Antigen expression of human eccrine sweat glands

Abstract

Human eccrine sweat glands express CK7, CK8, CK14, CK18, CK19, CEA, EMA, Ki67, p63, EGF and EGFR. In skin, CEA can be used as a specific immunological marker of sweat glands.

In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands

“…As well, we found CK7-, CK14-, CK19-and CEA-positive cells in them. These positive results are consistent to the markers of early developmental stage of sweat glands we detected before [17]. This finding is consistent with the previous hypothesis that sweat glands could be integrated within engineered skin constructs by incorporation of SGCs and sustained release of EGF contributing to the epithelial ingrowth and sweat gland formation.…”

“…Cells that can develop into sweat glands can be characterized by differential expression of combinations of cytokeratin polypeptides [17]. Immunohistochemical study of engineered skin constructs showed CK7-, CK14-, CK19-and CEA-positive cells in SEFC and SFC constructs; According to the appearance of sweat gland-like structure in SEFC and SFC culture, we emphasized on observing the positive cells in bud-like structure areas.…”

“…For incubation with antibodies to CK7, CK14, CK19 and CEA (Neomarker, Fremont, CA), sections were treated as we previously described [17]. Then sections were incubated at 4 C overnight with the monoclonal mouse anti-human antibodies at 1:100 dilution, then with horseradish peroxidase-conjugated goat anti-mouse IgG (Vector, Burlingame, CA, USA) for 1 h at room temperature (1:100 dilution).…”

Mesenchymal stem cells delivered in a microsphere-based engineered skin contribute to cutaneous wound healing and sweat gland repair

“…To monitor the survival of implanted cells in the wound, tissue sections were embedded in OCT and observed by fluorescence microscopy. In addition, skin sections were treated with primary antibodies for CEA (Neomarker, Fremont, CA), a sign of developing sweat glands, as we previously described [25]. Then secondary antibodies conjugated to rhodamine-isothiocyanate (Southern Biotechnology) were used for fluorescence staining detection and confocal laser scanning fluorescence microscopy (Olympus FV10i, Tokyo, Japan)…”

Axillary hyperhidrosis: A review of the extent of the problem and treatment modalities

“…17 Further studies are needed to determine the efficacy of this treatment, as evidence shows that sweat glands do not form post embryonic stage and so this treatment could offer a permanent fix to hyperhidrosis. 17,35 Microneedles Transdermal patches have been utilised in medicine to deliver drug molecules painlessly through the skin, hence gaining entry to the blood and immune system without being transported to the liver first. Due to the structure of the skin, however, molecules larger than 500Da have problems penetrating the stratum corneum.…”