Antifungal activity of <i>Mahonia aquifolium</i> extract and its major protoberberine alkaloids

Volleková, A; Kostálová, D; Kettmann, V.; Toth, John P.

doi:10.1002/ptr.1256

Cited by 100 publications

(57 citation statements)

References 8 publications

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“…1). JA was chosen because of its reported attractiveness as the new antifungal agent with very low host toxicity [19]. For comparison, ethidium bromide (ET), the classic DNA intercalator [20], was included to the interaction study, as well.…”

Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoretic studies of interactions of some quaternary isoquinoline alkaloids with DNA constituents and DNA

et al. 2005

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Capillary zone electrophoresis was applied for the investigation of interactions of some quaternary isoquinoline alkaloids, namely sanguinarine, chelerythrine, berberine, and jatrorrhizine, with DNA constituents and with DNA. None of these alkaloids attach covalently to nucleotides or to the whole DNA under physiological conditions. The interaction with DNA constituents is a noncovalent complexation based on weak intermolecular forces. Electrostatic attraction participates in the interaction but other types of intermolecular forces are involved as well. Cations were identified as the most probable interacting forms of the alkaloids. The interaction with compounds derived from purine was always stronger than those derived from pyrimidine. All alkaloids behaved analogously and similarly to ethidium bromide, the classic DNA intercalator. Stability constants K (in l.mol(-1)) for sanguinarine and chelerythrine in phosphate buffer of pH 7.4 (I(S) = 30 mM) ranged from tens to hundreds.

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Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoretic studies of interactions of some quaternary isoquinoline alkaloids with DNA constituents and DNA

et al. 2005

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“…Ber extracts and decoctions have significant antimicrobial activities. Recent pharmacological studies have shown that Ber also possesses antitumor (Kettmann et al, 2004), anti-HIV (Gudima et al, 1994), antifungal (Vollekova et al, 2003), cardioprotective (Zheng et al, 2003), immunoregulative , antimalarial (Tran et al, 2003), anti-inflammatory , antioxidative (Rockova et al, 2004), cerebroprotective (Ma et al, 1999), antimutagenic (Cernakova et al, 2002), vasorelaxing (Ko et al, 2000), anxiolytic (Peng et al, 2004), and analgesic effects. Ber is generally administered as a chloride or sulfate for clinical applications.…”

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confidence: 99%

Pharmacokinetics of Berberine and Its Main Metabolites in Conventional and Pseudo Germ-Free Rats Determined by Liquid Chromatography/Ion Trap Mass Spectrometry

Zuo

Nakamura²,

Akao³

et al. 2006

Drug Metab Dispos

248

153

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ABSTRACT:Berberine (Ber) and its main metabolites were identified and quantified using liquid chromatography/electrospray ionization/ion trap mass spectrometry. Rat plasma contained the main metabolites, berberrubine, thalifendine, demethyleneberberine, and jatrorrhizine, as free and glucuronide conjugates after p.o. Ber administration. Moreover, the original drug, the four main metabolites, and their glucuronide conjugates were all detected in liver tissues after 0.5 h and in bile samples 1 h after p.o. Ber administration. Therefore, the metabolic site seemed to be the liver, and the metabolites and conjugates were evidently excreted into the duodenum as bile. The pharmacokinetics of Ber and the four metabolites were determined in conventional and pseudo germ-free rats (treated with antibiotics) after p.o. administration with 40 mg/kg Ber. The AUC 0-limt and mean transit time values of the metabolites significantly differed between conventional and pseudo germ-free rats. The amounts of metabolites were remarkably reduced in the pseudo germ-free rats, whereas levels of Ber did not obviously differ between the two groups. The intestinal flora did not exert significant metabolic activity against Ber and its metabolites, but it played a significant role in the enterohepatic circulation of metabolites. In this sense, the liver and intestinal bacteria participate in the metabolism and disposition of Ber in vivo.

