Antifungal Properties of Two Volatile Organic Compounds on Barley Pathogens and Introduction to Their Mechanism of Action

Kaddes, Amine; Fauconnier, Marie‐Laure; Sassı, Khaled; Nasraoui, B.; Jijakli, M. Haïssam

doi:10.3390/ijerph16162866

Cited by 9 publications

(6 citation statements)

References 22 publications

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“…The VOCs of Saccharomyces cerevisiae increased the lipid peroxidation of the pathogen Phyllosticta citricarpa and, consequently, decreased membrane fluidity, increased permeability to H + and other ions and eventual cellular rupture, which directly affected the progress of the disease symptoms on plants. Such results corroborate the increase in membrane permeability of pathogenic spores of F. culmorum and Cochliobolus sativus as a result of the decrease in the efflux of K + ions into the intracellular space caused by the compounds methyl propanoate and methyl prop-2-enoate ( Kaddes et al, 2019 ). Moreover, volatiles emitted by B. subtilis and the pure compound benzothiazole affected the membrane ergosterol content of Monilinia fructicola and inhibited the activity of the enzymes pectinase and cellulase, which have an important role in cell wall and cell membrane integrity ( Zhou et al, 2019 ).…”

Section: Vocs Cause Several Molecular Changes On Phytopathogenssupporting

confidence: 78%

The power of the smallest: The inhibitory activity of microbial volatile organic compounds against phytopathogens

et al. 2023

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Plant diseases caused by phytopathogens result in huge economic losses in agriculture. In addition, the use of chemical products to control such diseases causes many problems to the environment and to human health. However, some bacteria and fungi have a mutualistic relationship with plants in nature, mainly exchanging nutrients and protection. Thus, exploring those beneficial microorganisms has been an interesting and promising alternative for mitigating the use of agrochemicals and, consequently, achieving a more sustainable agriculture. Microorganisms are able to produce and excrete several metabolites, but volatile organic compounds (VOCs) have huge biotechnology potential. Microbial VOCs are small molecules from different chemical classes, such as alkenes, alcohols, ketones, organic acids, terpenes, benzenoids and pyrazines. Interestingly, volatilomes are species-specific and also change according to microbial growth conditions. The interaction of VOCs with other organisms, such as plants, insects, and other bacteria and fungi, can cause a wide range of effects. In this review, we show that a large variety of plant pathogens are inhibited by microbial VOCs with a focus on the in vitro and in vivo inhibition of phytopathogens of greater scientific and economic importance in agriculture, such as Ralstonia solanacearum, Botrytis cinerea, Xanthomonas and Fusarium species. In this scenario, some genera of VOC-producing microorganisms stand out as antagonists, including Bacillus, Pseudomonas, Serratia and Streptomyces. We also highlight the known molecular and physiological mechanisms by which VOCs inhibit the growth of phytopathogens. Microbial VOCs can provoke many changes in these microorganisms, such as vacuolization, fungal hyphal rupture, loss of intracellular components, regulation of metabolism and pathogenicity genes, plus the expression of proteins important in the host response. Furthermore, we demonstrate that there are aspects to investigate by discussing questions that are still not very clear in this research area, especially those that are essential for the future use of such beneficial microorganisms as biocontrol products in field crops. Therefore, we bring to light the great biotechnological potential of VOCs to help make agriculture more sustainable.

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Section: Vocs Cause Several Molecular Changes On Phytopathogenssupporting

confidence: 78%

The power of the smallest: The inhibitory activity of microbial volatile organic compounds against phytopathogens

et al. 2023

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“…Fungal VOCs increase plant protection by inducing host defense systems and resistance against pathogens via different mechanisms ( Werner et al., 2016 ). One important mechanism is to change the balance of K + ions flow and disturb the pH gradient, which inhibits fungal mycelial growth and spore germination ( Kaddes et al., 2019b ). Naphthalene and monoterpenes (p-cymene, 3-carene) produced in tomato roots in response to F. oxysporum have antibacterial effects ( Gulati et al., 2020 ).…”

