Antifungal activity of proteolytic fraction (P1G10) from (Vasconcellea cundinamarcensis) latex inhibit cell growth and cell wall integrity in Botrytis cinerea

Torres-Ossandón, Maria José; Vega‐Gálvez, Antonio; Salas, Carlos; Rubio, Julia; Silva-Moreno, Evelyn; Castillo, Luís

doi:10.1016/j.ijfoodmicro.2018.08.027

Cited by 22 publications

(24 citation statements)

References 49 publications

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“…In addition, the expression of Bchex , which encodes the principle protein of the woronin body, was markedly down-regulated upon 6-BAP treatment. The woronin body is known to be involved in fungal membrane integrity (38).…”

Section: Resultsmentioning

confidence: 99%

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Cytokinin inhibits fungal development and virulence by targeting the cytoskeleton and cellular trafficking

Gupta

Anand

Pizarro

et al. 2020

Preprint

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Cytokinin (CK) is an important plant developmental regulator, having activities in many aspects of plant life and its response to the environment. CKs are involved in diverse processes in the plant, including stem-cell maintenance, vascular differentiation, growth and branching of roots and shoots, leaf senescence, nutrient balance and stress tolerance. In some cases, phytopathogens secrete CKs. It has been suggested that to achieve pathogenesis in the host, CK-secreting biotrophs manipulate CK signaling to regulate the host cell cycle and nutrient allocation. CK is known to induce host plant resistance to several classes of phytopathogens from a handful of works, with induced host immunity via salicylic acid signaling suggested to be the prevalent mechanism for this host resistance. Here, we show that CK directly inhibits the growth, development, and virulence of fungal phytopathogens. Focusing on Botrytis cinerea (Bc), we demonstrate that various aspects of fungal development can be reversibly inhibited by physiological concentrations of CK. We also found that CK affects both budding and fission yeast in a similar manner. Investigating the mechanism by which CK influences fungal development, we found that CK affects cellular trafficking in Bc, lowering endocytic rates and endomembrane compartment sizes, likely leading to reduced growth rates and arrested developmental programs. Mutant analyses in yeast confirmed that the endocytic pathway is a CK target. Our work uncovers a remarkably conserved role for a plant growth hormone in fungal biology, suggesting that co-evolution between pathogen and host resulted in fascinating molecular adaptations on fundamental processes in eukaryotic biology.

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Section: Resultsmentioning

confidence: 99%

“…The total RNA was isolated using Tri reagent (Sigma-Aldrich) according to the manufacturer's instructions. RNA (3μg) was used to prepare cDNA using reverse transcriptase (Promega, and BcXyn11C (Xylanases) (38,67,75). The primer sequences for each gene detailed in Supplementary Table 2.…”

Section: B Cinerea Virulence Assessmentmentioning

confidence: 99%

Cytokinin inhibits fungal development and virulence by targeting the cytoskeleton and cellular trafficking

Gupta

Anand

Pizarro

et al. 2020

Preprint

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“…The presence of various types of proteases is central to the physiology of GI tract and their expression is increased during IBD, in association with the inflammatory process [32][33][34] . In this scenario, the role of P1G10 can be theorized assuming that plant proteases display substrate specificity oriented to a defensive role in latex containing plants 35 www.nature.com/scientificreports www.nature.com/scientificreports/ host inflammatory proteases for their cognate substrates. During pyloric ligation, P1G10 inhibited pepsin activity by 69% in gastric juice 7 .…”

Section: Discussionmentioning

confidence: 99%

The Proteolytic Fraction From Vasconcellea cundinamarcensis Latex Displays Anti-Inflammatory Effect in A Mouse Model of Acute TNBS-Induced Colitis

