Antifungal, phytotoxic and hemagglutination activity of methanolic extracts of Ocimum basilicum

Ahmad, Khalil; Khalil, Ali Talha; Yusra, -; Somayya, Ramla

doi:10.1016/s0254-6272(17)30017-1

Cited by 34 publications

(22 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Linear mycelial growth inhibition assay was used as described previously [31]. Sabouraud Dextrose Liquid medium (Oxoid CMO147) supplemented with 1.5% agar technical (Oxoid LP0012) was used to prepare fungal growth media.…”

Section: Antifungal Activitymentioning

confidence: 99%

Sageretia thea (Osbeck.) modulated biosynthesis of NiO nanoparticles and their in vitro pharmacognostic, antioxidant and cytotoxic potential

Khalil

Ovais

Ullah

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

166

View full text Add to dashboard Cite

NiO nanoparticles are biosynthesized using Sageretia thea (Osbeck.) aqueous leave extracts and their biological activities are reported. Nanoparticles (∼18 nm) were characterized through XRD, ATR-FTIR, EDS, SAED, HR-SEM/TEM and Raman spectroscopy. Antibacterial activity was investigated against six pathogenic bacterial strains (gram positive and gram negative) and their corresponding minimum inhibitory concentrations (MICs) were calculated. UV-exposed nanoparticles were investigated to have reduced MICs relative to the NiO nanoparticles have not been exposed to UV. Moderate linear fungal growth inhibition was observed while Mucor racemosus (percentage inhibition 64% ± 2.30) was found to be most susceptible. Cytotoxicity was confirmed using brine shrimps lethality assay (IC 42.60 μg/ml). MTT cytotoxicity was performed against Leishmania tropica-KWH23 promastigotes and amastigotes revealed significant percentage inhibition across the applied concentrations. IC values were calculated as 24.13 μg/ml and 26.74 μg/ml for the promastigote and amastigote cultures of Leishmania tropica. NiO nanoparticles were found. Moderate, antioxidant potential was concluded through assays like DPPH, TAP and TAC. Furthermore, protein kinase inhibition and alpha amylase inhibition is also reported.

show abstract

Section: Antifungal Activitymentioning

confidence: 99%

Sageretia thea (Osbeck.) modulated biosynthesis of NiO nanoparticles and their in vitro pharmacognostic, antioxidant and cytotoxic potential

Khalil

Ovais

Ullah

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

166

View full text Add to dashboard Cite

show abstract

“…Antifungal activity of test samples was determined using linear mycelial growth inhibition (38) against Candida albicans, Fusarium oxysporum, Aspergillus flavus, Aspergillus parasiticus, Penicillium digitatum, and Aspergillus niger. SDA media was prepared and autoclaved.…”

Section: Antifungal Activitymentioning

confidence: 99%

Daphne mucronata-mediated phytosynthesis of silver nanoparticles and their novel biological applications, compatibility and toxicity studies

Shah

Lutfullah

Ahmad

et al. 2018

Green Chemistry Letters and Reviews

Self Cite

View full text Add to dashboard Cite

show abstract

“…The presence of various bioactive components in different parts of plants make them an important resource for the treatment of various diseases. Furthermore, the side effects associated with some of the synthetic medications and antibiotic resistance demands intermittent research for alternative solutions [ 2 , 3 ]. In addition, to their direct therapeutic use, medicinal plants have been explored to fabricate various nanoscaled materials for potential biomedical applications [ 4 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana

Ovais

Ayaz

Khalil³

et al. 2018

BMC Complement Altern Med

Self Cite

201

159

View full text Add to dashboard Cite

BackgroundThe medicinal importance of a novel plant Olax nana Wall. ex Benth. (family: Olacaceae) was revealed for the first time via HPLC-DAD finger printing, qualitative phytochemical analysis, antioxidant, cholinesterase, and α-glucosidase inhibitory assays.MethodsThe crude methanolic extract of O. nana (ON-Cr) was subjected to qualitative phytochemical analysis and HPLC-DAD finger printing. The antioxidant potential of ON-Cr was assessed via 1,1-diphenyl,2-picrylhydrazyl (DPPH), 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) and hydrogen peroxide (H2O2) free radical scavenging assays. Furthermore, acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities were performed using Ellman’s assay, while α- glucosidase inhibitory assay was carried out using a standard protocol.ResultsThe qualitative phytochemical analysis of ON-Cr revealed the presence of secondary metabolites like alkaloids, flavonoids, tannins, sterols, saponins and terpenoids. The HPLC-DAD finger printing revealed the presence of 40 potential compounds in ON-Cr. Considerable anti-radical activities was revealed by ON-Cr in the DPPH, ABTS and H2O2 free radical scavenging assays with IC50 values of 71.46, 72.55 and 92.33 μg/mL, respectively. Furthermore, ON-Cr showed potent AChE and BChE inhibitory potentials as indicated by their IC50 values of 33.2 and 55.36 μg/mL, respectively. In the α-glucosidase inhibition assay, ON-Cr exhibited moderate inhibitory propensity with an IC50 value of 639.89 μg/mL.ConclusionsThis study investigated Olax nana for the first time for detailed qualitative phytochemical tests, HPLC-DAD finger printing analysis, antioxidant, anticholinesterase and α-glucosidase inhibition assays. The antioxidant and cholinesterase inhibitory results were considerable and can provide scientific basis for further studies on the neuroprotective and anti-Alzheimer’s potentials of this plant. ON-Cr may further be subjected to fractionation and polarity guided fractionation to narrow down the search for isolation of bioactive compounds.

show abstract

Antifungal, phytotoxic and hemagglutination activity of methanolic extracts of Ocimum basilicum

Cited by 34 publications

References 28 publications

Sageretia thea (Osbeck.) modulated biosynthesis of NiO nanoparticles and their in vitro pharmacognostic, antioxidant and cytotoxic potential

Sageretia thea (Osbeck.) modulated biosynthesis of NiO nanoparticles and their in vitro pharmacognostic, antioxidant and cytotoxic potential

Daphne mucronata-mediated phytosynthesis of silver nanoparticles and their novel biological applications, compatibility and toxicity studies

HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana

Contact Info

Product

Resources

About