Abstract:Pyocyanin is a pigment produced by 95% of Pseudomonas aeruginosa strains and exhibits antimicrobial properties that can be used for different purposes. In this work, PMMA-based nanoparticles that were encapsulated into 200 µg/mL of pyocyanin (Np-Pyo) were produced by the nanoprecipitation method. They were evaluated with respect to antifouling activity against Aspergillus sp. and Penicillium sp. With an encapsulation efficiency of 56%, the NpPyo remained stable for 90 days. Their characteristics were satisfact… Show more
“…This study is part of a research project to characterize the strain P. aeruginosa TGC04, a wild type hydrocarbonclastic pseudomonad (29), a high pyocyanin producer (38,39,40), resistant to exposure to different wavelengths of the visible spectrum (41) and capable of using pesticides (42) and polycyclic aromatic hydrocarbons (43) as a carbon source. In addition, the characterization observed here concerns the susceptibility of P. aeruginosa TGC04 surface colonization to non-antibiotic pharmaceuticals.…”
Molecules with antimicrobial properties, such as those found in non-antibiotic pharmaceuticals, may pressure bacteria to adapt to the environment. In this work, five of the most sold non-antibiotic pharmaceuticals in Brazil and in Egypt in 2020 (losartan 50 mg, acetylsalicylic acid 100 mg, diclofenac 50 mg, paracetamol 750 mg and metformin 500 mg and dipyrone 100 mg/mL), were tested against the wild strain Pseudomonas aeruginosa TGC04, previously isolated by our group and which has been characterized over the years. We evaluated inhibition of biofilm adhesion over 48 h using the crystal violet test. Exposure of P. aeruginosa TGC04 to the compounds promoted a similar moderate reduction in adherence for all the pharmaceuticals tested, amounting to about 60%. This indicated that the cells were also moderately adherent. However, although all non-antibiotic pharmaceuticals did not produce a biocidal effect on the wild strain, intense antibiofilm activity was observed, except for paracetamol.
“…This study is part of a research project to characterize the strain P. aeruginosa TGC04, a wild type hydrocarbonclastic pseudomonad (29), a high pyocyanin producer (38,39,40), resistant to exposure to different wavelengths of the visible spectrum (41) and capable of using pesticides (42) and polycyclic aromatic hydrocarbons (43) as a carbon source. In addition, the characterization observed here concerns the susceptibility of P. aeruginosa TGC04 surface colonization to non-antibiotic pharmaceuticals.…”
Molecules with antimicrobial properties, such as those found in non-antibiotic pharmaceuticals, may pressure bacteria to adapt to the environment. In this work, five of the most sold non-antibiotic pharmaceuticals in Brazil and in Egypt in 2020 (losartan 50 mg, acetylsalicylic acid 100 mg, diclofenac 50 mg, paracetamol 750 mg and metformin 500 mg and dipyrone 100 mg/mL), were tested against the wild strain Pseudomonas aeruginosa TGC04, previously isolated by our group and which has been characterized over the years. We evaluated inhibition of biofilm adhesion over 48 h using the crystal violet test. Exposure of P. aeruginosa TGC04 to the compounds promoted a similar moderate reduction in adherence for all the pharmaceuticals tested, amounting to about 60%. This indicated that the cells were also moderately adherent. However, although all non-antibiotic pharmaceuticals did not produce a biocidal effect on the wild strain, intense antibiofilm activity was observed, except for paracetamol.
“…A aplicação industrial de antibióticos na função de biocidas é muito difundida, com destaque para a indústria alimentícia (NG et al, 2020), farmacêutica (COOK e WRIGHT, 2022), assim como de tintas e vernizes (HOQUE et al, 2015;GLADIS et al, 2010). O uso de produtos sintéticos Brazilian Journal of Development, Curitiba, v.8, n.12, p. 78646-78664, dec., 2022 causa sérios problemas ambientais, e o emprego de nanopartículas incorporadas de bioativos naturais pode ser uma opção mais sustentável (SILVA et al, 2022).…”
Os biofilmes são agregados microbianos complexos que representam risco à saúde porque inúmeros patógenos são capazes de colonizar superfícies bióticas e abióticas, conferindo a estes microrganismos, maior resistência a antibióticos e outros agentes antimicrobianos. Além disso, os biofilmes são responsáveis por perdas na indústria, bem como pelo processo de biodeterioração de diferentes materiais. Visando formas de inibir ou erradicar biofilmes, diferentes métodos são propostos, incluindo a associação entre eles. Esta revisão foi elaborada por discentes de Pós-Graduação em Biologia Celular e Molecular da UFPB, como trabalho final da Disciplina “Biofilmes microbianos” e traz exemplos e perspectivas de tratamentos físicos, químicos e biológicos no controle destas estruturas.
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