Antifungal activity of volatile compounds produced by endophytic Bacillus subtilis DZSY21 against Curvularia lunata

Xie, Shanshan; Liu, Juan; Gu, Shuangyue; Chen, Xiaojie; Jiang, Haiyang; Ding, Tao

doi:10.1186/s13213-020-01553-0

Cited by 41 publications

(32 citation statements)

References 30 publications

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“…Using a modified dual culture (cross-streak) method, the antagonistic activity of endophytic bacterial strains against virulent phytopathogens ( Alternaria solani , Rhizoctonia solani , Fusarium oxysporum and Ustilaginoidea virens ) was examined in vitro [ 24 ]. On potato dextrose agar (PDA) plates, a 5-mm disc of freshly cultured fungal cultures was implanted with a bacterial culture introduced on one side of the fungal plug.…”

Section: Methodsmentioning

confidence: 99%

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

Shahid

Zeyad

Syed

et al. 2022

IJERPH

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In efforts to improve plant productivity and enhance defense mechanisms against biotic and abiotic stresses, endophytic bacteria have been used as an alternative to chemical fertilizers and pesticides. In the current study, 25 endophytic microbes recovered from plant organs of Triticum aestivum L. (wheat) were assessed for biotic (phyto-fungal pathogens) and abiotic (salinity, drought, and heavy metal) stress tolerance. Among the recovered isolates, BPR-9 tolerated maximum salinity (18% NaCl), drought (15% PEG-6000), and heavy metals (µg mL−1): Cd (1200), Cr (1000), Cu (1000), Pb (800), and Hg (30). Based on phenotypic and biochemical characteristics, as well as 16S rDNA gene sequencing, endophytic isolate BPR-9 was recognized as Priestia aryabhattai (accession no. OM743254.1). This isolate was revealed as a powerful multi-stress-tolerant crop growth promoter after extensive in-vitro testing for plant growth-promoting attributes, nutrient (phosphate, P; potassium, K; and zinc, Zn) solubilization efficiency, extracellular enzyme (protease, cellulase, amylase, lipase, and pectinase) synthesis, and potential for antagonistic activity against important fungal pathogens viz. Alternaria solani, Rhizoctonia solani, Fusarium oxysporum, and Ustilaginoidea virens. At elevated salt levels, increases were noted in indole-3-acetic acid; siderophores; P, K, and Zn-solubilization; ACC deaminase; and ammonia synthesized by Priestia aryabhattai. Additionally, under in-vitro plant bioassays, wheat seedlings inoculated with P. aryabhattai experienced superior growth compared to non-inoculated seedlings in high salinity (0–15% NaCl) environment. Under NaCl stress, germination rate, plant length, vigor indices, and leaf pigments of wheat seedlings significantly increased following P. aryabhattai inoculation. Furthermore, at 2%-NaCl, B. aryabhattai greatly and significantly (p ≤ 0.05) decreased relative leaf water content, membrane damage, and electrolyte leakage compared with the non-inoculated control. Catalase, superoxide dismutase, and peroxidase activity increased by 29, 32, and 21%, respectively, in wheat seedlings exposed to 2% NaCl and inoculated with the bacteria. The present findings demonstrate that endophytic P. aryabhattai strains might be used in the future as a multi-stress reducer and crop growth promoter in agronomically important crops including cereals.

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Section: Methodsmentioning

confidence: 99%

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

Shahid

Zeyad

Syed

et al. 2022

IJERPH

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“…Endophytic PGPR Bacillus subtilis strain DZSY21 was reported to inhibit the mycelia growth and conidial sporulation of fungal pathogen Curvularia lunata by producing VOCs like 2-methylbutyric acid, 2-heptanone, and isopentyl acetate. Application of VOCs in maize leaves was observed to reduce disease indexes from 60.52 to 26.64%, further isopentyl acetate showed enhancement in accumulation of intracellular reactive oxygen species (ROS) in conidia [ 170 ]. VOCs (2,5-dimethylpyrazine and benzothiazole) produced by endophytic PGPR B. velezensis ZSY-1 was found to have strong antifungal activity toward Alternaria solani and Botrytis cinerea [ 171 ].…”

Section: Rhizosphere and Root Endophytesmentioning

confidence: 99%

The Endophytic Microbiome as a Hotspot of Synergistic Interactions, with Prospects of Plant Growth Promotion

