Antifungal and antioxidant properties of ten medicinal plants collected from KwaDlangezwa area, KwaZulu-Natal Province

Molele, P.K.; Mongalo, N.I.; Dikhoba, M.P.; Makhafola, Tshepiso Jan

doi:10.1016/j.sajb.2016.02.115

Cited by 2 publications

(1 citation statement)

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“…Leaf -anti-mutagenic activity, antibacterial activity, antifungal activity, antiinflammatory activity, anti-diabetic activity (Reid et al, 2006, Aderogba et al, 2007, Verchaeve & Staden, 2008, Samie et al, 2009, Ahmed et al, 2012, Farag et al,2015, and Molele et al, 2016 Bark -antibacterial activity (Samie et al, 2009, andAhmed et al, 2012) Shoot -anti-diabetic activity (Farag et al, 2015)…”

Section: Pharmacological Activitymentioning

confidence: 99%

Microscopic, molecular authentications, and flavonoid contents in selected bauhinia species and pharmacognostic specifications of bauhinia malabarica leaves

Thetsana

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There are more than 40 Bauhinia species throughout Thailand. Many pharmacologically active compounds have been reported, particularly flavonoids such as quercetin and its glycoside (quercitrin). This study aimed to characterize 20 Bauhinia species throughout Thailand using microscopic, molecular analysis as well as quercetin and quercitrin quantification. The pharmacognostic specification of B. malabarica leaf which has been used in Thai's remedies was established. Twenty Bauhinia species leaves were collected from 3 places throughout Thailand. The lamina of fresh mature leaves were microscopically evaluated for the stomatal number, stomatal index, palisade ratio, trichome number, trichome index, and epidermal cell area. The anatomy of midrib transverse sections were illustrated. ISSR-PCR was performed to classify these Bauhinia species. Preliminary quantification of quercetin and quercitrin in 20 Bauhinia species were done using RP-HPLC. Dried mature leaves were exhaustively extracted with 95% ethanol using Soxhlet apparatus. The extracts were injected to Inersil? ODS-3 C18 column and eluted by 0.5% phosphoric acid and methanol (1 : 1) at 35 ?C. Photo-diode array detector was set at 255 nm. Pharmacognostic specification of B. malabarica dried leaves collected from 15 places throughout Thailand was determined for the contents of loss on drying, water, total ash, acid insoluble ash, extractive matter as well as active compounds (quercetin and quercitrin). Chemical fingerprint of the ethanolic extracts was performed by TLC. All twenty Bauhinia species showed paracytic stomata type. B. aureifolia, B. bracteata, B. integrifolia, B, lakhonensis, B. purpurea, B. scandens, B. strychnifolia, B. variegata, and B. winitii were amphistomatic. Unicellular and multicellular nonglandular trichomes were found except B. integrifolia, B. pulla, B. scandens, B. sirindhorniae, B. strychnifolia, and B. winitii contained no trichomes. B. winitii showed two layers of palisade cells at upper epidermis. B. purpurea displayed the highest numbers of stomata (1120 1208 per mm2). B. saccocalyx had trichome number upto 200 per mm2 of lower epidermis. The anatomical characteristics of the midribs were illustrated. Their uniqueness could be used for species identification. Six ISSR primers produced 100% polymorphic DNA bands. A dendrogram generated by UPGMA could classify Bauhinia species in this study especially B. malabarica. Phytochemical analysis revealed quercetin and/or quercitrin contents in dried leaves of these 20 Bauhinia species. The highest contents of quercetin and quercitrin were found in B. malabarica. Pharmacognostic specification of B. malabarica dried leaves revealed the loss on drying, total ash, acid insoluble ash, and water contents should be not more than 8.00, 7.08, 1.79, and 8.28 g/100 g while ethanol and water soluble extractive matters should be not less than 13.78 and 16.47 g/100 g of dried leaves respectively. TLC-fingerprint was demonstrated. The contents of quercetin and quercitrin were 0.1796 ? 0.0678 and 0.3833 ? 0.2138 g/100 g of dried leaves, respectively.

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Section: Pharmacological Activitymentioning

confidence: 99%

Microscopic, molecular authentications, and flavonoid contents in selected bauhinia species and pharmacognostic specifications of bauhinia malabarica leaves

Thetsana

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Natural Products from Medicinal Plants against Phytopathogenic Fusarium Species: Current Research Endeavours, Challenges and Prospects

2021

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Many Fusarium species are pathogenic, causing crop diseases during crop production and spoilage of agricultural products in both commercial and smallholder farming. Fusarium attack often results into food contamination, yield loss and increases in food insecurity and food prices. Synthetic fungicides have been used as a control strategy for the management of crop diseases caused by Fusarium pathogens. The negative effects associated with application of many synthetic pesticides has necessitated the need to search for alternative control strategies that are affordable and environmentally safe. Research on medicinal plants as control agents for Fusarium pathogens has received attention since plants are readily available and they contain wide variety of secondary metabolites that are biodegradable. The activities of solvent extracts, essential oils and compounds from medicinal plants have been tested against Fusarium phytopathogenic species. A summary of recent information on antifungal activity of plants against Fusarium species is valuable for the development of biopesticides. This paper reviews the antifungal research conducted on medicinal plants against Fusarium pathogens, over a 10-year period, from January 2012 to May 2021. We also highlight the challenges and opportunities of using natural products from medicinal plants in crop protection. Several databases (Science Direct and Web of Science) were used to obtain information on botanical products used to control Fusarium diseases on crops. Keywords search used included natural products, antifungal, Fusarium, crops diseases, phytopathogenic, natural compounds and essential oil.

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Antifungal and antioxidant properties of ten medicinal plants collected from KwaDlangezwa area, KwaZulu-Natal Province

Cited by 2 publications

References 0 publications

Microscopic, molecular authentications, and flavonoid contents in selected bauhinia species and pharmacognostic specifications of bauhinia malabarica leaves

Microscopic, molecular authentications, and flavonoid contents in selected bauhinia species and pharmacognostic specifications of bauhinia malabarica leaves

Natural Products from Medicinal Plants against Phytopathogenic Fusarium Species: Current Research Endeavours, Challenges and Prospects

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