Antifungal activity of Saccharomyces cerevisiae peroxisomal 3-ketoacyl-CoA thiolase

Lee, Jung Ro; Kim, Sun Young; Chae, Ho Byoung; Jung, Ji Hyun; Lee, Sang Yeol

doi:10.5483/bmbrep.2009.42.5.281

Cited by 7 publications

(6 citation statements)

References 22 publications

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“…7). In order to verify peroxisomal localization, both KatG and the peroxisomal marker protein 3-ketoacyl-CoA-thiolase (EC 2.3.1.16) (Goh et al 2011; Lee et al 2009) were probed by polyclonal anti-KatG (Fig. 7b) and anti-Fox3 antibodies (Fig.…”

Section: Resultsmentioning

confidence: 99%

Intracellular targeting of ascomycetous catalase-peroxidases (KatG1s)

et al. 2013

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Bifunctional catalase-peroxidases (KatGs) are heme oxidoreductases widely spread among bacteria, archaea and among lower eukaryotes. In fungi, two KatG groups with different localization have evolved, intracellular (KatG1) and extracellular (KatG2) proteins. Here, the cloning, expression analysis and subcellular localization of two novel katG1 genes from the soil fungi Chaetomium globosum and Chaetomium cochliodes are reported. Whereas, the metalloenzyme from Ch. globosum is expressed constitutively, Ch. cochliodes KatG1 reveals a slight increase in expression after induction of oxidative stress by cadmium ions and hydrogen peroxide. The intronless open reading frames of both Sordariomycetes katG1 genes as well as of almost all fungal katG1s possess two peroxisomal targeting signals (PTS1 and PTS2). Peroxisomal localization of intracellular eukaryotic catalase-peroxidases was verified by organelle separation and immunofluorescence microscopy. Co-localization with the peroxisomal enzyme 3-ketoacyl-CoA-thiolase was demonstrated for KatGs from Magnaporthe grisea, Chaetomium globosum and Chaetomium cochliodes. The physiological role of fungal catalase-peroxidases is discussed.Electronic supplementary materialThe online version of this article (doi:10.1007/s00203-013-0887-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Intracellular targeting of ascomycetous catalase-peroxidases (KatG1s)

et al. 2013

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“…The remaining proteins that were more abundant in ejaculated spermatozoa have a variety of metabolic roles including β-oxidation of fatty acids (HADHB, ACADM) , and participation in the TCA cycle (CS) . Other interesting functions include involvement in capacitation (GPD2) and demonstrated antifungal properties (ACAA2) . These varied roles both highlight the complexity of the interaction between spermatozoa and seminal plasma proteins and call for further investigation into their usefulness in improving artificial breeding.…”

Section: Discussionmentioning

confidence: 99%

“…109 Other interesting functions include involvement in capacitation (GPD2) 110 and demonstrated antifungal properties (ACAA2). 111 These varied roles both highlight the complexity of the interaction between spermatozoa and seminal plasma proteins and call for further investigation into their usefulness in improving artificial breeding.…”

Section: Do Seminal Plasma Proteins Prepare Spermatozoa For Cervical ...mentioning

confidence: 99%

Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma

et al. 2016

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Sperm proteomes have emerged for several species; however, the extent of species similarity is unknown. Sheep are an important agricultural species for which a comprehensive sperm proteome has not been produced. In addition, potential proteomic factors from seminal plasma that may contribute to improved fertility after cervical insemination are yet to be explored. Here we use liquid chromatography-tandem mass spectrometry to investigate the proteome of ejaculated ram spermatozoa, with quantitative comparison to epididymal spermatozoa. We also present a comparison to published proteomes of five other species. We identified 685 proteins in ejaculated ram spermatozoa, with the most abundant proteins involved in metabolic pathways. Only 5% of ram sperm proteins were not detected in other species, which suggest highly conserved structures and pathways. Of the proteins present in both epididymal and ejaculated ram spermatozoa, 7% were more abundant in ejaculated spermatozoa. Only two membrane-bound proteins were detected solely in ejaculated sperm lysates: liver enriched gene 1 (LEG1/C6orf58) and epidermal growth factor-like repeats and discoidin I-like domains 3 (EDIL3). This is the first evidence that despite its relatively complex proteomic composition, seminal plasma exposure leads to few novel proteins binding tightly to the ram sperm plasma membrane.

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“…For the fourth step in fatty acid β-oxidation, a 3-ketoacyl-CoA-thiolase (KCT) is required. Peroxisomal isoforms contain either a PTS1 or a PTS2 (Carrie et al, 2007 ; Lee et al, 2009 ; Gonzalez et al, 2011 ), and mitochondrial isoforms can also be integrated in the trifunctional enzyme combining steps 2 to 4 (Camões et al, 2015 ). Our bioinformatic analyses revealed a putative mitochondrial Gt_KCT (ID_159886) with no PTS that is homologous to a subunit of a trifunctional enzyme.…”

Section: Resultsmentioning

confidence: 99%

Inferred Subcellular Localization of Peroxisomal Matrix Proteins of Guillardia theta Suggests an Important Role of Peroxisomes in Cryptophytes

Vasilev

Mix²,

Heimerl³

et al. 2022

Front. Plant Sci.

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Peroxisomes participate in several important metabolic processes in eukaryotic cells, such as the detoxification of reactive oxygen species (ROS) or the degradation of fatty acids by β-oxidation. Recently, the presence of peroxisomes in the cryptophyte Guillardia theta and other “chromalveolates” was revealed by identifying proteins for peroxisomal biogenesis. Here, we investigated the subcellular localization of candidate proteins of G. theta in the diatom Phaeodactylum tricornutum, either possessing a putative peroxisomal targeting signal type 1 (PTS1) sequence or factors lacking a peroxisomal targeting signal but known to be involved in β-oxidation. Our results indicate important contributions of the peroxisomes of G. theta to the carbohydrate, ether phospholipid, nucleotide, vitamin K, ROS, amino acid, and amine metabolisms. Moreover, our results suggest that in contrast to many other organisms, the peroxisomes of G. theta are not involved in the β-oxidation of fatty acids, which exclusively seems to occur in the cryptophyte's mitochondria.

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Antifungal activity of Saccharomyces cerevisiae peroxisomal 3-ketoacyl-CoA thiolase

Cited by 7 publications

References 22 publications

Intracellular targeting of ascomycetous catalase-peroxidases (KatG1s)

Intracellular targeting of ascomycetous catalase-peroxidases (KatG1s)

Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma

Inferred Subcellular Localization of Peroxisomal Matrix Proteins of Guillardia theta Suggests an Important Role of Peroxisomes in Cryptophytes

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