Antifungal Volatilomes Mediated Defense Mechanism against Fusarium oxysporum f. sp. lycopersici, the Incitant of Tomato Wilt

Thangaraj, Praveen; Balamurali, Akshaya Subbaih; Subbiah, Krishnamoorthy Akkanna; Sevugapperumal, Nakkeeran; Thiribhuvanamala, G.; Uthandi, Sivakumar; Shanmugam, Haripriya

doi:10.3390/molecules27113631

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…In the case of Colletotrichum sp., the results obtained were higher than those reported by Priya et al [28] for other basidiomycete genera such as Auricularia polytricha (53.70%), Coprinus comatus (40%), Ganoderma lucidum (54.81%), Volvariella volvacea (42.9%), Lentinus edodes (45.5%), Pycnoporus sanguineus (57.4%), Schizophyllum commune (47.8%), and Trametes versicolor (43.63%). Regarding the results observed with Fusarium isolates, they were lower than the inhibitory activity for basidiomycetes such as G. lucidum (60.3%) but like those reported for L. edodes (35.3%) [50]. Variations among fungal species may explain these results due to differences in their evolutionary origin.…”

Section: Production Kinetics Of Antifungal Activity In Cell-free Supe...mentioning

confidence: 52%

Antagonistic Activity of Macrolepiota sp. CS185 against Post-Harvest Fungi of Fig Fruits (Ficus carica L.)

Gutiérrez-Soto,

López-Sandin,

Hernández Ochoa

et al. 2024

Microbiology Research

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Macrolepiota sp. CS185 is a basidiomycete with high potential as a biocontrol agent against various phytopathogenic fungi. Regardless of its pronounced potential as a post-harvest fungi biocontrol agent, its activity in tomato seedlings infected with Alternaria alternata has not been well studied. Thus, the present work aimed to evaluate the cultures and supernatants’ antagonistic activity against fig fruits’ post-harvest fungi and antifungal activity production kinetics. The culture antagonistic characteristics were assessed through multiple confrontations, the supernatant concentration effect, and the kinetics of antagonistic action. The multiple confrontations showed differences (p ≤ 0.05) among phytopathogens and over time, with Colletotrichum sp. 2 being the most susceptible. Based on the 9-day incubation profile, the treatment fractions supplemented with a 50% concentration of Macrolepiota sp. CS185 supernatants showed a higher inhibition percentage (%In). Except for Alternaria alternata 1 and 2, the rest of the isolates showed a similar decrease in antagonistic activity up to a certain extent over time. Among all tested strains, Colletotrichum sp. 2 was found with a higher susceptibility. Regarding the production kinetics of antagonistic activity, a triple interaction was observed between the phytopathogen, the age of the Macrolepiota sp. CS 185 culture, and incubation time. In addition, changes in the mycelium growth rate (p ≤ 0.05) along with the higher activity in the supernatants of 20 and 30 days were observed and suggested the production of multiple bioactive metabolites. These results indicate that Macrolepiota sp. CS185 produces antifungal metabolites at different times and could be a suitable candidate to control fig fruits’ post-harvest fungi issues.

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Section: Production Kinetics Of Antifungal Activity In Cell-free Supe...mentioning

confidence: 52%

Antagonistic Activity of Macrolepiota sp. CS185 against Post-Harvest Fungi of Fig Fruits (Ficus carica L.)

Gutiérrez-Soto,

López-Sandin,

Hernández Ochoa

et al. 2024

Microbiology Research

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“…Therefore, the antimicrobial compound from the cap portion of the fruiting body was characterized through GC-MS analysis. In this study, the Trace GC Ultra and DSQII model MS from Thermo Fisher Scientific Limited, Markha, Canada were engaged for analysis [ 9 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

Benzothiazole—An Antifungal Compound Derived from Medicinal Mushroom Ganoderma lucidum against Mango Anthracnose Pathogen Colletotrichum gloeosporioides (Penz and (Sacc.))

et al. 2023

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The present investigation is focused on exploring the possibilities of identifying biomolecules from the fruiting body of the medicinal mushroom Ganoderma lucidum against the mango anthracnose pathogen Colletotrichum gloeosporioides. The fruiting body (cap and stipe portion) of G. lucidum extracted with ethyl acetate solvent at a maximum inhibitory concentration of 1 percent exhibited the maximum mycelial growth inhibition of C. gloeosporioides with 70.10 percent and 40.77 percent, respectively. Furthermore, subjecting the ethyl acetate extracts from the cap portion of G. lucidum through thin layer chromatography (TLC) revealed the presence of two bands with Rf values of 0.38 and 0.35. The compounds eluted from band 1 recorded with the maximum mycelial growth inhibition of C. gloeosporioides by 53.77 percent followed by band 2 (46.33 percent) using an agar well diffusion test. Similarly, the analysis of ethyl acetate extracts from the cap portion of G. lucidum through Gas Chromatography-Mass spectroscopy (GC-MS) revealed the presence of the organoheterocyclic compound benzothiazole, as expressed in the highest peak area at 22.03 RT with the highest probability percentage (97%). Confirmation of the antifungal nature of benzothiazole was obtained by testing the standard sample of benzothiazole which showed a cent percent of inhibition on mycelial growth of C. gloeosporioides at 50 ppm minimum fungicidal concentration. Furthermore, benzothiazole caused abnormality in the mycelial structures, viz., distortion, shrinkage, clumping of mycelium, conidial malformation, and complete arrestment of conidial germination of C. gloeosporioides as observed through Scanning Electron Microscopy. The research on biomolecular extract of G. lucidum could be a novel and interesting concept for the possibility in suppression of plant pathogenic microbes in the natural field.

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Volatile Formulation of Mentha spicata Induced Defense Mechanism in Tomato and Suppressed Damping Off Disease Caused by Pythium aphanidermatum

Praveen,

Gurudevan,

Nallusamy

et al. 2023

Indian J Microbiol

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Antifungal Volatilomes Mediated Defense Mechanism against Fusarium oxysporum f. sp. lycopersici, the Incitant of Tomato Wilt

Cited by 3 publications

References 35 publications

Antagonistic Activity of Macrolepiota sp. CS185 against Post-Harvest Fungi of Fig Fruits (Ficus carica L.)

Antagonistic Activity of Macrolepiota sp. CS185 against Post-Harvest Fungi of Fig Fruits (Ficus carica L.)

Benzothiazole—An Antifungal Compound Derived from Medicinal Mushroom Ganoderma lucidum against Mango Anthracnose Pathogen Colletotrichum gloeosporioides (Penz and (Sacc.))

Volatile Formulation of Mentha spicata Induced Defense Mechanism in Tomato and Suppressed Damping Off Disease Caused by Pythium aphanidermatum

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