Albuquerque

Pizzitola

Silva

et al. 2020

Sci Rep

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The proteolytic fraction (P1G10) from Vasconcellea cundinamarcensis, displays gastric protective and healing activities in different skin lesions in mice and human. In an excisional model, this fraction accelerates resolution of lesions and modulates inflammatory mediators. Based on these data, we assessed its anti-inflammatory activity in murine colitis model, induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) adopted by its physiopathological similarity with human colitis. Twenty four hours after colitis induction followed by three days of treatment, P1G10 at 0.3 and 3.0 mg/Kg induced 30% increase in body weight (p < 0.0001) and ~80% reduction in colon macroscopic damage score (p < 0.05) compared to the untreated TNBS-induced colitis group. Histological analyses showed that 0.3 mg/Kg P1G10 reduced the inflammatory profile and tissue damage (47%, p < 0.05) when it was proteolytically active. Compared to TNBS group, 0.3 mg/Kg P1G10 reduced MPO activity (80%, p < 0.01), MCP-1 (47%, p < 0.05) and TNF-α (50%, no significant) and increased IL-10 (330%, p < 0.001) levels in the supernatant of colonic tissue homogenate. P1G10 treatment also reduced COX-2 expression (60%, p < 0.05) and metalloprotease-2 activity (39%, p < 0.05) while increased globet cell density (140%, p < 0.01), that contributes to mucus layer protection in colonic tissue. Taken together, these findings suggest that low doses of active P1G10 promotes lesion resolution, at least in part by its anti-inflammatory activity, in TNBS-colitis model.

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“…It infects stems, leaves, flowers and fruits of plants during their growing, storage and transportation processes [3], causing significant economic losses pre-and post-harvest [4,5]. The infestation is carried out by the pathogenic fungus through direct penetration or wounds, and stimulated under favourable environmental conditions for fungal growth, particularly at low temperatures as well as high humidity [6,7]. It is tough to control B. cinerea because of its wide host range, genetic diversity and survival as sclerotia, mycelia and conidia [8].…”

Section: Introductionmentioning

confidence: 99%

Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea

Jiang

Song

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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The fungal pathogen Botrytis cinerea is the causal agent of devastating gray mold diseases in many economically important fruits, vegetables, and flowers, leading to serious economic losses worldwide. In this study, a novel actinomycete NEAU-LD23T exhibiting antifungal activity against B. cinerea was isolated, and its taxonomic position was evaluated using a polyphasic approach. Based on the genotypic, phenotypic and chemotaxonomic data, it is concluded that the strain represents a novel species within the genus Streptomyces , for which the name Streptomyces botrytidirepellens sp. nov. is proposed. The type strain is NEAU-LD23T (=CCTCC AA 2019029T=DSM 109824T). In addition, strain NEAU-LD23T showed a strong antagonistic effect against B. cinerea (82.6±2.5%) and varying degrees of inhibition on nine other phytopathogenic fungi. Both cell-free filtrate and methanol extract of mycelia of strain NEAU-LD23T significantly inhibited mycelial growth of B. cinerea. To preliminarily explore the antifungal mechanisms, the genome of strain NEAU-LD23T was sequenced and analyzed. AntiSMASH analysis led to the identification of several gene clusters responsible for the biosynthesis of bioactive secondary metabolites with antifungal activity, including 9-methylstreptimidone, echosides, anisomycin, coelichelin and desferrioxamine B. Overall, this research provided us an excellent strain with considerable potential to use for biological control of tomato gray mold.

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Antifungal activity of proteolytic fraction (P1G10) from (Vasconcellea cundinamarcensis) latex inhibit cell growth and cell wall integrity in Botrytis cinerea

Cited by 22 publications

References 49 publications

Cytokinin inhibits fungal development and virulence by targeting the cytoskeleton and cellular trafficking

Cytokinin inhibits fungal development and virulence by targeting the cytoskeleton and cellular trafficking

The Proteolytic Fraction From Vasconcellea cundinamarcensis Latex Displays Anti-Inflammatory Effect in A Mouse Model of Acute TNBS-Induced Colitis

Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea

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