Antifungal activity of the ribosome‐inactivating protein <scp>BE</scp>27 from sugar beet (<i><scp>B</scp>eta vulgaris</i> <scp>L</scp>.) against the green mould <i><scp>P</scp>enicillium digitatum</i>

Citores, Lucı́a; Iglesias, Rosario; Ferreras, José M.

doi:10.1111/mpp.12278

Cited by 30 publications

(39 citation statements)

References 60 publications

(145 reference statements)

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“…The antiviral activity of type 1 RIPs confirms their defensive role ([38,181], reviewed in [41]). In addition, these plant enzymes exhibit antibacterial [182,183], antifungal [184,185,186], anti-protozoan [187] and insecticidal [188] properties (reviewed in [189]).…”

Section: Biological Roles and Future Perspectivesmentioning

confidence: 99%

Ribosome-Inactivating Proteins from Plants: A Historical Overview

et al. 2016

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Abstract:This review provides a historical overview of the research on plant ribosome-inactivating proteins (RIPs), starting from the first studies at the end of eighteenth century involving the purification of abrin and ricin, as well as the immunological experiments of Paul Erlich. Interest in these plant toxins was revived in 1970 by the observation of their anticancer activity, which has given rise to a large amount of research contributing to the development of various scientific fields. Biochemistry analyses succeeded in identifying the enzymatic activity of RIPs and allowed for a better understanding of the ribosomal machinery. Studies on RIP/cell interactions were able to detail the endocytosis and intracellular routing of ricin, thus increasing our knowledge of how cells handle exogenous proteins. The identification of new RIPs and the finding that most RIPs are single-chain polypeptides, together with their genetic sequencing, has aided in the development of new phylogenetic theories. Overall, the biological properties of these proteins, including their abortifacient, anticancer, antiviral and neurotoxic activities, suggest that RIPs could be utilized in agriculture and in many biomedical fields, including clinical drug development.Keywords: anticancer drugs; antiviral proteins; immunotoxins; plant lectins; plant toxins; ribosome-inactivating proteins; ricin; science history The First StudiesA series of plants used from ancient times for medicinal purposes in various parts of the world (reviewed in [1]) produce proteins that are today referred to as ribosome-inactivating proteins (RIPs) (reviewed in [2]). Some of these RIP-expressing medicinal plants are very toxic, and their toxicity has been known since antiquity (reviewed in [3]).The history of RIPs began when the interest of the scientific community turned to the toxicity of plant poisons, in particular those from the seeds of Ricinus communis L. (castor bean) and Abrus precatorius L. (jequirity bean). In both cases, the toxins were identified at the end of nineteenth century to be proteins [4,5]. They were partially purified at the University of Dorpat (now Tartu in Estonia) and named ricin [6] and abrin [7], respectively. These studies, performed for a doctoral thesis, identified ricin and abrin as haemagglutinins, and their toxicity was erroneously attributed to their ability to agglutinate red blood cells.The next stage of RIP history involved the pioneering immunological research of Paul Ehrlich at the Institute of Infectious Diseases in Berlin. He observed protective effects from the toxicity of either abrin or ricin in mice by feeding them low amounts of the toxins. The immunized animals still conserved sensitivity to the other toxin, demonstrating the specificity of the immunity. He found that the serum of the immunized animals contained proteins that were able to precipitate the specific immunizing toxin but not the other toxin. He also found that the anti-toxin immunity could be transferred from the mother to the offspring both through bl...

