Antifungal Properties, Abiotic Stress Resistance, and Biocontrol Ability of Bacillus mojavensis PS17

Diabankana, Roderic Gilles Claret; Afordoanyi, Daniel Mawuena; Сафин, Радик; Nizamov, Rustam; Karimova, L.Z.; Validov, Shamil

doi:10.1007/s00284-021-02578-7

Cited by 23 publications

(13 citation statements)

References 42 publications

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“…Overall, Ascochyta was much more susceptible to our bacterial agents than Fol. Similar results were also reported by Diabankana et al (2021) where the B. mojavensis PS17 strain demonstrated strong inhibition against several phytopathogens including Fusarium sp. and A. pisi.…”

Section: Discussionsupporting

confidence: 89%

Journal of Plant Protection Research

Rafanomezantsoa,

Gharbi,

Karkachi

et al. 2022

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The aim of this study is to find bacterial strains with antagonistic effects against Fusarium oxysporum f. sp. lycopersici (Fol) and Ascochyta sp, which are phytopathogens responsible for fusarium wilt of tomato and ascochyta blight of peas, respectively. One hundred thirty-six bacteria isolated from the rhizosphere of tomatoes were screened. Five strains with the largest inhibition zones were selected. These strains were identified by the phenotypic method, later confirmed by sequencing of 16S rDNA. All strains belonged to the genus Bacillus spp. Their inhibition capacity was evaluated by the direct method by doing a dual culture, the inhibition rates ranged from 44.32 ± 0.8 to 61.36 ± 0.2 against Fol and 62.04 ± 0.8 to 74.1 ± 0.2% against Ascochyta sp. They were then evaluated by the indirect method by evaluating, on one hand, the antifungal effect of the volatile compounds produced by the strains and on the other hand, the antifungal effect of the filtrates. The results showed that volatile compounds inhibited plant pathogens' growth with average inhibition rates of 55% against Fol and 17% against Ascochyta. For filtrates, the average inhibition rates were 33.01% against Fol and 33.74% against Ascochyta sp. Finally, the plant growth promoting rhizobacteria (PGPR) effect of B. halotolerans RFP57 was evaluated. This involved assessing their ability to stimulate the germination of tomato seeds and the growth of their vegetative organs. The results showed significant improvement in treated seedlings compared to controls. All these results show that the strains selected for this study have the potential for use as a biocontrol agent. However, it is clear that further in-depth studies are needed to confirm their true potentiality.

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Section: Discussionsupporting

confidence: 89%

Journal of Plant Protection Research

Rafanomezantsoa,

Gharbi,

Karkachi

et al. 2022

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“…Microbial pesticide control effect, production of raw materials and active ingredients are natural products, safe, non-toxic residue-free, to ensure sustainable development ( Starnes et al, 1993 ; Fravel, 2005 ). Bacillus , as one of the most important microbial pesticides, exerts inhibitory effects through mechanisms such as competitive action, antagonism, and the induction of plant resistance ( Bacon et al, 2001 ; Durairaj et al, 2017 ; Hasan et al, 2020 ; Diabankana et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Antifungal mechanisms of the antagonistic bacterium Bacillus mojavensis UTF-33 and its potential as a new biopesticide

Zhang¹,

Yang²,

Zhang³

et al. 2023

Front. Microbiol.

