Antigen microarrays: descriptive chemistry or functional immunomics?

Prechl, József; Papp, Krisztián; Erdei, Anna

doi:10.1016/j.it.2010.01.004

Cited by 29 publications

(21 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, immune targets in both murine and human Salmonellosis are poorly characterized (30,31), inhibiting detailed examination of immunity and preclinical vaccine development. However, proteomic microarrays can now be constructed containing complete or partial pathogen proteomes and can provide a comprehensive overview of antigenic targeting using sera from infected animals or patients (32)(33)(34)(35)(36).…”

mentioning

confidence: 99%

Identification of a common immune signature in murine and human systemic Salmonellosis

Lee¹,

Liang

Juárez

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Despite the importance of Salmonella infections in human and animal health, the target antigens of Salmonella -specific immunity remain poorly defined. We have previously shown evidence for antibody-mediating protection against invasive Salmonellosis in mice and African children. To generate an overview of antibody targeting in systemic Salmonellosis, a Salmonella proteomic array containing over 2,700 proteins was constructed and probed with immune sera from Salmonella -infected mice and humans. Analysis of multiple inbred mouse strains identified 117 antigens recognized by systemic antibody responses in murine Salmonellosis. Importantly, many of these antigens were independently identified as target antigens using sera from Malawian children with Salmonella bacteremia, validating the study of the murine model. Furthermore, vaccination with SseB, the most prominent antigenic target in Malawian children, provided mice with significant protection against Salmonella infection. Together, these data uncover an overlapping immune signature of disseminated Salmonellosis in mice and humans and provide a foundation for the generation of a protective subunit vaccine.

show abstract

mentioning

confidence: 99%

Identification of a common immune signature in murine and human systemic Salmonellosis

Lee¹,

Liang

Juárez

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…As for immunoglobulins, except for IgD, all classes and subclasses have been shown to be detectable on antigen arrays [7]. Therefore the choice mainly depends on the purpose of the study; we have mostly used IgG detection for the assessment of vaccination effects and to monitor autoreactivity.…”

Section: Interpreting Complement Activation As a Function Of Antibodymentioning

confidence: 99%

Complementing antibody profiles: Assessing antibody function on antigen microarrays

2012

Self Cite

View full text Add to dashboard Cite

Antibody effector functions other than neutralization depend on interactions with soluble and cellular components of the immune system. Antigen recognition is usually oligoclonal, with the different clones of antibodies belonging to different classes, subclasses, glycoforms and having different affinities and epitope specificities. Thus, composition of immune complexes determines biological effects mainly via interactions with FcR and complement proteins. Antibodies are capable of triggering any of the three pathways of complement activation and antigen recognition of complex antigens often results in the activation of more than one pathway. These events can be tracked in a multiplex format using antigen microarrays, where complement products bind to elements of the microarray. By controlling cation concentrations and detecting various complement components (C1q, C4, C3) contribution of the different pathways can be identified. Parallel measurement of antibodies and complement proteins provides a novel way of looking at interactions between antigen and antibodies. We propose the use of immune complex signatures, composite depictions of antibody and complement content of immune complexes characterizing healthy and diseased populations. Normalized interquartile ranges of antibody binding (IgM, IgG) and complement deposition (C4, C3) are projected onto radar charts to produce patterns that can distinguish normal and altered immune responses.The comprehensive interaction studies of serum antibodies and complement with arrays of antigens can generate profiles for a better understanding of disease mechanism.1 Antibodies, complement and arrayed antigenThe complement system participates in wide ranging biological phenomena, starting from regulation of organogenesis and regeneration through mediation of inflammation to promoting opsonic phagocytosis to modulation of adaptive immunity. This variability is partly due to the fact that the system has about 30 components and also due to the presence of a variety of receptors on different cell types. While complement activation usually implies the triggering of one of the pathways which then proceeds to the formation of the membrane attack complex, it is important to note that the cascade of enzymatic cleavage events can be blocked at several points by natural inhibitory and regulatory molecules, producing a different outcome. Antibodies are usually associated with classical pathway, where antigen-bound IgM or IgG provides binding sites for C1q, leading to activation of the associated serine proteases C1r and C1s. However, it is important to note that not only classical but also the lectin and alternative pathways can Otherwise, epitope density on the microarray can be controlled by adjusting concentration of the antigen solution used for array printing. At a given epitope density antibodies with higher affinity will be more potent complement activators as the time spent in antigen-bound form will be longer. Therefore, on part of the antigen, quality and epitope densit...

show abstract

“…Systems-level analysis of gene expression using DNA microarrays has had a tremendous impact on cellular and molecular biology; the potential now exists for proteomic microarrays to have a similar influence on immunological research [28,29]. A substantial amount of work has been done to apply multiplexed highthroughput protein microarrays for the serological profiling and analysis of antibody responses; this research has ranged from infectious disease to cancer to autoimmune disease [30].…”

Section: Microarrays For Studying Protein-protein Interactions In Thementioning

confidence: 99%

Systems analysis of adaptive immunity by utilization of high-throughput technologies

Reddy

Georgiou

2011

Current Opinion in Biotechnology

View full text Add to dashboard Cite

Antigen microarrays: descriptive chemistry or functional immunomics?

Cited by 29 publications

References 40 publications

Identification of a common immune signature in murine and human systemic Salmonellosis

Identification of a common immune signature in murine and human systemic Salmonellosis

Complementing antibody profiles: Assessing antibody function on antigen microarrays

Systems analysis of adaptive immunity by utilization of high-throughput technologies

Contact Info

Product

Resources

About