Antigen-binding T cells as helper cells. Separation of helper cells by immune rosette formation.

Abstract

The spleen T cells from mice immunized 6 days earlier with either chicken gamma globulin (CGG) or with donkey erythrocytes (DRC) were rosetted with CGG-coated sheep erythrocytes or with DRC. The immune rosettes (RFC) (antigen-binding cells) were separated from the bulk of nonrosette-forming cells (non-RFC) by 1-g velocity sedimentation and the RFC and non-RFC tested for helper activity in cooperative antihapten responses in vitro. RFC or non-RFC were mixed with normal or hapten-primed spleen cells, challenged with the appropriate hapten-carrier conjugate and cultured for 4 days in Marbrook tissue cultures. The helping activity was quantitated from the numbers of antihapten antibody-producing cells generated per culture. The results show that specific helper cell activity could be selectively recovered in the immune rosette-forming cell population whereas the non-RFC population was depleted of help. These findings indicate that the helper T cells express specific antigen binding receptors.

Antibody‐Secreting Cell Precursor Frequencies among the Sheep Erythrocyte‐Binding Cells of Non‐Immune Mice

“…The 50% loss of SRBC-ABC during purification included most of the T SRBC-ABC, which are more readily dissociated mechanically than B SRBC-ABC [9]. Although helper [22] and suppressor cells [\\] may bind antigen, their influence on B-cell development was eliminated by the limiting dilution procedure. The absence of extraneous T-cell-induced effects can also be inferred from the straight lines seen in the limiting-dilution analysis plots (Fig.…”

Adult murine T cells activated in vitro by alpha-fetoprotein and naturally occurring T cells in newborn mice: identity in function and cell surface differentiation antigens.

“…The preparation and activity of the anti-T cell serum (sheep anti-DBA/2 mouse brain) used in this study at a dilution of 1/15 have been described (17). The anti-Thy-1.2 (AKR anti-C3H) serum was used at a dilution of 1/20.…”

The nature of allorosette-forming cells in rats

“…Using sheep red blood cells as antigen, some authors could not detect helper cell activity among rosette-forming T cells [10,11]. In other experiments, T cells forming rosettes with sheep red blood cells exhibit helper cell function [3,23,37]. Also, delayed type hypersensitivity and in vitro cytotoxicity are contributed to rosette forming T cells [12].…”