Antifungal activity of volatile organic compounds from Trichoderma virens

Inayati, Alfi; Sulistyowati, Liliek; Aini, Luqman Qurata; Yusnawan, Eriyanto

doi:10.1063/1.5115750

Cited by 15 publications

(11 citation statements)

References 61 publications

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“…However, some strains like Tv2 showed early lytic enzymes activity which allows them to degraded host cell walls and parasite them for their own growth. The researcher's previous study showed that volatile organic compounds produced by T. virens strains could inhibit the growth of R. solani up to 72% (Inayati, Sulistyowati, Aini, & Yusnawan, 2019) which supported the fact that other compounds of sesquiterpenes and fatty acids involved in the mycoparasitism of T. virens.…”

Section: Tv1mentioning

confidence: 78%

“…on dual culture assay (Yusnawan, Inayati, & Baliadi, 2019). The volatile organic compounds produced by indigenous T. virens also inhibited the growth of R. solani through its antifungal activity ( (Inayati, Sulistyowati, Aini, & Yusnawan, 2019). During the study, there was variation in inhibition capability among T. virens strains against a certain pathogen, for example, strains Tv6 showed high antagonist activity on dual culture assay, however not showed growth inhibition on R. solani growth on double plate assay indicating a specific action mode from T. virens strains on their mycoparasitism.…”

Section: Introductionmentioning

confidence: 99%

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Mycoparasitic Activity of Indigenous Trichoderma virens Strains Against Mungbean Soil Borne Pathogen Rhizoctonia solani: Hyperparasite and Hydrolytic Enzyme Production

et al. 2020

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Rhizoctonia solani is one of the harmful pathogens on mungbean, which is very challenging to be controlled. T. virens has been studied intensively and has great potency to control R. solani through mycoparasitism. Seven strains of T. virens isolated from various rhizospheres were tested for their mycoparasitic potential by observing their hyperparasitism and the production of hydrolytic enzymes. All strains showed the ability to suppress the growth of R. solani on dual culture assay as well as on culture filtrate test with the inhibition ability from 43.8 to 68.6% on the dual culture assay and from 22.2 to 71.1% on the culture filtrate assay. Inter-fungal interaction, which was observed by an electron microscope, showed hyperparasitic action of T. virens against R. solani involved the formation of the knob-like structure followed by the growth of Trichoderma hyphae inside host mycelia, coiling, lysed cell wall, and swollen of mycelial tips. Mycoparasitism of T. virens also correlated with the synthesis of hydrolytic enzymes, such as cellulase and chitinase, which influenced the overall hyperparasitic ability of T. virens against the pathogen. Based on in vitro assay, the Tv3 strain proposed as a promising strain to control R. solani due to its high growth inhibition and relatively high cellulase and chitinase productionse.

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Section: Tv1mentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Mycoparasitic Activity of Indigenous Trichoderma virens Strains Against Mungbean Soil Borne Pathogen Rhizoctonia solani: Hyperparasite and Hydrolytic Enzyme Production

et al. 2020

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“…sp. ‘ “ atroviride B” LU132 grown on plant synthetic medium (MS) [ 13 ] and other species of Trichoderma , including Trichoderma atroviride sensu stricto grown alone [ 28 , 49 , 50 , 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. “atroviride B”

Jong

Kandula

Rostás

et al. 2023

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Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactivity of VOCs from different Trichoderma species is well documented, information on intraspecific variation is limited. The fungistatic activity of VOCs emitted by 59 Trichoderma sp. “atroviride B” isolates against the pathogen Rhizoctonia solani was investigated. Eight isolates representing the two extremes of bioactivity against R. solani were also assessed against Alternaria radicina, Fusarium oxysporum f. sp. lycopersici and Sclerotinia sclerotiorum. VOCs profiles of these eight isolates were analyzed using gas chromatography–mass spectrometry (GC-MS) to identify a correlation between specific VOCs and bioactivity, and 11 VOCs were evaluated for bioactivity against the pathogens. Bioactivity against R. solani varied among the fifty-nine isolates, with five being strongly antagonistic. All eight selected isolates inhibited the growth of all four pathogens, with bioactivity being lowest against F. oxysporum f. sp. lycopersici. In total, 32 VOCs were detected, with individual isolates producing between 19 and 28 VOCs. There was a significant direct correlation between VOC number/quantity and bioactivity against R. solani. 6-pentyl-α-pyrone was the most abundant VOC produced, but 15 other VOCs were also correlated with bioactivity. All 11 VOCs tested inhibited R. solani growth, some by >50%. Some of the VOCs also inhibited the growth of the other pathogens by >50%. This study demonstrates significant intraspecific differences in VOC profiles and fungistatic activity supporting the existence of biological diversity within Trichoderma isolates from the same species, a factor in many cases ignored during the development of biological control agents.

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“…The methods that were used to prepare the Trichoderma cultures and to determine the VOCs were adopted from Inayati et al [30]. Initially, T. virens T.v3 and T. asperellum T.a8 were separately grown on PDA containing 0.50 M of NaCl for 4 days.…”

Section: Identification Of Volatile Organic Compounds From Salt-toler...mentioning

confidence: 99%

Changes in Volatile Organic Compounds from Salt-Tolerant Trichoderma and the Biochemical Response and Growth Performance in Saline-Stressed Groundnut

et al. 2021

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Soil salinity is one of the major obstacles that is limiting the growth and yield of groundnut. This study aims to investigate the effect of growth-promoting fungi, Trichoderma, on groundnut plants that were cultivated in saline conditions. Five different Trichoderma isolates were grown in four different NaCl concentrations. Selected Trichoderma were then applied to the groundnut seeds and their growth and development were monitored during the study. Growth inhibition, volatile organic compounds, chlorophylls, carotenoids, total phenolics and flavonoids, and minerals were assessed between the Trichoderma treatments. Increasing the salt concentration from 0.25–0.75 M decreased the growth of the Trichoderma isolates. The amounts and profiles of the volatile organic compounds from the T. asperellum isolate were significantly different to those in the T. virens isolate. In the vegetative growth stage, increased chlorophyll content was recorded in both the T. asperellum and T. virens-treated groundnut. The leaves that were obtained from the groundnut that was treated with T. virens T.v4 contained significantly higher indole-3-acetic acid (420 µg IAA/g) than the same plants’ roots (113.3 µg IAA/g). Compared to the control groundnut, the T. asperellum T.a8-treated groundnut showed increased phenolics (31%) and flavonoids (43%) and increased shoots and biomass weight at the generative growth stage. This study demonstrates that Trichoderma, with their plant growth promotion ability, could potentially be used to improve the growth of groundnut growing under salinity stress. Importantly, salt-tolerant Trichoderma could be regarded as a beneficial and sustainable way to improve the survival of salt-sensitive crops.

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Antifungal activity of volatile organic compounds from Trichoderma virens

Cited by 15 publications

References 61 publications

Mycoparasitic Activity of Indigenous Trichoderma virens Strains Against Mungbean Soil Borne Pathogen Rhizoctonia solani: Hyperparasite and Hydrolytic Enzyme Production

Mycoparasitic Activity of Indigenous Trichoderma virens Strains Against Mungbean Soil Borne Pathogen Rhizoctonia solani: Hyperparasite and Hydrolytic Enzyme Production

Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. “atroviride B”

Changes in Volatile Organic Compounds from Salt-Tolerant Trichoderma and the Biochemical Response and Growth Performance in Saline-Stressed Groundnut

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