Antifungal Susceptibility of 182 Fusarium Species Isolates from 20 European Centers: Comparison between EUCAST and Gradient Concentration Strip Methods

Normand, Anne‐Cécile; Imbert, Sébastien; Al‐Hatmi, Abdullah M. S.; Chryssanthou, Erja; Cassaing, Sophie; Schuttler, Christine; Hasseine, Lilia; Mahinc, Caroline; Costa, Damien; Bonnal, Christine; Ranque, Stéphane; Sautour, Marc; Rubio, Elisa; Delhaès, Laurence; Riat, Arnaud; Sendid, Boualem; Kristensen, Lise; Brandenberger, Marcel; Stubbe, Dirk; Brun, Sophie; Piarroux, Renaud; Fekkar, Arnaud

doi:10.1128/aac.01495-21

Cited by 10 publications

(13 citation statements)

References 15 publications

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“…This is consistent with the findings of most studies in which amphotericin B was found to have lower MICs in vitro compared to other antifungals [4,13,[35][36][37]. As previously reported [10,31,38], the susceptibility of Fusarium to amphotericin B varied, depending on the species. Surprisingly, we observed miltefosine was found to be the most active agent against resistant and susceptible Fusarium isolates after amphotericin B. Miltefosine was initially developed as an anticancer agent, but it is now a clinically approved anti-parasitic drug against Leishmania species.…”

Section: Discussionsupporting

confidence: 92%

In Vitro Antifungal Susceptibility Profile of Miltefosine against a Collection of Azole and Echinocandins Resistant Fusarium Strains

Nosratabadi

Akhtari

Faeli

et al. 2022

JoF

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Fusarium species are filamentous fungi that cause a variety of infections in humans. Because they are commonly resistant to many antifungal drugs currently available in clinical settings, research into alternative targets in fungal cells and therapeutic approaches is required. The antifungal activity of miltefosine and four comparators, amphotericin B, voriconazole, itraconazole, and caspofungin, were tested in vitro against a collection of susceptible and resistant clinical (n = 68) and environmental (n = 42) Fusarium isolates. Amphotericin B (0.8 μg/mL) had the lowest geometric mean (GM) MICs/MECs values followed by miltefosine (1.44 μg/mL), voriconazole (2.15 μg/mL), caspofungin (7.23 μg/mL), and itraconazole (14.19 μg/mL). Miltefosine was the most effective agent against Fusarium isolates after amphotericin B indicating that miltefosine has the potential to be studied as a novel treatment for Fusarium infections.

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Section: Discussionsupporting

confidence: 92%

In Vitro Antifungal Susceptibility Profile of Miltefosine against a Collection of Azole and Echinocandins Resistant Fusarium Strains

Nosratabadi

Akhtari

Faeli

et al. 2022

JoF

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“…When evaluating potential cut-off values for the definition of reduced susceptibility, significant variation is noted across three primary species complexes based around Fusarium solani, Fusarium oxysporum, and Fusarium verticillioides , although this does not encompass all pathogenic species. 102 , 103 In spite of this variability and high rates of resistance to the most commonly used agents (amphotericin B, mould-active triazoles), cure is feasible and in vitro resistance is not consistently predictive of clinical outcomes; resolution of neutropenia was associated with a greater likelihood of clinical success. 104 , 105 This discrepancy emphasizes the need to devise a means for prospective trials aimed at these rare moulds to achieve better data-driven treatment approaches.…”

Section: Methods Of Antifungal Resistance Testingmentioning

confidence: 99%

Clinical utility of antifungal susceptibility testing

McCarty

Luethy

Baddley

et al. 2022

JAC-Antimicrobial Resistance

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Invasive fungal diseases cause significant morbidity and mortality, in particular affecting immunocompromised patients. Resistant organisms are of increasing importance, yet there are many notable differences in the ability to both perform and interpret antifungal susceptibility testing compared with bacteria. In this review, we will highlight the strengths and limitations of resistance data of pathogenic yeasts and moulds that may be used to guide treatment and predict clinical outcomes.

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“…Infections by species of the FSSC are problematic because most are innately resistant to mainstream azole antifungals, including voriconazole [ 29 , 30 ], which is the recommended treatment option for invasive fusariosis (IF) [ 31 , 32 ]. Amphotericin B seems to be the only antifungal to which most FSSC species are susceptible [ 29 , 30 ]. This hinders the efficacy of treating immunocompromised patients with IF [ 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Morphology, Phenotype, and Molecular Identification of Clinical and Environmental Fusarium solani Species Complex Isolates from Malaysia

James

Santhanam

Zakaria

et al. 2022

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Fusarium infections in humans (fusariosis) and in economically important plants involve species of several Fusarium species complexes. Species of the Fusarium solani species complex (FSSC) are the most frequent cause of human fusariosis. The FSSC comprises more than 60 closely related species that can be separated into three major clades by multi-locus sequence typing (MLST) using translation elongation factor 1-alpha (TEF1-α) and RNA polymerase II (RPB2) DNA sequences. The MLST nomenclature for clade 3 of the FSSC assigns numbers to species types (e.g., FSSC 2) and lowercase letters to identify unique haplotypes. The aim of this study was to analyse the genotypic and phenotypic characteristics of 15 environmental and 15 clinical FSSC isolates from Malaysia. MLST was used for the genotypic characterisation of FSSC isolates from various locations within Malaysia, which was complemented by their morphological characterisation on potato dextrose and carnation leaf agar. MLST identified eight different FSSC species: thirteen Fusarium keratoplasticum (i.e., FSSC 2), six Fusarium suttonianum (FSSC 20), five Fusarium falciforme (FSSC 3+4), two Fusarium cyanescens (FSSC 27), and one each of Fusarium petroliphilum (FSSC 1), Fusarium waltergamsii (FSSC 7), Fusarium sp. (FSSC 12), and Fusarium striatum (FSSC 21). Consistent with previous reports from Malaysia, most (11 of 15) clinical FSSC isolates were F. keratoplasticum and the majority (9 of 15) of environmental isolates were F. suttonianum (5) or F. falciforme (4) strains. The taxonomic relationships of the isolates were resolved phylogenetically. The eight Fusarium species also showed distinct morphological characteristics, but these were less clearly defined and reached across species boundaries. Although TEF1-α and RPB2 sequences were sufficient for the species identification of most FSSC isolates, a more precise MLST scheme needs to be established to reliably assign individual isolates of the species-rich FSSC to their geographically-, epidemiologically-, and host-associated sub-lineages.

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Antifungal Susceptibility of 182 Fusarium Species Isolates from 20 European Centers: Comparison between EUCAST and Gradient Concentration Strip Methods

Cited by 10 publications

References 15 publications

In Vitro Antifungal Susceptibility Profile of Miltefosine against a Collection of Azole and Echinocandins Resistant Fusarium Strains

In Vitro Antifungal Susceptibility Profile of Miltefosine against a Collection of Azole and Echinocandins Resistant Fusarium Strains

Clinical utility of antifungal susceptibility testing

Morphology, Phenotype, and Molecular Identification of Clinical and Environmental Fusarium solani Species Complex Isolates from Malaysia

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