Antigen-capture enzyme-linked immunosorbent assays for detection of different H5 avian Influenza A virus

Abstract

Abstract. Avian Influenza A virus (AIV) subtype H5 is divided into American and Eurasian lineages, according to hemagglutinin gene sequences. Although methods for detecting H5 AIVs have been described, no H5 strain-specific detection method has been reported. The purpose of the present study was to develop an antigen-capture enzyme-linked immunosorbent assay (ACE) to detect and differentiate between the American and the Eurasian H5 AIVs. Monoclonal antibodies (mAbs) against the HA fragment of a Eurasian H5N2 AIV were used as the capture antibodies as well as the detector antibodies after labeling with horseradish peroxidase to develop an ACE. One mAb was selected for detecting the American as well as the Eurasian H5 AIVs. The other mAb was used for detecting only the Eurasian H5N2 but not the American H5N2 AIVs, H6N1 AIVs, or Newcastle disease virus. The ACEs developed would be useful for detection and differentiation of H5 AIVs from the Eurasian and the American H5 AIVs.

Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies

“…However, isolation and identification of viruses requires complicated procedures that are time-consuming (ranging from days to weeks) and labor-intensive . Although the ELISA has been proven to be sensitive, versatile and can be automated (Chiu et al, 2012;Watson et al, 2009), it is non-reusable. The PCR has played a crucial role in the rapid detection of avian influenza viruses (AIVs) (Bao et al, 2012;Ng et al, 2006;Spackman et al, 2002).…”