Antifungal metabolites produced by Pseudomonas fluorescens against Fusarium oxysporum f.sp. cepae

Malathi, S.

doi:10.15740/has/ijpp/8.2/324-330

Cited by 2 publications

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“…Earlier, antifungal metabolites, i.e Phenazine and 2,4 DAPG extracted from Pseudomonas fluorescens recorded 67.03 and 77.34 per cent inhibition of mycelial growth of Fusarium oxysporum f.sp. cepae causing basal rot of onion respectively (Malathi 2015). P. putida WCS 358r produced phenazine-3-carboxylic acid (PCA) and 2, 4 DAPG which has antifungal and antibacterial action (Bakker et al 2002).…”

Section: Resultsmentioning

confidence: 99%

Characterization of secondary metabolites produced during interaction of Pseudomonas fluorescens with Fusarium oxysporum

Sharma

Gupta

et al. 2019

Indian J Agri Sci

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Characterization of the Secondary Metabolites (SM) produced by Pseudomonas fluorescens (NAIMCC-B-00361) while growing in mixed culture conditions with fungal pathogen Fusarium oxysporum (NAIMCC-F-00811) was done to better mimic antagonism and interaction in a natural environment. Nutrient media, viz. Kingâ€™s B Broth (KBB), Potato Dextrose Broth (PDB), Pigment Production Medium (PPM), Czapek Dox broth Medium (CDB) and half minimal media (50% KBB and 50% PDB) were assessed and KBB was found to be the best medium for the production of these metabolites. Antifungal assay of crude metabolite extract was done using poison food technique and the results showed that the crude chloroform extract of metabolites (mixed culture of P. fluorescens and F. oxysporum) grown in KBB medium showed 50.47% inhibition of mycelial growth of F. oxysporum followed by the chloroform extract of solo culture of P. fluorescens which showed 45.38% inhibition of mycelial growth of Bipolaris oryzae. Antibacterial assay of crude metabolite extract was done using Agar well diffusion technique and the results revealed that the crude chloroform extract of mixed culture of P. fluorescens and F. oxysporum grown in KBB medium showed 25.67 mm zone of inhibition against Bacillus subtilis, whereas the extract of solo culture of P. fluorescens showed 18.67 mm zone of inhibition against Klebsella pneumoniae. The qualitative confirmation of antibiotic production by solo culture of P. fluorescens and mixed culture using TLC revealed the presence of antibiotics, i.e. 2, 4-DAPG, pyrrolnitrin and phenazine. HPLC analysis of crude chloroform extract of SM produced by solo culture of P. fluorescens and mixed culture showed the presence of 2,4-DAPG at retention time 20.707 and 20.698 respectively.

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Section: Resultsmentioning

confidence: 99%

Characterization of secondary metabolites produced during interaction of Pseudomonas fluorescens with Fusarium oxysporum

Sharma

Gupta

et al. 2019

Indian J Agri Sci

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“…The typical identification procedures based on motility, shape, colony color, spore formation, fluorescence, Gram staining properties, and other biochemical tests assumed that the isolated bacterium was P. aeruginosa, as previously described (Abd-Alla et al, 2011;Mahmoud et al, 2016). Pseudomonas aeruginosa has a uniquely distinctive character: the secretion of a pyocyanin pigment (Garrity et al, 2005) which is a water-soluble blue-green phenazine compound that may have antimicrobial activity and exhibit fluorescence on KB medium under UV light.…”

Section: Discussionmentioning

confidence: 99%

“…Pseudomonas aeruginosa has a uniquely distinctive character: the secretion of a pyocyanin pigment (Garrity et al, 2005) which is a water-soluble blue-green phenazine compound that may have antimicrobial activity and exhibit fluorescence on KB medium under UV light. Pyocyanin biosynthesis, purification, and characterization were reported by Mavrodi et al (2001) and Mahmoud et al (2016).…”

Section: Discussionmentioning

confidence: 99%

First Report: Metabolic and Molecular Evidence for the Detection of the Pathogenic Pseudomonas aeruginosa on Common Bean Seeds and Its Control Via Chitosan-silver Nanocomposite

Fouad,

Saber,

Badr

et al. 2023

Egypt. J. Bot.

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T HE CURRENT study stated the novel pathogenic action of Pseudomonas aeruginosa MA1 on common bean, causing the soft rot of seeds as the infection route. The pathogenic bacterium was molecularly identified and deposited in NCBI GenBank with an accession number of ON000399. Enzymatic activities of xylanase, cellulase, pectinase, proteinase and protease were observed to be 13.054, 11.09, 9.28, 20.90, and 1.47U respectively, indicating the ability of MA1 to invade the plant tissue, recording a disease incidence of 63.25% with a severity of 42.52%. The physiological features showed a remarkable decrease in chlorophyll content with an increase in proline and phenolic contents in the infected plant. Both peroxidase and phenylalanine ammonia-lyase (PAL) activities increased, reaching their maximum value after 72h of infection with a significant overexpression of the PAL gene after 24h. that proves the pathogenic role of P. aeruginosa on common bean. However, seed transmission is paramount in further pathological studies since the current pathogenic bacterium was detected on seeds. In-vitro evaluation of the chitosan-silver nanocomposite (Ch-Ag NC) reveals the first management protocol for the bacterial pathogen MA1 that was confirmed by applying (Ch-Ag NC) in the greenhouse. Furthermore, in-situ application of Ch-Ag NC (50 and 100mg/L) suppressed bacterial infection with a significant increase in seedling growth properties.

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Antifungal metabolites produced by Pseudomonas fluorescens against Fusarium oxysporum f.sp. cepae

Cited by 2 publications

References 0 publications

Characterization of secondary metabolites produced during interaction of Pseudomonas fluorescens with Fusarium oxysporum

Characterization of secondary metabolites produced during interaction of Pseudomonas fluorescens with Fusarium oxysporum

First Report: Metabolic and Molecular Evidence for the Detection of the Pathogenic Pseudomonas aeruginosa on Common Bean Seeds and Its Control Via Chitosan-silver Nanocomposite

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