Antigen-Nachweis des „Marburg-Virus” in den Organen infizierter Meerschweinchen durch Immunfluoreszenz

Forty Years of Marburg Virus

“…Antigen detection of MARV was also reported in 1968 [3]. Reprints of the original papers were sent to all the foreign institutions involved in the efforts to find the unknown agent of this new disease.…”

Canine Distemper Virus

“…Using COONS' direct immunofluorescence technique, virus specific antigen was first demonstrated in cytoplasmic inclusions in histologic preparations from patients and experimentally infected animals and in cell cultures (SIEGERT et al, , 1968 c;SLENCZKA et al , 1968;HAAS et al, 1968 b;CARTER and BRIGHT, 1968;HOFMANN and KUNZ, 1971). The antigen inclusions (Figs.…”

“…After injection of patients' blood or of tissue suspensions taken during the febrile stage the animals became sick and developed a high fever (SMITH et al, 1967;SIEGERT et al, 1967 a, b;. With the aid of fluorescent dye-marked immune sera from patients and experimental animals, cytoplasmic inclusions were demonstrated in tissue preparations from affected guinea pigs and patients, as well as in infected cell cultures (SIEGERT et al, 1967 b;1968 b, c;SLENCZKA et al, 1968). Reinfection of convalescent guinea pigs induced no febrile reaction, indicating the presence of an effective immunity.…”

Marburg Hemorrhagic Fever: Report of a Case Studied by Immunohistochemistry and Electron Microscopy

“…This case demonstrates the value of transmission electron microscopy as a tool for assisting in the definitive diagnosis of Marburg or Ebola hemorrhagic fever, as well as providing insight into the pathogenesis of these agents. lmmunofluorescence studies in liver in 1 of these cases [16] and kidney in a second case [5] were reported, but immunohistochemistry was not performed on tissues from any of these cases, and ultrastructural studies were very limited. Detailed examination of human tissues would be useful for validating available animal model information and would provide a better understanding of the pathogenesis of filoviruses.…”