 76%

Variant antigenic peptide promotes cytotoxic T lymphocyte adhesion to target cells without cytotoxicity

Shotton

Attaran

1998

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionsupporting

confidence: 76%

Variant antigenic peptide promotes cytotoxic T lymphocyte adhesion to target cells without cytotoxicity

Shotton

Attaran

1998

Proc. Natl. Acad. Sci. U.S.A.

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“…Although the BalbCl7 and MC57 cell lines are not considered professional APCs, they have been used extensively for antigen presentation in cytotoxic T-cell recognition and killing assays [43], [44], [45].…”

Section: Resultsmentioning

confidence: 99%

Interaction of Cowpea Mosaic Virus (CPMV) Nanoparticles with Antigen Presenting Cells In Vitro and In Vivo

et al. 2009

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BackgroundPlant viruses such as Cowpea mosaic virus (CPMV) are increasingly being developed for applications in nanobiotechnology including vaccine development because of their potential for producing large quantities of antigenic material in plant hosts. In order to improve efficacy of viral nanoparticles in these types of roles, an investigation of the individual cell types that interact with the particles is critical. In particular, it is important to understand the interactions of a potential vaccine with antigen presenting cells (APCs) of the immune system. CPMV was previously shown to interact with vimentin displayed on cell surfaces to mediate cell entry, but the expression of surface vimentin on APCs has not been characterized.MethodologyThe binding and internalization of CPMV by several populations of APCs was investigated both in vitro and in vivo by flow cytometry and fluorescence confocal microscopy. The association of the particles with mouse gastrointestinal epithelium and Peyer's patches was also examined by confocal microscopy. The expression of surface vimentin on APCs was also measured.ConclusionsWe found that CPMV is bound and internalized by subsets of several populations of APCs both in vitro and in vivo following intravenous, intraperitoneal, and oral administration, and also by cells isolated from the Peyer's patch following gastrointestinal delivery. Surface vimentin was also expressed on APC populations that could internalize CPMV. These experiments demonstrate that APCs capture CPMV particles in vivo, and that further tuning the interaction with surface vimentin may facilitate increased uptake by APCs and priming of antibody responses. These studies also indicate that CPMV particles likely access the systemic circulation following oral delivery via the Peyer's patch.

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“…Because of their large nuclei and limited cytoskeleton, these changes are difficult to observe in the Jurkat T cells. We therefore turned to another Fas-mediated apoptotic system, that of CHO (Chinese hamster ovary) cell kil]ing by cytotoxic T lymphocytes, where the large well-spread target CHO cell could be readily observed by DIC microscopic imaging (Hahn et al, 1994). Examination of the morphological changes accompanying apoptosis in response to killing of targeted CHO cells by cytotoxic T lymphocytes reveals a series of changes typical of the apoptotic death response.…”

Section: Cdc42 Modifies the Nornal Morphological Program In Apoptoticmentioning

confidence: 99%

“…The morphology changes characteristic of apoptotic Jurkat cells were routinely assessed by staining cells in a solution of 2 ,Lg/ml acridine orange plus 2 ,ug/ml ethidium bromide and visualization with fluorescence microscopy as described (McGahon et al, 1995;Waters et al, 1996). Cytotoxic T cell (CTL) killing, differential interface contrast (DIC) microscopy, and image processing procedures were performed as described previously (Hahn et al, 1994), except that the cells were maintained in a sealed Dvorak chamber heated to 37°C by a temperature-controlled air curtain.…”

Section: Analysis Of Apoptosismentioning

confidence: 99%

The small GTPase Cdc42 initiates an apoptotic signaling pathway in Jurkat T lymphocytes.

Chuang

Hahn

Lee

et al. 1997

MBoC

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Apoptosis plays an important role in regulating development and homeostasis of the immune system, yet the elements of the signaling pathways that control cell death have not been well defined. When expressed in Jurkat T cells, an activated form of the small GTPase Cdc42 induces cell death exhibiting the characteristics of apoptosis. The death response induced by Cdc42 is mediated by activation of a protein kinase cascade leading to stimulation of c-Jun amino terminal kinase (JNK). Apoptosis initiated by Cdc42 is inhibited by dominant negative components of the JNK cascade and by reagents that block activity of the ICE protease (caspase) family, suggesting that stimulation of the JNK kinase cascade can lead to caspase activation. The sequence of morphological events observed typically in apoptotic cells is modified in the presence of activated Cdc42, suggesting that this GTPase may account for some aspects of cytoskeletal regulation during the apoptotic program. These data suggest a means through which the biochemical and morphological events occurring during apoptosis may be coordinately regulated.

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Antigen Presentation and Cytotoxic T Lymphocyte Killing Studied in Individual, Living Cells

Cited by 19 publications

References 0 publications

Variant antigenic peptide promotes cytotoxic T lymphocyte adhesion to target cells without cytotoxicity

Variant antigenic peptide promotes cytotoxic T lymphocyte adhesion to target cells without cytotoxicity

Interaction of Cowpea Mosaic Virus (CPMV) Nanoparticles with Antigen Presenting Cells In Vitro and In Vivo

The small GTPase Cdc42 initiates an apoptotic signaling pathway in Jurkat T lymphocytes.

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