Antifungal Activity of Volatile Organic Compounds from Bacillus velezensis CE 100 against Colletotrichum gloeosporioides

Choub, Vantha; Won, Sang-Jae; Ajuna, Henry B.; Moon, Jae-Hyun; Choi, Su-In; Lim, Hyo-In; Ahn, Young Sang

doi:10.3390/horticulturae8060557

Cited by 32 publications

(19 citation statements)

References 57 publications

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“…In the present study, inhibition of A. alternata and S. rolfsii growth by mVOCs emitted by both bacterial strains was observed under control conditions (0 mM NaCl). In agreement with our results, it has been shown that some mVOCs produce the inhibition of the vital activity of fungi in the soil, thereby negatively affecting the germination and growth of fungi [ 73 , 74 ]. Ossowicki et al [ 75 ] also showed this effect, when two fungal strains Rhizoctonia solani AG2.2IIIB and Fusarium culmorum PV were used against the Pseudomonas donghuensis strain P482, with the same being reported for the P. fluorescens ZX strain against Penicillium italicum [ 76 ].…”

Section: Discussionsupporting

confidence: 92%

Antifungal Activity and Alleviation of Salt Stress by Volatile Organic Compounds of Native Pseudomonas Obtained from Mentha piperita

Gil

Cappellari

Giordano

et al. 2023

Plants

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As salt stress has a negative impact on plant growth and crop yield, it is very important to identify and develop any available biotechnology which can improve the salt tolerance of plants. Inoculation with plant-growth-promoting rhizobacteria (PGPR) is a proven environmentally friendly biotechnological resource for increasing the salt stress tolerance of plants and has a potential in-field application. In addition, bacterial volatile organic compounds (mVOCs) are signal molecules that may have beneficial roles in the soil–plant–microbiome ecosystem. We investigated the effects of mVOCs emitted by Pseudomona putida SJ46 and SJ04 on Mentha piperita grown under different levels of NaCl stress by evaluating their growth-promoting potential and capacity to increase salt tolerance effects. Furthermore, we evaluated under control and salt stress conditions the biocontrol ability of VOCs emitted by both these strains to inhibit the growth of Alternaria alternata and Sclerotium rolfsii. The VOCs emitted by both strains under control conditions did not lead to an significant improvement in peppermint growth. However, under salt stress conditions (75 or 100 mM NaCl), an amelioration of its physiological status was observed, with this effect being greater at 100 mM NaCl. This led to an enhancement of the number of leaves and nodes and, increased the shoot fresh and root dry weight by approximately twice in relation to control stressed plants. Moreover, the VOCs released by the two bacteria grown in control or saline media showed a significant reduction in the mycelial growth of A. alternata. In contrast, S. rolfsii growth was reduced 40% by the mVOCs released only under control conditions, with no effects being observed under salt stress. We also explored the composition of the bacterial volatile profiles by means of a solid-phase microextraction/gas chromatography–mass spectrometry (SPME/GC–MS) analysis. From the headspace of SJ46, three VOCs were identified: n-octanol, decane and tetradecane. The emission of SJ04 had the same chromatographic profile, with the addition of two more compounds: 1-(N-phenyl carbamyl)-2-morpholino cyclohexene and tridecane. Only compounds that were not present in the headspace of the control groups were recorded. The salt stress conditions where the bacteria were grown did not qualitatively modify the mVOC emissions. Taken together, our results suggest that plant-associated rhizobacterial VOCs play a potentially important role in modulating plant salt tolerance and reducing fungal growth. Thus, biological resources represent novel tools for counteracting the deleterious effects of salt stress and have the potential to be exploited in sustainable agriculture. Nevertheless, future studies are necessary to investigate technological improvements for bacterial VOC application under greenhouse and open field conditions.

