Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense

Wang, Xiaoxia; Du, Zhenghua; Chen, Chanxin; Guo, Song; Mao, Qianzhuo; Wu, Wei; Wu, Ruimei; Han, Wenbo; Xie, Peifeng; Zeng, Yiping; Shan, Wenna; Wang, Zonghua; Yu, Xiaomin

doi:10.3389/fmicb.2023.1177393

Cited by 5 publications

(2 citation statements)

References 56 publications

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“…produce various cyclic lipopeptides (CLPs), polyketides, dipeptides, RiPPs and volatile organic compounds ( Arguelles Arias et al., 2013 ; Caulier et al., 2019 ; Anckaert et al., 2021 ). Streptomyces species are known producers of several antibiotics such as kasugamycin (antifungal and antibacterial), polyoxin B and D, validamycin (antifungal) families ( Barka et al., 2016 ), as well as cyclic lipopeptides such as lipopeptins recently discovered with antagonistic activity against Fusarium oxysporum ( Wang et al., 2023 ). Paenibacillus spp.…”

Section: Plant Growth-promoting Rhizobacteria In Crop Protectionmentioning

confidence: 99%

Bacillus-based biocontrol beyond chemical control in central Africa: the challenge of turning myth into reality

Nihorimbere,

Korangi Alleluya,

Nimbeshaho

et al. 2024

Front. Plant Sci.

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Agricultural productivity in the Great Lakes Countries of Central Africa, including Burundi, Rwanda, and the Democratic Republic of Congo, is affected by a wide range of diseases and pests which are mainly controlled by chemical pesticides. However, more than 30% of the pesticides used in the region are banned in European Union due to their high toxicity. Globally available safe and eco-friendly biological alternatives to chemicals are virtually non-existent in the region. Bacillus PGPR-based biocontrol products are the most dominant in the market and have proven their efficacy in controlling major plant diseases reported in the region. With this review, we present the current situation of disease and pest management and urge the need to utilize Bacillus-based control as a possible sustainable alternative to chemical pesticides. A repertoire of strains from the Bacillus subtilis group that have shown great potential to antagonize local pathogens is provided, and efforts to promote their use, as well as the search for indigenous and more adapted Bacillus strains to local agro-ecological conditions, should be undertaken to make sustainable agriculture a reality in the region.

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Section: Plant Growth-promoting Rhizobacteria In Crop Protectionmentioning

confidence: 99%

Bacillus-based biocontrol beyond chemical control in central Africa: the challenge of turning myth into reality

Nihorimbere,

Korangi Alleluya,

Nimbeshaho

et al. 2024

Front. Plant Sci.

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“…XY006 lowered the synthesis of fusaric acid in Fusarium oxysporum f. sp. cubense ( Wang et al., 2023 ).…”

Section: Biocontrol Agents Against Fusarium and Th...mentioning

confidence: 99%

Microbial diversity in soils suppressive to Fusarium diseases

Todorović,

Moënne-Loccoz,

Raičević

et al. 2023

Front. Plant Sci.

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Fusarium species are cosmopolitan soil phytopathogens from the division Ascomycota, which produce mycotoxins and cause significant economic losses of crop plants. However, soils suppressive to Fusarium diseases are known to occur, and recent knowledge on microbial diversity in these soils has shed new lights on phytoprotection effects. In this review, we synthesize current knowledge on soils suppressive to Fusarium diseases and the role of their rhizosphere microbiota in phytoprotection. This is an important issue, as disease does not develop significantly in suppressive soils even though pathogenic Fusarium and susceptible host plant are present, and weather conditions are suitable for disease. Soils suppressive to Fusarium diseases are documented in different regions of the world. They contain biocontrol microorganisms, which act by inducing plants’ resistance to the pathogen, competing with or inhibiting the pathogen, or parasitizing the pathogen. In particular, some of the Bacillus, Pseudomonas, Paenibacillus and Streptomyces species are involved in plant protection from Fusarium diseases. Besides specific bacterial populations involved in disease suppression, next-generation sequencing and ecological networks have largely contributed to the understanding of microbial communities in soils suppressive or not to Fusarium diseases, revealing different microbial community patterns and differences for a notable number of taxa, according to the Fusarium pathosystem, the host plant and the origin of the soil. Agricultural practices can significantly influence soil suppressiveness to Fusarium diseases by influencing soil microbiota ecology. Research on microbial modes of action and diversity in suppressive soils should help guide the development of effective farming practices for Fusarium disease management in sustainable agriculture.

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Antifungal potential of lipopeptides produced by the Bacillus siamensis Sh420 strain against Fusarium graminearum

Hussain,

Tai,

Ali

et al. 2024

Microbiol Spectr

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Biological control is a more sustainable and environmentally friendly alternative to chemical fungicides for controlling Fusarium spp. infestations. In this work, Bacillus siamensis Sh420 isolated from wheat rhizosphere showed a high antifungal activity against Fusarium graminearum as a secure substitute for fungicides. Sh420 was identified as B. siamensis using phenotypic evaluation and 16S rDNA gene sequence analysis. An in vitro antagonistic study showed that Sh420’s lipopeptide (LP) extract exhibited strong antifungal properties and effectively combated F. graminearum . Meanwhile, lipopeptides have the ability to decrease ergosterol content, which has an impact on the overall structure and stability of the plasma membrane. The PCR-based screening revealed the presence of antifungal LP biosynthetic genes in this strain’s genomic DNA. In the crude LP extract of Sh420, we were able to discover several LPs such as bacillomycin, iturins, fengycin, and surfactins using ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry. Microscopic investigations (fluorescent/transmission electron microscopy) revealed deformities and alterations in the morphology of the phytopathogen upon interaction with LPs. Sh420 LPs have been shown in grape tests to be effective against F. graminearum infection and to stimulate antioxidant activity in fruits by avoiding rust and gray lesions. The overall findings of this study highlight the potential of Sh420 lipopeptides as an effective biological control agent against F. graminearum infestations. IMPORTANCE This study addresses the potential of lipopeptide (LP) extracts obtained from the strain identified as Bacillus siamensis Sh420. This Sh420 isolate acts as a crucial player in providing a sustainable and environmentally friendly alternative to chemical fungicides for suppressing Fusarium graminearum phytopathogen. Moreover, these LPs can reduce ergosterol content in the phytopathogen influencing the overall structure and stability of its plasma membrane. PCR screening provided confirmation regarding the existence of genes responsible for biosynthesizing antifungal LPs in the genomic DNA of Sh420. Several antibiotic lipopeptide compounds were identified from this bacterial crude extract using ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry. Microscopic investigations revealed deformities and alterations in the morphology of F. graminearum upon interaction with LPs. Furthermore, studies on fruit demonstrated the efficacy of Sh420 LPs in mitigating F. graminearum infection and stimulating antioxidant activity in fruits, preventing rust and gray lesions.

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Antifungal effects and biocontrol potential of lipopeptide-producing Streptomyces against banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense

Cited by 5 publications

References 56 publications

Bacillus-based biocontrol beyond chemical control in central Africa: the challenge of turning myth into reality

Bacillus-based biocontrol beyond chemical control in central Africa: the challenge of turning myth into reality

Microbial diversity in soils suppressive to Fusarium diseases

Antifungal potential of lipopeptides produced by the Bacillus siamensis Sh420 strain against Fusarium graminearum

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