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Section: Biological Roles and Future Perspectivesmentioning

confidence: 99%

Ribosome-Inactivating Proteins from Plants: A Historical Overview

et al. 2016

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“…The ultrastructural changes, such as cells with irregular budding sites, other aspect in the cytoplasm, the loss of integrity and rigidity of cell walls suggested that the antifungal mechanism of α-MMC was a complex process ( Wang et al, 2012 ). Recently, the antifungal activity of the RIP BE27 isolated from sugar beet was investigated against the green mold Penicillium digitatum ( Citores et al, 2016 ). They found that RIP BE27 can inhibit the growth of the fungus Penicillium digitatum because that it can enter the fungal cytoplasm and kill fungal cells ( Citores et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the antifungal activity of the RIP BE27 isolated from sugar beet was investigated against the green mold Penicillium digitatum ( Citores et al, 2016 ). They found that RIP BE27 can inhibit the growth of the fungus Penicillium digitatum because that it can enter the fungal cytoplasm and kill fungal cells ( Citores et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

et al. 2018

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Ribosome-inactivating proteins (RIPs) are toxic N-glycosidases that depurinate eukaryotic and prokaryotic rRNAs, thereby arresting protein synthesis during translation. RIPs are widely found in various plant species and within different tissues. It is demonstrated in vitro and in transgenic plants that RIPs have been connected to defense by antifungal, antibacterial, antiviral, and insecticidal activities. However, the mechanism of these effects is still not completely clear. There are a number of reviews of RIPs. However, there are no reviews on the biological functions of RIPs in defense against pathogens and insect pests. Therefore, in this report, we focused on the effect of RIPs from plants in defense against pathogens and insect pest attacks. First, we summarize the three different types of RIPs based on their physical properties. RIPs are generally distributed in plants. Then, we discuss the distribution of RIPs that are found in various plant species and in fungi, bacteria, algae, and animals. Various RIPs have shown unique bioactive properties including antibacterial, antifungal, antiviral, and insecticidal activity. Finally, we divided the discussion into the biological roles of RIPs in defense against bacteria, fungi, viruses, and insects. This review is focused on the role of plant RIPs in defense against bacteria, fungi, viruses, and insect attacks. The role of plant RIPs in defense against pathogens and insects is being comprehended currently. Future study utilizing transgenic technology approaches to study the mechanisms of RIPs will undoubtedly generate a better comprehending of the role of plant RIPs in defense against pathogens and insects. Discovering additional crosstalk mechanisms between RIPs and phytohormones or reactive oxygen species (ROS) against pathogen and insect infections will be a significant subject in the field of biotic stress study. These studies are helpful in revealing significance of genetic control that can be beneficial to engineer crops tolerance to biotic stress.

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“…Nonfungal (i.e., bacterial, plant, animal, and/or human) counterparts (Al-Mahrous et al 2011;Citores et al 2016;Iglesias et al 2016;Jiratchariyakul et al 2001;Ogawa 2016;Ouyang et al 2006;Sartim and Sampaio 2015;Singh et al 2016) of the various fungal proteins and peptides very often are endowed with biological activities qualitatively analogous to the fungal proteins and peptides, although the structural homology may not be extensive. However, some differences in biological activity may exist.…”

Section: Resultsmentioning

confidence: 99%

Fungal proteinaceous compounds with multiple biological activities

Cheung

Wong

et al. 2016

Appl Microbiol Biotechnol

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Fungi comprise organisms like molds, yeasts and mushrooms. They have been used as food or medicine for a long time. A large number of fungal proteins or peptides with diverse biological activities are considered as antibacterial, antifungal, antiviral and anticancer agents. They encompass proteases, ribosome inactivating proteins, defensins, hemolysins, lectins, laccases, ribonucleases, immunomodulatory proteins, and polysaccharopeptides. The target of the present review is to update the status of the various bioactivities of these fungal proteins and peptides and discuss their therapeutic potential.

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Antifungal activity of the ribosome‐inactivating protein BE27 from sugar beet (Beta vulgaris L.) against the green mould Penicillium digitatum

Cited by 30 publications

References 60 publications

Ribosome-Inactivating Proteins from Plants: A Historical Overview

Ribosome-Inactivating Proteins from Plants: A Historical Overview

The Plant Ribosome-Inactivating Proteins Play Important Roles in Defense against Pathogens and Insect Pest Attacks

Fungal proteinaceous compounds with multiple biological activities

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