Antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum against different oral Candida species isolated from HIV/ AIDS patients: an in vitro study

Salari, Samira; Almani, Pooya Ghasemi Nejad

doi:10.1080/20002297.2020.1769386

Cited by 25 publications

(18 citation statements)

References 34 publications

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“…In this study, we investigated the growth inhibition of 14 selected Lactobacillus strains isolated from the human oral cavity or vagina. We found a clear but strain-dependent effect on Candida growth which was in harmony with several previous in vitro experiments [9,17,26,28,29]. Interestingly, three of the best performing strains were L. rhamnosus strains, and the two best growth inhibitors, L. rhamnosus DSM 32992 and L. rhamnosus DSM 32991, were isolated from the oral cavity.…”

Section: Discussionsupporting

confidence: 90%

Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro

Jørgensen

Rikvold

Lichtenberg

et al. 2020

Journal of Oral Microbiology

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Background: Intake of probiotic bacteria may prevent oral Candida infection. Objective: To screen the antifungal activity of 14 Lactobacillus candidate strains of human origin, against six opportunistic C. albicans and non-albicans species. A second aim was to study the acid production of the four strains showing the strongest antifungal activity. Methods: We used an agar overlay growth inhibition assay to the assess the antifungal activity of the lactobacilli. The acid-producing capacity was measured with pH micro-sensors. Results: All 14 Lactobacillus candidates inhibited the growth of the Candida spp. The four best-performing strains were L. rhamnosus DSM 32992 (oral origin), L. rhamnosus DSM 32991 (oral), L. jensenii 22B42 (vaginal), and L. rhamnosus PB01 (vaginal). The difference between L. rhamnosus DSM 32992 and the other three strains was statistically significant (p < 0.001). The Candida spp. differed in susceptibility; C. parapsilosis was highly inhibited, while C. krusei was not or slightly inhibited. The oral L. rhamnosus DSM 32992 and DSM 32991 strains showed the lowest pH-values. Conclusion: Screening of probiotic lactobacilli showed significant strain-dependent variations in their antifungal capacity in a pH-dependent mode. Two strains of oral origin were most effective. A further characterization seems justified to elaborate on their probiotic properties.

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Section: Discussionsupporting

confidence: 90%

Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro

Jørgensen

Rikvold

Lichtenberg

et al. 2020

Journal of Oral Microbiology

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“…Candidiasis is the major common invasive fungal infection in human. 8 Due to the side effects of antifungal drugs and the F I G U R E 3 X-ray diffraction pattern obtained from TiO 2 -NPs F I G U R E 4 X-ray diffraction pattern obtained from ZnO-NPs development of resistant fungal species and therapeutic failures, attention has been drawn to the use of novel antifungal compounds with fewer side effects. 18 One of these novel compounds is na- nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…2 are recommended. 7,8 A major concern of the increasing consumption of chemical drugs like antifungal agents is their side effects, which might in some cases be even more dangerous than the disease itself. Headache, nausea, vomiting, hepatotoxicity, and anaphylaxis are some side effects of these drugs.…”

Section: Introductionmentioning

confidence: 99%

Comparison of antifungal and cytotoxicity activities of titanium dioxide and zinc oxide nanoparticles with amphotericin B against different Candida species: In vitro evaluation

Kermani¹,

Salari

Almani

2020

Clinical Laboratory Analysis

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“…C albicans is the major species in the development of oral candidiasis. Other Candida species including C glabrata, C kefyr, C tropicalis, C parapsilosis, C krusei, and C guilliermondii are found in different parts of the oral cavity 6 . The frequency of Candida species in the oral cavity was 40%‐60% in healthy subjects, 8 while it was between 62% and 93% in the HIV/ AIDS patients 9…”

Section: Introductionmentioning

confidence: 99%

“…Many people with OC can stay without any specific clinical symptoms for a long time. However, several symptoms in these people may include burning sensation and pain in the mouth, changes in taste sensation, and difficulty in swallowing liquids or solids, and or white creamy or creamy plaques in different parts of the oral cavity 6,7 …”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Candida isolates by Real‐time PCR‐high‐resolution melting analysis and investigation of the genetic diversity of Candida species

Nejad

Almani

Mohammadi

et al. 2020

Clinical Laboratory Analysis

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Background Candida species are considered as the cause of one of the most important opportunistic fungal diseases. Accurate identification of Candida species is important because of antifungal susceptibility patterns are different among these species, so proper identification helps in the selection of antifungal drugs for the prevention and treatment. Phenotypic methods for identification of Candida species, which are widely used in clinical microbiology laboratories, have some limitations. Real‐time PCR followed by the high‐resolution melting analysis (HRMA) is a novel approach for the rapid recognition of pathogenic fungi. Molecular phylogeny is essential for obtaining a better understanding of the evolution of the genus Candida and the identification of the relative degree of the Candida species. The purpose of this study was molecular identification of Candida isolates by Real‐time PCR‐high‐resolution melting analysis and investigation of the genetic diversity of Candida species. Methods Two hundred and thirty‐two Candida isolates including 111 Candida isolates obtained from 96 HIV/AIDS patients and 121 Candida isolates obtained from 98 non‐HIV persons were identified by real‐time PCR and high‐resolution melting curve analysis. To evaluate genetic diversity and relationships among Candida species, PCR products of nine clinical Candida isolates, as a representative of each kind of species, were randomly selected for DNA sequence analysis. Results In HIV/AIDS patients, six species of Candida spp. were identified as follows: C albicans (n = 64; 57.7%), C glabrata (n = 31; 27.92%), C parapsilosis (n = 9; 8.1%), C tropicalis (n = 4; 3.6%), C krusei (n = 2; 1.8%), and C kefyr (n = 1; 0.90%). In non‐HIV persons, we identified eight species of Candida including C albicans (n = 46; 38.33%) followed by C glabrata and C krusei (each one, n = 18; 15%), C tropicalis (n = 13; 10.83%), C lusitaniae (n = 12; 5.17%), C parapsilosis (n = 10; 4.31%), and C kefyr and C guillermondii (each one, n = 2; 1.66%). Also, the phylogenetic analysis showed the presence of two main clades and six separate subclades. Accordingly, about 88.9% of the isolates were located in clade I and 11.10% of the studied isolates were in clade II. Conclusions Real‐time PCR followed by high‐resolution melting analysis (HRMA) is known as a reliable, fast, and simple approach for detection and accurate identification of Candida species, especially in clinical samples.

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Antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum against different oral Candida species isolated from HIV/ AIDS patients: an in vitro study

Cited by 25 publications

References 34 publications

Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro

Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro

Comparison of antifungal and cytotoxicity activities of titanium dioxide and zinc oxide nanoparticles with amphotericin B against different Candida species: In vitro evaluation

Molecular identification of Candida isolates by Real‐time PCR‐high‐resolution melting analysis and investigation of the genetic diversity of Candida species

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