Antigen detection based on background fluorescence quenching immunochromatographic assay

Chen, Xiangjun; Xu, Yangyang; Yu, Jinsheng; Li, Jiutong; Zhou, Xuelei; Wu, Chuanyong; Ji, Qiuliang; Ren, Yuan; Wang, Liqun; Huang, Zijun; Zhuang, Hanling; Piao, Linhua; Head, Richard D.; Wang, Yajie; Lou, Jiatao

doi:10.1016/j.aca.2014.07.025

Cited by 26 publications

(16 citation statements)

References 19 publications

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“…The unconjugated quenching complexes migrated to absorbent pad. No fluorescence quenching occurred on fluorescein coated NC membrane between the TL and CL [185].…”

Section: Fluorescent Quenching Materialsmentioning

confidence: 86%

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Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

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“…The unconjugated quenching complexes migrated to absorbent pad. No fluorescence quenching occurred on fluorescein coated NC membrane between the TL and CL [185].…”

Section: Fluorescent Quenching Materialsmentioning

confidence: 86%

“…The detection signal intensity of the TL increased with the increasing concentration of analyte [183]. The fluorescence quenching based LFAs has been used for ractopimine [184], Cr 3+ [183] and AFP [185] detection.…”

Section: Fluorescent Quenching Materialsmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

481

315

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“…Upon reaching the test line, the remanent GNPs-labeled antibody not binding with AFM1 or CAP bound with the coated antigen of AFM1 or CAP to form the antigen-antibody-GNP complex that quenches the fluorescence on the test line. The content of test samples was correlated to the quenching of the fluorescence presented as the ratio of fluorescence on the test line to the background fluorescence ( F 1 / F 0 ) [10]. …”

Section: Methodsmentioning

confidence: 99%

“…The method has been used for the alpha fetoprotein in clinical practice. Unlike the conventional GICA assay, in the background fluorescence quenching immunochromatographic assay (bFQICA), the fluorescence donors are fluorescein that are precoated on the entire nitrocellulose membrane and quenching occurs between the gold particles and nitrocellulose membrane [10]. In this study, we firstly applied such technique in the food safety.…”

Section: Introductionmentioning

confidence: 99%

Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay

Tian²,

et al. 2017

BioMed Research International

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Harsh demanding has been exposed on the concentration of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. In this study, we developed a new method based on background fluorescence quenching immunochromatographic assay (bFQICA) to detect AFM1 and CAP in milk. The detection limit for AFM1 was 0.0009 ng/mL, while that for the CAP was 0.0008 ng/mL. The assay variability was determined with 3 AFM1 standards (i.e., 0.25 ng/mL, 0.5 ng/mL, and 1.0 ng/mL), and the actual detection value was 0.2497, 0.5329, and 1.0941, respectively. For the assay variability of 3 CAP standards (i.e., 0.10 ng/mL, 0.30 ng/mL, and 0.50 ng/mL), the actual detection value was 0.0996, 0.3096, and 0.4905, respectively. The recovery rate of AFM1 was 99.7%–101.7%, while that for CAP was 95.3%–97.6%. For the test stability, AFM1 and CAP showed satisfactory test stability even at month 5. Compared with the sensitivity of liquid chromatography-mass spectrometry (LC-MS) method, no statistical difference was noticed in results of the bFQICA. Our method is convenient for the detection of AFM1 and CAP in milk with a test duration of about 8 minutes. Additionally, an internal WiFi facility is provided in the system allowing for quick connection and storage in the intelligent cell phone.

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“…Recently, some research groups [25][26][27][28][29][30] reported a novel uorescence-quenching ICS to improve the assay sensitivity. The uorescence donors used in the above study were mainly uo-rescein, uorescein-doped microspheres or Ru(phen) 3…”

mentioning

confidence: 99%

Quantum dot based multiplex fluorescence quenching immune chromatographic strips for the simultaneous determination of sulfonamide and fluoroquinolone residues in chicken samples

Sheng

et al. 2017

RSC Adv.

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A novel multiplex fluorescence quenching immune chromatographic strip (FQICS), using quantum dots (QDs) as a fluorescence donor, was proposed to screen sulfonamide (SA) and fluoroquinolone (FQ) residues in chicken samples, simultaneously. The principle of the proposed FQICS is on the basis of the fluorescence quenching effect, and colloidal gold was used as the fluorescence quencher. The limit of detection (LOD) in standard solution was 3 ng mL À1 and 0.6 ng mL À1 for sulfaquinoxaline (SQX) and norfloxacin (NOR), respectively, through visual detection using UV. For specificity analysis, results obtained indicated that this established method could be applied in broad-spectrum detection of SAs and FQs. Then the proposed FQICS was successfully applied in semiquantitative analysis of SQX (30 mg kg À1) and NOR (6 mg kg À1) in spiked chicken samples. The results obtained using the strips showed great agreement with those obtained using an ELISA kit, which indicates the good accuracy of the strips. As a conclusion, the proposed FQICS with a signal "turn-on" mode provides a suitable immunoassay for rapid, sensitive and simultaneous screening of SAs and FQs in chicken samples on-site.

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Antigen detection based on background fluorescence quenching immunochromatographic assay

Cited by 26 publications

References 19 publications

Lateral flow assays: Principles, designs and labels

Lateral flow assays: Principles, designs and labels

Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay

Quantum dot based multiplex fluorescence quenching immune chromatographic strips for the simultaneous determination of sulfonamide and fluoroquinolone residues in chicken samples

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