Antigen experience relaxes the organisational structure of the T cell receptor repertoire

Abstract

The creation and evolution of the T cell receptor repertoire within an individual combines stochastic and deterministic processes. We systematically examine the structure of the repertoire in different T cell subsets in young, adult and LCMV infected mice, from the perspective of variable gene usage, nucleotide sequences and amino acid motifs. Young individuals share a high level of organization, especially in the frequency distribution of variable genes and amino acid motifs. In adult mice, this structure relaxes and is replaced by idiotypic evolution of the effector and regulatory repertoire. The repertoire of CD4+ regulatory T cells was more similar to naïve cells in young mice, but became more similar to effectors with age. Finally, we observed a dramatic restructuring of the repertoire following infection with LCMV. We hypothesize that the stochastic process of recombination and thymic selection initially impose a strong structure to the repertoire, which gradually relaxes following asynchronous responses to different antigens during life.

Large clones of pre-existing T cells drive early immunity against SARS-COV-2 and LCMV infection

“…Cells were sorted on a SORP-FACS-AriaII and analyzed using FACSDiva (BD Biosciences) and FlowJo (Tree Star) software. 55 Sorted cells were centrifuged (450g for 10 minutes) prior to RNA extraction.…”