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Biological control has gradually become the dominant means of controlling fungal disease over recent years. In this study, an endophytic strain of UTF-33 was isolated from acid mold (Rumex acetosa L.) leaves. Based on 16S rDNA gene sequence comparison, and biochemical and physiological characteristics, this strain was formally identified as Bacillus mojavensis. Bacillus mojavensis UTF-33 was sensitive to most of the antibiotics tested except neomycin. Moreover, the filtrate fermentation solution of Bacillus mojavensis UTF-33 had a significant inhibitory effect on the growth of rice blast and was used in field evaluation tests, which reduced the infestation of rice blast effectively. Rice treated with filtrate fermentation broth exhibited multiple defense mechanisms in response, including the enhanced expression of disease process-related genes and transcription factor genes, and significantly upregulated the gene expression of titin, salicylic acid pathway-related genes, and H2O2 accumulation, in plants; this may directly or indirectly act as an antagonist to pathogenic infestation. Further analysis revealed that the n-butanol crude extract of Bacillus mojavensis UTF-33 could retard or even inhibit conidial germination and prevent the formation of adherent cells both in vitro and in vivo. In addition, the amplification of functional genes for biocontrol using specific primers showed that Bacillus mojavensis UTF-33 expresses genes that can direct the synthesis of bioA, bmyB, fenB, ituD, srfAA and other substances; this information can help us to determine the extraction direction and purification method for inhibitory substances at a later stage. In conclusion, this is the first study to identify Bacillus mojavensis as a potential agent for the control of rice diseases; this strain, and its bioactive substances, have the potential to be developed as biopesticides.

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“…Hydrolytic enzymes secreted by bacteria are directly involved in the lysis of phytopathogenic fungi [ 64 ]. The abilities of B. mojavensis strains to secrete plant growth modulators are well documented [ 21 , 24 , 26 ]. Since phenotypic variation leads to reversible genomic alteration, it was reasonable to investigate whether it induces changes in the expression (up-expression or low-expression) of secondary metabolites and hydrolytic enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…This phenomenon might have been due to the down-expression of genes involved in IAA and hydrolytic enzyme biosynthesis. B. mojavensis PS17(I) showed the biocontrol characteristics of the original strain B. mojavensis PS17 [ 26 ]. In this case, the mechanisms for phase variation might have inactivated or caused a genetic deletion in the gene clusters or genes responsible for the expression of these metabolites.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Phenotypic Variation on Biological Properties of Endophytic Bacteria Bacillus mojavensis PS17

Diabankana

Validov

Vyshtakalyuk

et al. 2022

Biology

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The use of microorganism-based products in agricultural practices is gaining more interest as an alternative to chemical methods due to their non-toxic bactericidal and fungicidal properties. Various factors influence the efficacy of the microorganisms used as biological control agents in infield conditions as compared to laboratory conditions due to ecological and physiological aspects. Abiotic factors have been shown to trigger phase variations in bacterial microorganisms as a mechanism for adapting to hostile environments. In this study, we investigated the stability of the morphotype and the effects of phenotypic variation on the biological properties of Bacillus mojavensis strain PS17. B. mojavensis PS17 generated two variants (opaque and translucent) that were given the names morphotype I and II, respectively. The partial sequence of the 16S rRNA gene revealed that both morphotypes belonged to B. mojavensis. BOX and ERIC fingerprinting PCR also showed the same DNA profiles in both morphotypes. The characteristics of morphotype I did not differ from the original strain, while morphotype II showed a lower hydrolytic enzyme activity, phytohormone production, and antagonistic ability against phytopathogenic fungi. Both morphotypes demonstrated endophytic ability in tomato plants. A low growth rate of the strain PS17(II) in a minimal medium was observed in comparison to the PS17(I) strain. Furthermore, the capacity for biocontrol of B. mojavensis PS17(II) was not effective in the suppression of root rot disease in the tomato plants caused by Fusarium oxysporum f. sp. radices-lycopersici stain ZUM2407, compared to B. mojavensis PS17(I), whose inhibition was almost 47.9 ± 1.03% effective.

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Antifungal Properties, Abiotic Stress Resistance, and Biocontrol Ability of Bacillus mojavensis PS17

Cited by 23 publications

References 42 publications

Journal of Plant Protection Research

Journal of Plant Protection Research

Antifungal mechanisms of the antagonistic bacterium Bacillus mojavensis UTF-33 and its potential as a new biopesticide

Effects of Phenotypic Variation on Biological Properties of Endophytic Bacteria Bacillus mojavensis PS17

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