Antifungal Activity of Lactic Acid Bacteria, Isolated From (Mytilus Galloprovincialis Lam.) in the Bulgarian Black Sea Aquatory

Ibryamova, Sevginar; Arhangelova, Nina; Koynova, Teodora; Dimitrov, Dimitar; Dimitrova, Zheni; Ivanov, Radoslav; Kalchev, K.; Chipev, Nescho; Natchev, Nikolay; Ignatova-Ivanova, Tsveteslava

doi:10.5272/jimab.2020261.2875

Cited by 3 publications

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“…), exhibited antimycotic activity against A. niger. Interesting, this mold species was the most sensitive to inhibitory compounds of marine lactobacteria among the tested Penicillium claviforme, Candida albicans, and Saccharomyces cerevisiae [6]. However, in other publication a low sensitivity of A. niger to LAB (of food origin) was reported [7].…”

Section: Mrs Brothmentioning

confidence: 90%

“…Lactic acid bacteria (LAB) are large group of microorganisms including representatives of various genera such as Lactobacillus, Halolactobacillus, Marinilactobacillus, Pediococcus, Carnobacterium, Enterococcus etc., which inhabit many ecological niches, including marine environment [1]. A great diversity of secondary metabolites including organic acids, bacteriocins, peptides and other compounds, which possess antimycotic activity are produced by lactobacteria [6].…”

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“…It is known about great difference of marine environment compared to other ecological niches, primary by its extreme conditions indicating a possibility of isolation of bacterial strains with high antagonistic activity [2,9]. Lactobacteria from marine environment can exhibit antimycotic activity and they can find possible application in food industry due to it [6].…”

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Antimycotic Activity of the Isolates of Lactobacteria From Wa-Ter and Mussels of Black Sea

Merlich,

Kimurzhyi,

Kovalchuk

et al. 2023

mbt

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Aim. Determination of the presence of antimycotic activity and its level in thirteen lactobacteria isolates from Black Sea water and mussels. Methods. Antimycotic activity of lactic acid bacteria (LAB) isolates was tested by agar diffusion method in 24-well plates. To determine character of action the plates were incubated for 7 days at 25 °C. Results. For the first time antimycotic activity of marine LAB from Odesa coast was estimated. The majority of the tested isolates of lactobateria exhibited antimycotic activity of high level completely inhibiting (in 100%) as mycelium growth as well as spore formation of Penicillium expansum UKM F-575 and Aspergillus niger UKM F-16706 on second day of the study. Four isolates (M.5.1, M.5.2, M.7.1, M.7.2) showed lower antimycotic activity (from 0 to 75%). Eight LAB isolates from seawater (W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4) and one isolate from mussel liquor (M4.1) completely inhibited mycelial growth and sporulation of P. expansum UКМ F-575 and A. niger UКМ F-16706 even within seven days indicating fungicidal character of action. Conclusions. The most of tested LAB isolates from Black Sea exhibited high antimycotic activity against P. expansum UКМ F-575 and A. niger UКМ F-16706 indicator strains. The most active lactobacteria isolates were W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4 isolated from Black Sea water and M4.1 – from mussel liquor. The character of the revealed antimycotic activity was determined as fungicidal.

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Antimycotic Activity of the Isolates of Lactobacteria From Wa-Ter and Mussels of Black Sea

Merlich,

Kimurzhyi,

Kovalchuk

et al. 2023

mbt

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Bioprospecting for Novel Bacterial Sources of Hydrolytic Enzymes and Antimicrobials in the Romanian Littoral Zone of the Black Sea