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Section: Discussionsupporting

confidence: 92%

Antifungal Activity and Alleviation of Salt Stress by Volatile Organic Compounds of Native Pseudomonas Obtained from Mentha piperita

Gil

Cappellari

Giordano

et al. 2023

Plants

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“…Twisted hyphae were also observed in Colletotrichum acutatum, Colletotrichum coccodes, Colletotrichum dematium, and Colletotrichum gloeosporioides after treatment with the VOC mixture produced by B. velezensis CE 100 [ 74 ]. VOCs of B. velezensis JRX-YG39 have caused hyphae abnormalities such as coiling and discoloration in Colletotrichum gloeosporioides (walnut and jujube anthracnose), while the pure compounds 5-nonylamine and 3-methylbutanoic acid have caused similar morphological changes followed by cell wall lysis and fracturing [ 75 ].…”

Section: Mechanisms Of Action Of Antifungal Bacillus ...mentioning

confidence: 99%

“…A significant decrease in spore density of Fusarium verticillioides and Fusarium graminearum was observed after treatment with B. mojavensis I4 VOCs [ 76 ]. The different concentrations of 1%, 3%, 5%, 7%, and 10% of the VOCs produced by B. velezensis CE 100 reduced the spore germination of Colletotrichum gloeosporioides (walnut and jujube antrachnose) by 15.4%, 18.9%, 25.4%, 30.0%, and 36.4%, respectively, with remarkably reduced germ tube elongation [ 75 ]. Incompact and irregular structures of spores of Verticillium dahliae , causing cotton Verticilium wilt, together with visible holes on the spores’ surface, appeared after treatment using styrene produced by Bacillus sp.…”

Section: Mechanisms Of Action Of Antifungal Bacillus ...mentioning

confidence: 99%

Bacillus VOCs in the Context of Biological Control

2023

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A contemporary agricultural production system relying on heavy usage of agrochemicals represents a questionable outlook for sustainable food supply in the future. The visible negative environmental impacts and unforeseen consequences to human and animal health have been requiring a shift towards the novel eco-friendly alternatives for chemical pesticides for a while now. Microbial-based biocontrol agents have shown a promising potential for plant disease management. The bacteria of the genus Bacillus have been among the most exploited microbial active components due to several highly efficient mechanisms of action against plant pathogens, as well as a palette of additional plant-beneficial mechanisms, together with their suitable properties for microbial biopesticide formulations. Among other bioactive metabolites, volatile organic compounds (VOCs) have been investigated for their biocontrol applications, exhibiting the main advantage of long-distance effect without the necessity for direct contact with plants or pathogens. The aim of this study is to give an overview of the state-of-the-art in the field of Bacillus-based VOCs, especially in terms of their antibacterial, antifungal, and nematicidal action as the main segments determining their potential for biocontrol applications in sustainable agriculture.

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“…While they are not considered to be phytohormones per se, the volatile organic compounds (VOCs) that are synthesized by a wide range of soil bacteria have recently been shown to play a significant role in plant growth promotion [ 6 , 31 , 32 , 33 , 34 ]. The growth promotion effect of VOCs is largely attributed to the modulation of the synthesis and/or metabolism of known phytohormones, synthesized by either PGPB or by plants [ 35 , 36 ]. The bacterial synthesis of VOCs has been shown to increase plant photosynthesis, and the content of gibberellin, auxin, and cytokinin, depending upon the plant and the particular organic compounds involved.…”

Section: Volatile Organic Compoundsmentioning

confidence: 99%

Recent Advances in the Bacterial Phytohormone Modulation of Plant Growth

Orozco-Mosqueda

Santoyo

Glick

2023

Plants

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Phytohormones are regulators of plant growth and development, which under different types of stress can play a fundamental role in a plant’s adaptation and survival. Some of these phytohormones such as cytokinin, gibberellin, salicylic acid, auxin, and ethylene are also produced by plant growth-promoting bacteria (PGPB). In addition, numerous volatile organic compounds are released by PGPB and, like bacterial phytohormones, modulate plant physiology and genetics. In the present work we review the basic functions of these bacterial phytohormones during their interaction with different plant species. Moreover, we discuss the most recent advances of the beneficial effects on plant growth of the phytohormones produced by PGPB. Finally, we review some aspects of the cross-link between phytohormone production and other plant growth promotion (PGP) mechanisms. This work highlights the most recent advances in the essential functions performed by bacterial phytohormones and their potential application in agricultural production.

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Antifungal Activity of Volatile Organic Compounds from Bacillus velezensis CE 100 against Colletotrichum gloeosporioides

Cited by 32 publications

References 57 publications

Antifungal Activity and Alleviation of Salt Stress by Volatile Organic Compounds of Native Pseudomonas Obtained from Mentha piperita

Antifungal Activity and Alleviation of Salt Stress by Volatile Organic Compounds of Native Pseudomonas Obtained from Mentha piperita

Bacillus VOCs in the Context of Biological Control

Recent Advances in the Bacterial Phytohormone Modulation of Plant Growth

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