Antifungal activity of sterols and dipsacus saponins isolated from Dipsacus asper roots against phytopathogenic fungi

Choi, Nam Hee; Jang, Ja Yeong; Choi, Gyung Ja; Choi, Yong Ho; Jang, Kyoung Soo; Nguyen, Van Thu; Min, Byung‐Sun; Dang, Quang Le; Kim, Jin-Cheol

doi:10.1016/j.pestbp.2016.12.006

Cited by 40 publications

(30 citation statements)

References 31 publications

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“…Our findings show that peptide extracts against P. infestans, as determined by optical microscopy, had a high level of inhibition. There are some mentions confirming the application of aquatic and organic plant extracts to suppress P. infestans and other phytopathogenic oomycetes; these are potent agents for biocontrol [ 38 , 39 , 40 , 41 ]. Generally, the target activity is associated with secondary metabolites of a different chemical nature and not with polypeptides.…”

Section: Discussionmentioning

confidence: 99%

Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease

Рогожин

Vasilchenko

Barashkova

et al. 2020

Plants

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We report the inhibitory effect of peptide extracts obtained from seven medicinal plants against a causative agent of late blight disease Phytophthora infestans. We find that all the extracts possess inhibitory activity toward the zoospores output, zoosporangium germination, and the development of P. infestans on potato disc tubers at different quantitative levels. Based on the biological effects detected, an extract of common horsetail (Equisetum arvense) biomass is recognized as the most effective and is selected for further structural analysis. We perform a combination of amino acid analysis and MALDI-TOF mass spectrometry, which reveal the presence of Asn/Asp- and Gln/Glu-rich short peptides with molecular masses in the range of 500–900 Da and not exceeding 1500 Da as the maximum. Analytical anion-exchange HPLC is successfully applied for separation of the peptide extract from common horsetail (E. arvense). We collect nine dominant components that are combined in two groups with differences in retention times. The N-terminal amino acid sequence of the prevalent compounds after analytical ion-exchange HPLC allows us to identify them as peptide fragments of functionally active proteins associated with photosynthesis, aquatic transport, and chitin binding. The anti-oomycete effects may be associated with the conversion of ribulose-1,5-bisphosphate carboxylase/oxygenase to produce a number of biologically active anionic peptides with possible regulatory functions. These data inform our knowledge regarding biologically active peptide fragments; they are the components of programmed or induced proteolysis of plant proteins and can realize secondary antimicrobial functions.

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Section: Discussionmentioning

confidence: 99%

Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease

Рогожин

Vasilchenko

Barashkova

et al. 2020

Plants

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“…hordei, B. cinerea , C. coccodes , F. oxysporum , M. oryzae , P. infestans , and P. triticina were used for the in vitro or in vivo antifungal activity assay. Fungal sporulation and maintenance were performed as previously described 8 , 11 .…”

Section: Methodsmentioning

confidence: 99%

“…After treatment with the plant extracts or TPGs, the plants were inoculated with a fungal pathogen and incubated as previously described 42 , 43 . The experiment was conducted twice with three replicates for each treatment, and the disease control efficacy was calculated with the following equation: control efficacy (%) = 100 × [1 − B/A], where A is the mean lesion area (%) on the leaves or sheaths of the control plants, and B is the mean lesion area (%) on the leaves or sheaths of the treated plants 11 , 42 , 43 .…”

Section: Methodsmentioning

confidence: 99%

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Identification of novel compounds, oleanane- and ursane-type triterpene glycosides, from Trevesia palmata: their biocontrol activity against phytopathogenic fungi

Kim

Han

Ngo

et al. 2018

Sci Rep

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Plants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. As part of our search for new antimicrobial agents from natural sources, we found that a crude methanol extract of Trevesia palmata exhibited a promising antifungal activity against phytopathogenic fungi, such as Magnaporthe oryzae and Botrytis cinerea. Furthermore, based on activity-guided fractionation, we isolated five antifungal compounds from the methanol extract of T. palmata: two new triterpene glycosides (TPGs), TPG1 (hederagenin-3-O-β-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranoside) and TPG5 (3-O-α-L-rhamnopyranosyl asiatic acid), along with three known TPGs (TPG2 [macranthoside A], TPG3 [α-hederin], and TPG4 [ilekudinoside D]). The chemical structures of the TPGs were determined by spectroscopic analyses and by comparison with literature data. An in vitro antifungal bioassay revealed that except for TPG4 (ilekudinoside D; IC50 >256 μg/ml), the other TPGs exhibited strong antifungal activities against the rice blast pathogen M. oryzae with IC50 values ranging from 2–5 μg/ml. In particular, when the plants were treated with compound TPG1 (500 μg/ml), disease control values against rice blast, tomato grey mold, tomato late blight, and wheat leaf rust were 84, 82, 88, and 70%, respectively, compared to the non-treatment control. Considering the in vitro and in vivo antifungal activities of the TPGs and the T. palmata methanol extracts, our results suggest that T. palmata can be a useful source to develop new natural fungicides.

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“…Notably β‐sitosterol was more effective against tomato gray mold than other pathogens with a 50% reduction in the development of the disease on tomato seedlings. The extract contained sterols and other compounds, and the anti‐fungal effect of the mixture was synergistic . β‐Sitosterol has also exhibited anti‐fungal activity against germination of spores of Fusarium moniliforme , Penicillium sclerotigenum and Cladosporium cladosporioides to various extents (≤ 57%) at a concentration of 50 mg L −1 .…”

Section: Introductionmentioning

confidence: 99%

The anti‐fungal β‐sitosterol targets the yeast oxysterol‐binding protein Osh4

Moosavi

Liu

Wang

et al. 2019

Pest Management Science

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BACKGROUND: -Sitosterol is a plant metabolite with a broad range of anti-fungal activity, however, this compound is not toxic against a few fungal species. The target of -sitosterol and the nature of its selective toxicity are not yet clear. Using a yeast model system and taking advantage of molecular biology and computational approaches, we identify the target and explain why -sitosterol is not toxic against some fungal pathogens. RESULTS: -Sitosterol (200 g mL −1 ) is toxic against yeast cells expressing only Osh4 (an oxysterol-binding protein) andharbouring a upc2-1 mutation (which enables sterol uptake), but not against yeast strains expressing all seven Osh proteins and harbouring a upc2-1 mutation. Furthermore, -sitosterol is not toxic against yeast strains without the upc2-1 mutation irrespective of the number of Osh proteins being expressed. The deletion of COQ1 (a gene known to be highly induced upon deletion of OSH4) enhances the toxicity of -sitosterol in yeast cells expressing only Osh4 and harbouring the upc2-1 mutation. Molecular modelling suggests that -sitosterol binds to Osh4 and the binding mode is similar to the binding of cholesterol to Osh4. CONCLUSION:Our results indicate that the concentrations of -sitosterol, and Osh4, as well as its homologues within cells, are most likely the main determinants of -sitosterol toxicity. Furthermore, some fungal species do not take up sterols, e.g. Saccharomyces cerevisiae, under aerobic conditions. Therefore, sterol uptake may also contribute to the -sitosterol anti-fungal effect. These findings enable predicting the toxicity of -sitosterol against plant fungal pathogens.

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Antifungal activity of sterols and dipsacus saponins isolated from Dipsacus asper roots against phytopathogenic fungi

Cited by 40 publications

References 31 publications

Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease

Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease

Identification of novel compounds, oleanane- and ursane-type triterpene glycosides, from Trevesia palmata: their biocontrol activity against phytopathogenic fungi

The anti‐fungal β‐sitosterol targets the yeast oxysterol‐binding protein Osh4

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