et al. 2022

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Marine microorganisms have evolved a large variety of metabolites and biochemical processes, providing great opportunities for biotechnologies. In the search for new hydrolytic enzymes and antimicrobial compounds with enhanced characteristics, the current study explored the diversity of cultured and uncultured marine bacteria in Black Sea water from two locations along the Romanian coastline. Microbial cell density in the investigated samples varied between 65 and 12.7 × 103 CFU·mL−1. The total bacterial community identified by Illumina sequencing of 16S rRNA gene comprised 185 genera belonging to 46 classes, mainly Gammaproteobacteria, Alphaproteobacteria, Flavobacteriia, and 24 phyla. The 66 bacterial strains isolated on seawater-based culture media belonged to 33 genera and showed variable growth temperatures, growth rates, and salt tolerance. A great fraction of these strains, including Pseudoalteromonas and Flavobacterium species, produced extracellular proteases, lipases, and carbohydrases, while two strains belonging to the genera Aquimarina and Streptomyces exhibited antimicrobial activity against human pathogenic bacteria. This study led to a broader view on the diversity of microbial communities in the Black Sea, and provided new marine strains with hydrolytic and antimicrobial capabilities that may be exploited in industrial and pharmaceutical applications.

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Determination of the antimicrobial activity of lactic acid bacteria isolated from the Black sea mussel Mytilus galloprovincialis Lamarck, 1819

Ignatova-Ivanova¹,

Ibryamova²,

Bachvarova³

et al. 2022

PHAR

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The present study reports on the determination of the antimicrobial activity of lactic acid bacteria (LAB) isolated from the Black sea mussel Mytilus galloprovincialis Lamarck, 1819. The samples were collected in the period of August 2018 until March 2021. The BIOLOG system was used for microbiological determination. From the mussel M. galloprovincialis Lam. four species of LAB were isolated - Sporolactobacillus kofuensis, Lactobacillus sakei, Streptococcus gallolyticus ss gallolyticus and Lactibacillus brevis. The activity of the strains was determined against test cultures (Escherichia coli 3398, Staphylococus aureus 745, Bacillus subtilis 6633, Salmonella typhimurium 3591, Listeria monocytogens 863 Enterobacter aerogenes 3691, Aspergillus niger, Penicillium claviforme, Saccharomyces cerevisae, Candida albicans 8673 and Candida glabrata 72). Before the analysis for antimicrobial activity, the LAB were cultured in media with different concentrations of sugars - 2, 5 and 10%. The results showed that 4 strains S. kofuensis, L. sakei, S. gallolyticus ss gallolyticus and L. brevis cultured on glucose and oligosaccharides completely lost their activity in all studied variants. Therefore, some carbohydrates (glucose) and oligosaccharides induce the synthesis outside the cell of biologically active molecules, which can probably be attributed to peptides/proteins.

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Antifungal Activity of Lactic Acid Bacteria, Isolated From (Mytilus Galloprovincialis Lam.) in the Bulgarian Black Sea Aquatory

Cited by 3 publications

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Antimycotic Activity of the Isolates of Lactobacteria From Wa-Ter and Mussels of Black Sea

Antimycotic Activity of the Isolates of Lactobacteria From Wa-Ter and Mussels of Black Sea

Bioprospecting for Novel Bacterial Sources of Hydrolytic Enzymes and Antimicrobials in the Romanian Littoral Zone of the Black Sea

Determination of the antimicrobial activity of lactic acid bacteria isolated from the Black sea mussel Mytilus galloprovincialis Lamarck, 1819

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Antifungal Activity of Lactic Acid Bacteria, Isolated From (Mytilus Galloprovincialis Lam.) in the Bulgarian Black Sea Aquatory

Cited by 3 publications

References 0 publications

Antimycotic Activity of the Isolates of Lactobacteria From Wa-Ter and Mussels of Black Sea

Antimycotic Activity of the Isolates of Lactobacteria From Wa-Ter and Mussels of Black Sea

Bioprospecting for Novel Bacterial Sources of Hydrolytic Enzymes and Antimicrobials in the Romanian Littoral Zone of the Black Sea

﻿Determination of the antimicrobial activity of lactic acid bacteria isolated from the Black sea mussel Mytilus galloprovincialis Lamarck, 1819

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Determination of the antimicrobial activity of lactic acid bacteria isolated from the Black sea mussel Mytilus galloprovincialis Lamarck, 1819