Antifungal potential of isoespintanol extracted from Oxandra xylopioides diels (Annonaceae) against intrahospital isolations of Candida SPP

Martínez, Orfa Inés Contreras; Ortíz, Alberto Angulo; Patiño, Gilmar Santafé

doi:10.1016/j.heliyon.2022.e11110

Cited by 8 publications

(7 citation statements)

References 36 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In response to this pressing clinical challenge, compounds derived from botanical sources have gained traction as a promising alternative [23][24][25]. In our previous investigations, we established that isoespintanol (ISO), a monoterpene derived from Oxandra xylopioides, exhibits potent antifungal properties against Candida tropicalis [26,27]. Our findings elucidated ISO's ability to induce intracellular reactive oxygen species, disrupt cell membranes, and eradicate mature biofilms as part of its multifaceted antifungal action.…”

Section: Introductionmentioning

confidence: 82%

“…In prior studies, we established the efficacy of isoespintanol (ISO) against clinical Candida spp. isolates, including C. tropicalis, and elucidated its molecular targets [26,27,84]. In this study, we employ a transcriptomic approach to delineate gene expression profiles, providing insights into the intricate mechanisms underlying ISO's antifungal activity.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional Reprogramming of Candida tropicalis in Response to Isoespintanol Treatment

Contreras-Martínez,

Angulo-Ortíz,

Santafé-Patiño

et al. 2023

JoF

View full text Add to dashboard Cite

Candida tropicalis, an opportunistic pathogen, ranks among the primary culprits of invasive candidiasis, a condition notorious for its resistance to conventional antifungal drugs. The urgency to combat these drug-resistant infections has spurred the quest for novel therapeutic compounds, with a particular focus on those of natural origin. In this study, we set out to evaluate the impact of isoespintanol (ISO), a monoterpene derived from Oxandra xylopioides, on the transcriptome of C. tropicalis. Leveraging transcriptomics, our research aimed to unravel the intricate transcriptional changes induced by ISO within this pathogen. Our differential gene expression analysis unveiled 186 differentially expressed genes (DEGs) in response to ISO, with a striking 85% of these genes experiencing upregulation. These findings shed light on the multifaceted nature of ISO’s influence on C. tropicalis, spanning a spectrum of physiological, structural, and metabolic adaptations. The upregulated DEGs predominantly pertained to crucial processes, including ergosterol biosynthesis, protein folding, response to DNA damage, cell wall integrity, mitochondrial activity modulation, and cellular responses to organic compounds. Simultaneously, 27 genes were observed to be repressed, affecting functions such as cytoplasmic translation, DNA damage checkpoints, membrane proteins, and metabolic pathways like trans-methylation, trans-sulfuration, and trans-propylamine. These results underscore the complexity of ISO’s antifungal mechanism, suggesting that it targets multiple vital pathways within C. tropicalis. Such complexity potentially reduces the likelihood of the pathogen developing rapid resistance to ISO, making it an attractive candidate for further exploration as a therapeutic agent. In conclusion, our study provides a comprehensive overview of the transcriptional responses of C. tropicalis to ISO exposure. The identified molecular targets and pathways offer promising avenues for future research and the development of innovative antifungal therapies to combat infections caused by this pathogenic yeast.

show abstract

Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Transcriptional Reprogramming of Candida tropicalis in Response to Isoespintanol Treatment

Contreras-Martínez,

Angulo-Ortíz,

Santafé-Patiño

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

“…Consistent with our previous work, where we reported its ability to inhibit mature biofilms in C. tropicalis [ 19 ], with inhibition percentages between 20.3% and 25.8% higher than the percentages shown with AFB (7.2% and 12.4%). Moreover, the effect of ISO on the mature biofilms of other Candida species has been reported, even in those isolates where AFB did not show an effect [ 18 ]. Our results show percentages of inhibition in the formation of biofilms by ISO greater than 50% (between 59.18% and 89.35), higher than those shown by AFB.…”

Section: Discussionmentioning

confidence: 99%

“…The biological potential of this compound as a natural antioxidant [ 10 ], anti-inflammatory [ 9 ], antispasmodic [ 11 ], vasodilator [ 12 ], cardioprotective [ 13 ], and cryoprotectant in canine semen [ 14 ], as well as its insecticidal activity [ 15 ] and antifungal activity against phytopathogens of the genus Colletotrichum [ 16 ], has been documented. We have also reported its potential against human pathogens, specifically intra-hospital bacteria [ 17 ] and yeasts of the genus Candida , reporting its action against the cell membrane, its ability to induce intracellular reactive oxygen species, and its ability to eradicate mature biofilms as responsible for its antifungal activity [ 18 , 19 ]. Continuing with the study of this compound, we hypothesize that ISO may have other targets of action against C. tropicalis .…”

