Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella fijiensis, causal agent of black Sigatoka in banana (Musa spp.)

The aim of the present study is to evaluate plant growth promoting and biocontrol efficacy of a Serratia marcescens strain ETR17 isolated from tea rhizosphere for the effective management of root rot disease in tea. Isolated bacterial culture ETR17 showed significant level of in vitro antagonism against nine different foliar and root pathogens of tea. The phenotypic and molecular characterization of ETR17 revealed the identity of the bacterium as Serratia marcescens. The bacterium was found to produce several hydrolytic enzymes like chitinase, protease, lipase, cellulase and plant growth promoting metabolites like IAA and siderophore. Scanning electron microscopic studies on the interaction zone between pathogen and antagonistic bacterial isolate revealed severe deformities in the fungal mycelia. Spectral analyses (LC-ESI-MS, UV-VIS spectrophotometry and HPLC) and TLC indicated the presence of the antibiotics pyrrolnitrin and prodigiosin in the extracellular bacterial culture extracts. Biofilm formation by ETR17 on polystyrene surface was also observed. In vivo application of talc-based formulations prepared with the isolate ETR17 in tea plantlets under green house conditions revealed effective reduction of root-rot disease as well as plant growth promotion to a considerable extent. Viability studies with the ETR17 talc formulation showed the survivability of the isolate up to six months at room temperature. The sustenance of ETR17 (concentration of 8-9x108 cfu g-1) in the soil after the application of talc formulation was recorded by ELISA. Safety studies revealed that ETR17 did not produce hemolysin as observed in pathogenic Serratia strains. The biocontrol strain reported in this study can be used for field application in order to minimize the use of chemical fungicides for disease control in tea gardens.

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“…Prodigiosin purified from S . marcescens controlled Black Sigatoka in banana by a synergistic action with purified chitinases [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot disease in tea

2018

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“…A synergistic effect of the S. marcescens B2 produced PG and chitinolytic enzymes was also observed against the tested fungal pathogens [ 85 , 86 , 87 ]. Gutiérrez-Román et al [ 88 ] also reported that the combination of S. marcescens CFFSUR-B2 produced PG and chitinolytic enzymes showed synergistic inhibitory effect on the germination and germ tube growth of Mycosphaerella fijiensis ascospores. Based on the results of toxic effects similar to that of benzimidazole on ascospore germination, the authors suggested that the combination of PG and chitinolytic enzymes may have potential use in the biocontrol of black Sigatoka disease caused by Mycosphaerella fijiensis [ 88 ].…”

Section: Bioactivity and Application Of Pgmentioning

confidence: 99%

“…Consequently, the uses of bacterial pigments have expanded much more than synthetic pigments [ 80 ]. Prodiginines (PGs), a family of red microbial pigments, have received much attention because of their considerable bioactivities [ 59 , 60 , 61 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 …”

Section: Introductionmentioning

confidence: 99%

“…For example, a review of the characteristics and potential therapeutic anticancer-drug applications of PG from Serratia was introduced by Darshan and Manonmani [ 180 ], while the structure, chemical synthesis, and biosynthesis of PGs as natural products were summarized by Hu et al [ 157 ]. Additionally, synergistic inhibitory effects of chitinolytic enzymes [ 36 , 37 , 39 , 86 , 87 , 88 , 89 , 90 ], proteolytic enzymes [ 36 , 61 , 89 , 192 , 193 , 194 , 195 ], and S. marcescens PG were estimated for biocontrol in plant cultivation [ 85 , 86 , 87 , 88 , 89 , 90 ]. Biosurfactant produced by S. marcescens was also evaluated [ 167 , 196 , 197 , 198 ].…”

Section: Introductionmentioning

confidence: 99%

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Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

et al. 2020

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The technology of microbial conversion provides a potential way to exploit compounds of biotechnological potential. The red pigment prodigiosin (PG) and other PG-like pigments from bacteria, majorly from Serratia marcescens, have been reported as bioactive secondary metabolites that can be used in the broad fields of agriculture, fine chemicals, and pharmacy. Increasing PG productivity by investigating the culture conditions especially the inexpensive carbon and nitrogen (C/N) sources has become an important factor for large-scale production. Investigations into the bioactivities and applications of PG and its related compounds have also been given increased attention. To save production cost, chitin and protein-containing fishery byproducts have recently been investigated as the sole C/N source for the production of PG and chitinolytic/proteolytic enzymes. This strategy provides an environmentally-friendly selection using inexpensive C/N sources to produce a high yield of PG together with chitinolytic and proteolytic enzymes by S. marcescens. The review article will provide effective references for production, bioactivity, and application of S. marcescens PG in various fields such as biocontrol agents and potential pharmaceutical drugs.

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“…Also examined was the expression of genes that are involved in canonical S. marcescens antifungal pathways. Known antifungal pathways include the production of broadly antifungal enzymes such as extracellular chitinases and glucanases, as well as the production of secondary metabolites such as prodigiosin (Duzhak et al, 2012 ; Gutiérrez-Román et al, 2015 ; Tan et al, 2015 ). Elucidations from this data will allow for more in-depth studies to occur on the mechanisms by which S. marcescens inhibits Bd growth.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Batrachochytrium dendrobatidis Inhibiting Bacteria from Amphibian Populations in Costa Rica

et al. 2017

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Global amphibian declines and extinction events are occurring at an unprecedented rate. While several factors are responsible for declines and extinction, the fungal pathogen Batrachochytrium dendrobatidis (Bd) has been cited as a major constituent in these events. While the effects of this chytrid fungus have been shown to cause broad scale population declines and extinctions, certain individuals and relict populations have shown resistance. This resistance has been attributed in part to the cutaneous bacterial microbiome. Here, we present the first study characterizing anti-Bd bacterial isolates from amphibian populations in Costa Rica, including the characterization of two strains of Serratia marcescens presenting strong anti-Bd activity. Transcriptome sequencing was utilized for delineation of shifts in gene expression of the two previously uncharacterized strains of S. marcescens grown in three different treatments comprising Bd, heat-killed Bd, and a no Bd control. These results revealed up- and down-regulation of key genes associated with different metabolic and regulatory pathways. This information will be valuable in continued efforts to develop a bacterial-based approach for amphibian protection as well as providing direction for continued mechanistic inquiries of the bacterial anti-Bd response.

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Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella fijiensis, causal agent of black Sigatoka in banana (Musa spp.)

Cited by 33 publications

References 23 publications

Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot disease in tea

Evaluation of the biocontrol efficacy of a Serratia marcescens strain indigenous to tea rhizosphere for the management of root rot disease in tea

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

Characterization of Batrachochytrium dendrobatidis Inhibiting Bacteria from Amphibian Populations in Costa Rica

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