Antigen-Capture Enzyme Immunoassay: a Comparison with other Methods for the Detection of Spotted Fever Group Rickettsiae in Ticks

Radulović, Suzana; Feng, Hui; Crocquet‐Valdes, Patricia A.; Morović, Miro; Dželalija, Boris; Walker, David H.

doi:10.4269/ajtmh.1994.50.359

Cited by 16 publications

(8 citation statements)

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“…Slides are air-dried and fixed in acetone before being treated with polyclonal or monoclonal antibodies conjugated with immunofluorescent labels, as has been described for the rickettsiae [64,65]. An antigen-capture EIA has also been used for the detection of these bacteria in ticks [66]. Immunodetection methods are also available for the detection of C. burnetii [67], ehrlichiae [68,69], F. tularensis [70], and borreliae [71].…”

Section: Detection and Isolation Of Bacteria From Ticksmentioning

confidence: 99%

Ticks and Tickborne Bacterial Diseases in Humans: An Emerging Infectious Threat

Parola

Raoult

2001

Clinical Infectious Diseases

993

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Ticks are currently considered to be second only to mosquitoes as vectors of human infectious diseases in the world. Each tick species has preferred environmental conditions and biotopes that determine the geographic distribution of the ticks and, consequently, the risk areas for tickborne diseases. This is particularly the case when ticks are vectors and reservoirs of the pathogens. Since the identification of Borrelia burgdorferi as the agent of Lyme disease in 1982, 15 ixodid-borne bacterial pathogens have been described throughout the world, including 8 rickettsiae, 3 ehrlichiae, and 4 species of the Borrelia burgdorferi complex. This article reviews and illustrate various aspects of the biology of ticks and the tickborne bacterial diseases (rickettsioses, ehrlichioses, Lyme disease, relapsing fever borrelioses, tularemia, Q fever), particularly those regarded as emerging diseases. Methods are described for the detection and isolation of bacteria from ticks and advice is given on how tick bites may be prevented and how clinicians should deal with patients who have been bitten by ticks.

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Section: Detection and Isolation Of Bacteria From Ticksmentioning

confidence: 99%

Ticks and Tickborne Bacterial Diseases in Humans: An Emerging Infectious Threat

Parola

Raoult

2001

Clinical Infectious Diseases

993

728

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“…A drop of hemolymph can be placed onto a slide, air dried, and fixed in acetone before being treated with fluorescein isothiocyanate-labelled immunoglobulins against the various rickettsial groups (31). An antigen capture immunoenzyme assay with a crossreactive monoclonal antibody directed against the major surface antigen of 135-kDa has been demonstrated to be a good alternative for the primary screening of tick samples (116).…”

Section: Isolation and Detection Of Rickettsiae From Arthropodsmentioning

confidence: 99%

Laboratory diagnosis of rickettsioses: current approaches to diagnosis of old and new rickettsial diseases

1997

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“…Hoje apenas um terço dos carrapatos deste gênero parasitam répteis refletindo a história evolutiva inicial (CUPP, 1991 (MARSAUDON et al,1994). Além disto, o R. sanguineus também pode transmitir para o homem a Rickettsia conorii (RADULOVIC et al,1994). A distribuição geográfica dos agentes infecciosos transmitidos por carrapatos acompanha a distribuição dos vetores.…”

Section: Introductionunclassified