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“…However, contradictory results of either synergic or antagonistic actions in various antifungal combinations have been reported (8,11,16,27). As for fluconazoleresistant C. albicans, few data are available about the synergism of fluconazole with other antifungal agents (1,4,25).Berberine, a bioactive herbal ingredient, was demonstrated to have weak activity against C. albicans and C. glabrata (17,21,26). Recently, berberine was combined with amphotericin B to treat disseminated candidiasis in mice (10).…”

mentioning

confidence: 99%

“…Berberine, a bioactive herbal ingredient, was demonstrated to have weak activity against C. albicans and C. glabrata (17,21,26). Recently, berberine was combined with amphotericin B to treat disseminated candidiasis in mice (10).…”

mentioning

confidence: 99%

Potent In Vitro Synergism of Fluconazole and Berberine Chloride against Clinical Isolates of Candida albicans Resistant to Fluconazole

Quan

Cao

et al. 2006

Antimicrob Agents Chemother

139

136

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In vitro interaction of fluconazole and berberine chloride was investigated against 40 fluconazole-resistant clinical isolates of Candida albicans. Synergism in fungistatic activity was found with the checkerboard microdilution assay. The findings of agar diffusion tests and time-kill curves confirmed the synergistic interaction, but no antagonistic action was observed.Candida albicans is the most common candidal pathogen, causing mucosal and invasive infections (7,9,23,29). With the increasing clinical use of fluconazole, a choice for the treatment of C. albicans infections (3, 5, 12), fluconazole-resistant isolates are occurring more frequently (2,19,28). Attempts have been made to cope with treatment failures by using combination therapy (14). However, contradictory results of either synergic or antagonistic actions in various antifungal combinations have been reported (8,11,16,27). As for fluconazoleresistant C. albicans, few data are available about the synergism of fluconazole with other antifungal agents (1,4,25).Berberine, a bioactive herbal ingredient, was demonstrated to have weak activity against C. albicans and C. glabrata (17,21,26). Recently, berberine was combined with amphotericin B to treat disseminated candidiasis in mice (10). To seek a novel combination therapy, we investigated the in vitro interaction of fluconazole and berberine chloride (BBR) against fluconazoleresistant clinical isolates of C. albicans.Strains and agents. Forty clinical isolates of fluconazoleresistant C. albicans were used in this study, and C. albicans ATCC 90028 was used as a quality control. Drugs prepared in dimethyl sulfoxide (DMSO) included fluconazole (PfizerRoerig Pharmaceuticals, New York, NY) and BBR (SigmaAldrich, St. Louis, MO).Checkerboard microdilution assay. Assays were performed on all 40 isolates according to methods of the CLSI (formerly NCCLS) (M27-A) (6, 18). The initial concentration of fungal suspension in RPMI 1640 medium was 10 3 CFU/ml, and the final concentrations ranged from 0.125 to 64 g/ml for fluconazole and 1 to 32 g/ml for BBR. Plates were incubated at 35°C for 24 h. Optical density was measured at 630 nm, and background optical densities were subtracted from that of each well. Each isolate was tested in triplicate. MIC 80 and MIC 50 were determined as the lowest concentration of the drugs (alone or in combination) that inhibited growth by 80% or 50%, respectively, compared with that of drug-free wells. The fractional inhibitory concentration (FIC) index is defined as the sum of the MIC of each drug when used in combination divided by the MIC of the drug used alone. Synergy and antagonism were defined by FIC indices of Յ0.5 and Ͼ4, respectively. An FIC index result of Ͼ0.5 but Յ4 was considered indifferent (20).Agar diffusion test. C. albicans 0304103 (one fluconazole-resistant isolate with a MIC of Ͼ32 g/ml for BBR) was tested by agar diffusion assay. A 100-l aliquot of 10 6 -CFU/ml suspension was spread uniformly onto the yeast extract-peptone-dextrose agar plate with or without 64 g/ml ...

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Antifungal activity of Mahonia aquifolium extract and its major protoberberine alkaloids

Cited by 100 publications

References 8 publications

Capillary zone electrophoretic studies of interactions of some quaternary isoquinoline alkaloids with DNA constituents and DNA

Capillary zone electrophoretic studies of interactions of some quaternary isoquinoline alkaloids with DNA constituents and DNA

Pharmacokinetics of Berberine and Its Main Metabolites in Conventional and Pseudo Germ-Free Rats Determined by Liquid Chromatography/Ion Trap Mass Spectrometry

Potent In Vitro Synergism of Fluconazole and Berberine Chloride against Clinical Isolates of Candida albicans Resistant to Fluconazole

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