Section: Introductionmentioning

confidence: 99%

Isoespintanol Antifungal Activity Involves Mitochondrial Dysfunction, Inhibition of Biofilm Formation, and Damage to Cell Wall Integrity in Candida tropicalis

Contreras Martínez,

Angulo Ortíz,

Santafé Patiño

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

The growing increase in infections caused by C. tropicalis, associated with its drug resistance and consequent high mortality, especially in immunosuppressed people, today generates a serious global public health problem. In the search for new potential drug candidates that can be used as treatments or adjuvants in the control of infections by these pathogenic yeasts, the objective of this research was to evaluate the action of isoespintanol (ISO) against the formation of fungal biofilms, the mitochondrial membrane potential (ΔΨm), and its effect on the integrity of the cell wall. We report the ability of ISO to inhibit the formation of biofilms by up to 89.35%, in all cases higher than the values expressed by amphotericin B (AFB). Flow cytometric experiments using rhodamine 123 (Rh123) showed the ability of ISO to cause mitochondrial dysfunction in these cells. Likewise, experiments using calcofluor white (CFW) and analyzed by flow cytometry showed the ability of ISO to affect the integrity of the cell wall by stimulating chitin synthesis; these changes in the integrity of the wall were also observed through transmission electron microscopy (TEM). These mechanisms are involved in the antifungal action of this monoterpene.

show abstract

“…Chemical and biological activities such as antioxidant [19], anti-inflammatory [20], antispasmodic [21], vasodilator [22] and cryoprotective of canine semen, has been reported for this compound [23], as well as insecticidal [24] and antifungal activities against Colletotrichum [25]. Furthermore, its activity against human pathogenic bacteria and yeasts has also been documented [26][27][28][29]. The cytotoxicity of this compound has been studied in the line of immortalized African green monkey kidney epithelial cells (VERO) [28], human peripheral blood lymphocytes [30] and murine macrophages (RAW 264.7) [31].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Potential of the Monoterpene Isoespintanol against Human Tumor Cell Lines

Contreras-Martínez,

Angulo-Ortíz,

Santafé Patiño

et al. 2024

Preprint

View full text Add to dashboard Cite

Cancer is a disease that encompasses multiple and different malignant conditions and is among the leading causes of death in the world. Therefore, the search for new pharmacotherapeutic options and potential candidates that can be used as treatments or adjuvants to control this disease is urgent. Natural products, especially those obtained from plants, have played an important role as a source of specialized metabolites with recognized pharmacological properties against cancer, therefore, they are an excellent alternative to be used. The objective of this research was to evaluate the action of the monoterpene isoespintanol (ISO) against the human tumor cell lines MDA-MB-231, A549, DU145, A2780, A2780-cis and the non-tumor line MRC-5. Experiments with MTT and fluorescence with propidium iodide (PI), DAPI and green plasma revealed the cytotoxicity of ISO against these cells; furthermore, morphological and chromogenic studies revealed the action of ISO on cell morphology and the inhibitory capacity on reproductive viability to form colonies in MDA-MB-231 cells. Likewise, 3D experiments validated the damage in these cells caused by this monoterpene. These results serve as a basis for progress in studies of the mechanisms of action of these compounds and the development of derivatives or synthetic analogues with a better antitumor profile.

show abstract

Antifungal potential of isoespintanol extracted from Oxandra xylopioides diels (Annonaceae) against intrahospital isolations of Candida SPP

Cited by 8 publications

References 36 publications

Transcriptional Reprogramming of Candida tropicalis in Response to Isoespintanol Treatment

Transcriptional Reprogramming of Candida tropicalis in Response to Isoespintanol Treatment

Isoespintanol Antifungal Activity Involves Mitochondrial Dysfunction, Inhibition of Biofilm Formation, and Damage to Cell Wall Integrity in Candida tropicalis

Cytotoxic Potential of the Monoterpene Isoespintanol against Human Tumor Cell Lines

Contact Info

Product

Resources

About