Vandana

Rajkumari

Singha

et al. 2021

Biology

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The plant root is the primary site of interaction between plants and associated microorganisms and constitutes the main components of plant microbiomes that impact crop production. The endophytic bacteria in the root zone have an important role in plant growth promotion. Diverse microbial communities inhabit plant root tissues, and they directly or indirectly promote plant growth by inhibiting the growth of plant pathogens, producing various secondary metabolites. Mechanisms of plant growth promotion and response of root endophytic microorganisms for their survival and colonization in the host plants are the result of complex plant-microbe interactions. Endophytic microorganisms also assist the host to sustain different biotic and abiotic stresses. Better insights are emerging for the endophyte, such as host plant interactions due to advancements in ‘omic’ technologies, which facilitate the exploration of genes that are responsible for plant tissue colonization. Consequently, this is informative to envisage putative functions and metabolic processes crucial for endophytic adaptations. Detection of cell signaling molecules between host plants and identification of compounds synthesized by root endophytes are effective means for their utilization in the agriculture sector as biofertilizers. In addition, it is interesting that the endophytic microorganism colonization impacts the relative abundance of indigenous microbial communities and suppresses the deleterious microorganisms in plant tissues. Natural products released by endophytes act as biocontrol agents and inhibit pathogen growth. The symbiosis of endophytic bacteria and arbuscular mycorrhizal fungi (AMF) affects plant symbiotic signaling pathways and root colonization patterns and phytohormone synthesis. In this review, the potential of the root endophytic community, colonization, and role in the improvement of plant growth has been explained in the light of intricate plant-microbe interactions.

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“…Also, the derivative of 1, 3-butylene glycol exhibited potent antibacterial and antifungal activities [39]. Similarly, the compound isopentyl acetate from endophytic Bacillus subtilis inhibits the growth and sporulation of Curvularia lunata [40]. Cymene isomers are reported to possess insecticidal and repellent properties [41].…”

Section: Biochemical Analysismentioning

confidence: 99%

Antibacterial Activity of Ethyl Acetate Extract of Endophytic Fungus (Paraconiothyrium Brasiliense) Through Targeting Pathogenesis Related Dihydropteroate Synthase (DHPS)

Sathiyaseelan

Saravanakumar

Anand

et al. 2021

Preprint

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Endophytic fungi are innumerable sources of bioactive metabolites with various biomedical applications. Hence, this study was aimed to isolate and identify the endophytic fungus Paraconiothyrium brasiliense (PB) from the fruit of Capsicum annuum and evaluated their biochemical, antimicrobial, antioxidant, cell viability, and cytotoxicity behavior. Results showed that ethyl acetate extract (EAE) of PE contains flavonoid (31.53 ± 0.9 mg of QE/g of extract) and phenolic (2.59 ± 0.06 mg of GAE/g of extract). The PB-EAE showed significant antioxidant activity in terms of free radicals such as DPPH, ABTS, and Fe3+ scavenging. The antioxidant potential of PB-EAE reflects in normal cell viability and anticancer activity against prostate cancer (PC3) cells. The bacterial inhibitory activity of PB-EAE was equal to commercial antibiotic tetracycline hydrochloride (TCH). Further, GC-MS analysis confirmed the presence of volatile molecules such as o-cymene (CH3C6H4CH(CH3)2), dipentene (C10H16), γ-terpinene (C10H16), 4-carvomenthenol (C10H18O), heneicosane (CH3(CH2)19CH3), 2,5-piperazinedione (C4H6N2O2). Also, molecular docking analysis demonstrated the significant interactions between the compounds of PB-EAE and Dihydropteroate Synthase (Protein ID:5JQ9; DHPS). This study revealed that endophytic P. brasiliense from Capsicum annuum could be a novel source for the isolation of antimicrobials.

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Antifungal activity of volatile compounds produced by endophytic Bacillus subtilis DZSY21 against Curvularia lunata

Cited by 41 publications

References 30 publications

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

The Endophytic Microbiome as a Hotspot of Synergistic Interactions, with Prospects of Plant Growth Promotion

Antibacterial Activity of Ethyl Acetate Extract of Endophytic Fungus (Paraconiothyrium Brasiliense) Through Targeting Pathogenesis Related Dihydropteroate Synthase (DHPS)

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