Antifungal effect of a new photosensitizer derived from BODIPY on Candida albicans biofilms

Wang, Mengran; Gu, Kedan; Ding, Wenxin; Wan, Miyang; Zhao, Weili; Shi, Hongcheng; Li, Jiyang

doi:10.1016/j.pdpdt.2022.102946

Cited by 3 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on our previous research on BODIPY dyes, we reported a new derivative of BODIPY named BDP-4L through installation of two cationic groups on the boron atom [25,26]. Our results suggested that BDP-4L-mediated PDI exhibited a favorable antifungal effect on C. albicans biofilms [26].…”

Section: Discussionmentioning

confidence: 79%

“…Suspensions were then inoculated in a 96-well plate (100 μL/well) at 37 °C. According to our previous research, mature biofilms were finally formed after incubation of 48 h [26].…”

Section: Cells and Culture Conditionsmentioning

confidence: 90%

“…C. albicans biofilms were cultured using resuspended fungal cells that were prepared as mentioned above and adjusted to a cell density of 2 × 10 6 CFU/mL in RPMI-1640 medium (Gibco, USA) [26]. Suspensions were then inoculated in a 96-well plate (100 μL/well) at 37 °C.…”

Section: Cells and Culture Conditionsmentioning

confidence: 99%

“…Recently, increasing attention has been focused on the photodynamic properties of BODIPY due to its large molar absorption coefficients, high fluorescence quantum yields, and tunable modifications [22][23][24]. Our previous studies reported a derivative of BODIPYs, named BDP-4L, as a promising Type II photosensitizer [25,26]. Iodinated BDP-4L carries two cationic charges on the boron atom.…”

Section: Introductionmentioning

confidence: 99%

“…It revealed good amphiphilicity, efficient ROS generation, as well as a tight interaction with the cell membrane of C. albicans [25]. More importantly, BDP-4L exhibited desirable photoinactivation efficacy on both suspension-state and biofilm-associated C. albicans in antifungal studies [25,26]. However, PDI using BDP-4L was less effective against cells in biofilms and failed to completely eliminate C. albicans biofilms.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative

Wang

Gu²,

Wan

et al. 2023

Photochem Photobiol Sci

Self Cite

View full text Add to dashboard Cite

Photodynamic inactivation (PDI) has received increasing attention as a promising approach to combat Candida albicans infections. This study aimed to evaluate the synergistic effect of a new BODIPY (4,4-difluoro-boradiazaindacene) derivative and hydrogen peroxide on C. albicans. BDP-4L in combination with H 2 O 2 demonstrated enhanced photokilling efficacy. In suspended cultures of C. albicans, the maximum decrease was 6.20 log and 2.56 log for PDI using BDP-4L (2.5 μM) with or without H 2 O 2 , respectively. For mature C. albicans biofilms, 20 μM BDP-4L plus H 2 O 2 eradicated C. albicans, causing an over 6.7 log count reduction in biofilm-associated cells, while only a reduction of ~ 1 log count was observed when H 2 O 2 was omitted. Scanning electron microscopy analysis and LIVE/DEAD assays suggested that PDI using BDP-4L plus H 2 O 2 induced more damage to the cell membrane. Correspondingly, amplification of nucleic acids release was observed in biofilms treated with the combined PDI. Additionally, we also discovered that the addition of hydrogen peroxide potentiated the generation of 1 O 2 in PDI using the singlet oxygen sensor green probe. Collectively, BDP-4L combined with H 2 O 2 presents a promising approach in the treatment of C. albicans infections.

show abstract

Section: Discussionmentioning

confidence: 79%

“…Suspensions were then inoculated in a 96-well plate (100 μL/well) at 37 °C. According to our previous research, mature biofilms were finally formed after incubation of 48 h [26].…”

Section: Cells and Culture Conditionsmentioning

confidence: 90%

Section: Cells and Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative

Wang

Gu²,

Wan

et al. 2023

Photochem Photobiol Sci

Self Cite

View full text Add to dashboard Cite

show abstract

Evaluation of 1,9-Dimethyl-Methylene Blue nanoencapsulation using rhamnolipid nanoparticles to potentiate the Photodynamic Therapy technique in Candida albicans: In vitro study

Nunes,

de Jesus,

Almeida

et al. 2024

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

Inhibitory effects of a maleimide compound on the virulence factors of Candida albicans

Chen

Liu

et al. 2023

Virulence

View full text Add to dashboard Cite

Candidiasis caused by Candida albicans infection has long been a serious human health problem. The pathogenicity of C. albicans is mainly due to its virulence factors, which are novel targets of antifungal drugs for low risk of resistance development. In this study, we identified a maleimide compound [1-(4-methoxyphenyl)-1hydro-pyrrole-2,5-dione, MPD] that exerts effective anti-virulence activity. It could inhibit the process of adhesion, filamentation, and biofilm formation in C. albicans . In addition, it exhibited low cytotoxicity, hemolytic activity, and drug resistance development. Moreover, in Galleria mellonella-C. albicans ( in vivo ) infection model, the survival time of infected larvae was significantly prolonged under the treatment of MPD. Further, mechanism research revealed that MPD increased farnesol secretion by upregulating the expression of Dpp3. The increased farnesol inhibited the activity of Cdc35, which then decreased the intracellular cAMP content resulting in the inhibition of virulence factors via the Ras1-cAMP-Efg1 pathway. In all, this study evaluated the inhibitory effect of MPD on various virulence factors of C. albicans and identified the underlying mechanisms. This suggests a potential application of MPD to overcome fungal infections in clinics.

show abstract

Antifungal effect of a new photosensitizer derived from BODIPY on Candida albicans biofilms

Cited by 3 publications

References 45 publications

Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative

Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative

Evaluation of 1,9-Dimethyl-Methylene Blue nanoencapsulation using rhamnolipid nanoparticles to potentiate the Photodynamic Therapy technique in Candida albicans: In vitro study

Inhibitory effects of a maleimide compound on the virulence factors of Candida albicans

Contact Info

Product

Resources

About