Antifungal coating by biofunctionalized polyelectrolyte multilayered films

Resistance of oxidative stress in biofilm and planktonic cells

“…The most common explanation of the bacterial biofilm durability is the mechanism of secretion of antibiotic-degrading enzymes, which reduces the possibility of penetration of the antimicrobial substances to the lower layers of cells (Stewart and Costerton 2001;Obst et al 2006;Chaignon et al 2007;Hoiby et al 2010;Banerjee et al 2011) . Another common argument is based on emerging multi-layer construction of the structure of the biofilm, which also impedes the penetration of antimicrobial substances (Etienne et al 2005;An et al 2008). Another reason for the increased resistance of biofilm cells is connected with the observation based on the presence of cells at different stages of development (Chavant et al 2004;Gad et al 2004).…”

The Natural Antimicrobial Chromogranins/Secretogranins- Derived Peptides – Production, Lytic Activity and Processing by Bacterial Proteases

“…The cytotoxicity of such a coating was also assessed by growing human gingival fibroblasts at its surface. Finally, the antifungal coating of poly (methylmethacrylate), a widely used material for biomedical devices, is successfully tested in an in vivo oral candidiasis rat model (Etienne et al, 2005).…”

Advances in polyelectrolyte multilayer nanofilms as tunable drug delivery systems

“…16 In addition, antifungal polyelectrolyte multilayer nanofilms were developed by incorporating chromogranin A-derived antifungal peptide in PLL/PLGA multilayer nanofilms, and in vitro studies demonstrated the ability of the nanofilms in inhibiting growth of the yeast Candida albicans and the filamentous fungus Neurospora crassa. 40 Polyelectrolyte multilayer nanofilms incorporated with drugs may also alleviate inflammation associated with biomedical devices. Schultz and colleagues 29 conjugated a synthetic analog of an anti-inflammatory peptide, alphamelanocyte-stimulating hormone (alpha-MSH) to PLGA, and their developed PLL/PLGA multilayer nanofilms showed reduced inflammatory response in vivo.…”