Section: Volatile Organic Compounds In Plant–pathogenic Fungi Interac...mentioning

confidence: 99%

“…Two organic esters (methyl pro-2-enoate and methyl propanoate) suppress mycelial growth of the fungi Fusarium culmorum and Cochliobolus sativus when in direct contact, whereas with indirect contact, the VOCs cause a decrease in the outflow of K + ions into the intracellular medium and an increase in the permeability of pathogenic spore membranes ( Kaddes et al., 2019b ). Because activity of proton pumps must guarantee the efflux of H + ions into the intracellular medium to retain electrical charges on either side of the membrane at equilibration in order to adjust for K + imbalance, dramatic changes can occur in the pH of the intracellular medium and prohibit spore germination ( Kaddes et al., 2019b ). Therefore, roles of root volatiles in regulating belowground microbiomes via effects on microbial communities and attraction of beneficial microbial species have been the focus of research.…”

Section: Volatile Organic Compounds In Plant–pathogenic Fungi Interac...mentioning

confidence: 99%

Volatile organic compounds shape belowground plant–fungi interactions

Duc

Doan

et al. 2022

Front. Plant Sci.

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Volatile organic compounds (VOCs), a bouquet of chemical compounds released by all life forms, play essential roles in trophic interactions. VOCs can facilitate a large number of interactions with different organisms belowground. VOCs-regulated plant-plant or plant-insect interaction both below and aboveground has been reported extensively. Nevertheless, there is little information about the role of VOCs derived from soilborne pathogenic fungi and beneficial fungi, particularly mycorrhizae, in influencing plant performance. In this review, we show how plant VOCs regulate plant-soilborne pathogenic fungi and beneficial fungi (mycorrhizae) interactions. How fungal VOCs mediate plant–soilborne pathogenic and beneficial fungi interactions are presented and the most common methods to collect and analyze belowground volatiles are evaluated. Furthermore, we suggest a promising method for future research on belowground VOCs.

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“…Over the years, various biological agents have been applied extensively to control diseases from pests and phytopathogens, which is known as biocontrol [ 18 ]. Essential oils are considered as biocontrol agents inhibiting phytopathogen growth on fruit surfaces by in vitro and in vivo assay during the storage period [ 19 ]. The use of essential oils controlling postharvest phytopathogens is relatively inexpensive and may not require a sophisticated system.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo antifungal activity of Cuminum cyminum essential oil against Aspergillus aculeatus causing bunch rot of postharvest grapes

2020

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Bunch rot in grapes is an aggressive disease and needs to be controlled during the postharvest period. We investigate the antifungal potential of Zanthoxylum bungeanum Maxim., Zanthoxylum rhetsa, Cuminum cyminum, Coriandrum sativum, and Zingiber montanum (J. Koenig) Link ex A. Dietr. essential oils against Aspergillus aculeatus that cause bunch rot disease on postharvest grapes. C. cyminum essential oil exhibited stronger significantly inhibition percentage of 95.08% than other treatments in in vitro assay. Cumin aldehyde (33.94%) and α-terpinen-7-al (32.20%) were identified as major volatile compounds in C. cyminum oil. Antifungal potential of C. cyminum oil was then tested in conidia germination and in vitro tests compared to cumin aldehyde and α-terpinen-7-al. Their EC50 values against the conidial germination were also estimated. Significant reduction of conidia germination was also detected in C. cyminum essential oil and cumin aldehyde at a concentration of 1,000 and 100 μg/mL, respectively. EC50 values of the C. cyminum essential oil, cumin aldehyde, and α-terpinen-7-al were 67.28 μg/mL, 9.31 μg/mL, and 13.23 μg/mL, respectively. In vivo assay, the decrease of the disease severity (0.69%) and incidence (1.48%) percentage of A. aculeatus on grape berries treated at 1,000 μg/mL of C. cyminum essential oil was significantly greater than that obtained from other treatments after 10 days incubation. In addition, grape berries treated with C. cyminum essential oil decreased weight loss and retained fruit firmness. The changing of total soluble solids, total phenolic content, and antioxidant activity are also delayed in treated fruits. Therefore, essential oil of C. cyminum may be applied as a biological antifungal agent to control A. aculeatus in postharvest grapes without any negative effects on its quality.

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Antifungal Properties of Two Volatile Organic Compounds on Barley Pathogens and Introduction to Their Mechanism of Action

Cited by 9 publications

References 22 publications

The power of the smallest: The inhibitory activity of microbial volatile organic compounds against phytopathogens

The power of the smallest: The inhibitory activity of microbial volatile organic compounds against phytopathogens

Volatile organic compounds shape belowground plant–fungi interactions

In vitro and in vivo antifungal activity of Cuminum cyminum essential oil against Aspergillus aculeatus causing bunch rot of postharvest grapes

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