Antifungal activity of five different essential oils in vapour phase for the control of <i>Colletotrichum gloeosporioides</i> and <i>Lasiodiplodia theobromae in vitro</i> and on mango

Perumal, Anand Babu; Sellamuthu, Periyar Selvam; Nambiar, Reshma B.; Sadiku, Rotimi

doi:10.1111/ijfs.12991

Cited by 76 publications

(50 citation statements)

References 36 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…) and Colletotrichum gloeosporioides (Perumal et al . ). Several studies have investigated the antifungal activity of EOs against postharvest disease in fruits such as citrus (Kouassi et al .…”

Section: Introductionmentioning

confidence: 97%

Carvacrol and eugenol effectively inhibitRhizopus stoloniferand control postharvest soft rot decay in peaches

Zhou

Wang

et al. 2017

J Appl Microbiol

View full text Add to dashboard Cite

Aims: This study aimed to investigate the antifungal mechanism of carvacrol and eugenol to inhibit Rhizopus stolonifer and the control of postharvest soft rot decay in peaches. Methods and Results: To investigate the antifungal mechanism, the effects of carvacrol and eugenol on the mycelium growth, leakages of cytoplasmic contents, mycelium morphology, cell membrane and membrane composition of R. stolonifer were studied. Carvacrol and eugenol both exhibited dosedependent antifungal activity against R. stolonifer, carvacrol at a concentration of 2 ll per plant and eugenol at a concentration of 4 ll per plant inhibited fungal growth completely. The two essential oils (EOs) increased cell membrane penetrability and caused the leakage of cytoplasm, nucleic acid and protein content. The observation using scanning electron microscopy and fluorescent microscopy showed modification of the hyphal morphology and breakage of the cell plasma membrane. Decreased ergosterol contents confirmed that the two EOs could destroy the membrane of R. stolonifer. For the in vivo test, the inhibition of soft rot disease and the induction of defencerelated enzymes were investigated. Carvacrol and eugenol significantly reduced the incidence and severity of soft rot decay in inoculated peaches. The best treatments for controlling soft rot decay were obtained at 0Á5 ll l À1 for carvacrol and 1 ll l À1 for eugenol. The activities of defence-related enzymes in peaches were also enhanced by fumigation with two EOs. Conclusion: This study showed that carvacrol and eugenol could effectively inhibit the growth of R. stolonifer in vitro and successfully control the incidence of soft rot decay in honey peaches. Significance and Impact of the Study: The above findings may be the main antifungal mechanism of carvacrol and eugenol on R. stolonifer. Furthermore, carvacrol and eugenol are helpful for their commercial application on the preservation of fresh fruit.

show abstract

Section: Introductionmentioning

confidence: 97%

Carvacrol and eugenol effectively inhibitRhizopus stoloniferand control postharvest soft rot decay in peaches

Zhou

Wang

et al. 2017

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…Endophytically, these fungi live asymptomatically and occasionally symptomatically within the healthy tissue of the host (Faeth & Fagan, ). In South Africa, L. theobromae has been recognized as one of the species of Botryosphaeriaceae that affects avocado (Darvas & Kotzé, ) and other subtropical fruits such as mango (Perumal, Sellamuthu, Nambiar, & Sadiku, ; Syed, Mansha, Khaskheli, Khanzada, & Lodhi, ), banana (Nath, Solanky, Mahatma, & Madhubala, ; Twumasi, Ohene‐Mensa, & Moses, ), papaya (Cavalcante et al., ; Netto et al., ) and citrus (Zhang et al, ; Zhao, Bai, McCollum, & Baldwin, ).…”

Section: Introductionmentioning

confidence: 99%

Natural plant volatiles as an alternative approach to control stem‐end rot in avocado cultivars

Obianom

Sivakumar

2017

Journal of Phytopathology

View full text Add to dashboard Cite

Stem-end rot is a postharvest disease associated with multiple important fungal pathogens including Lasiodiplodia theobromae. The incidence of stem-end rot in avocado during postharvest storage affects the shelf life, quality and marketability of the fruit.This study is aimed at the investigation of the antifungal activities of selected natural plant volatiles (vapour phase): citral, octanal, hexanal and thymol against L. theobromae (causal pathogen of stem-end rot) in vitro and in vivo in "Hass" and "Fuerte" avocados.Hexanal showed a lower inhibitory effect on the radial mycelial growth of L. theobromae in vitro. However, citral at a minimum concentration of 4 μl/L revealed fungicidal activity and completely inhibited the spore germination of L. theobromae. Artificially inoculated "Hass" and "Fuerte" avocados with L. theobromae were exposed to citral (768 μl) and commercial fungicide prochloraz and stored for 6 days at 20°C and 14 days at 10°C separately and thereafter held at 20°C for 3 days to simulate the retail shelf conditions. Although citral in a volatile phase effectively reduced the development of stem-end rot in both cultivars, its effect was significant in "Fuerte" with 75% reduction in the incidence of stem-end rot. The biochemical analysis demonstrated an increase in total phenol contents, phenylalanine ammonia-lyase, chitinase and β-1, 3 glucanase activity in fruit exposed to citral when compared to the reference treatment prochloraz and the untreated control for both cultivars. Furthermore, fruits exposed to citral retained the ready-to-eat firmness and therefore could be considered a potential alternative treatment to control stem-end rot at the postharvest stage. K E Y W O R D Schitinase, citral, Lasiodiplodia theobromae, phenols, phenylalanine ammonia-lyase | INTRODUCTIONAvocado (Persea americana Mill.) is predominantly recognized as a rich source of monounsaturated fatty acids and other important vitamins, which are beneficial to human health and nutrition (Donetti & Terry, 2014;Dreher & Davenport, 2013;Ferreyra et al., 2016). Avocado has been reported to possess a reasonable number of phenolic compounds that play an important role in the defence mechanisms of the fruit against potential pathogenic fungi. The phenolic content in avocado contributes to about 60 mg and 40 mg gallic acid equivalents for each 30 g and one-half fruit, respectively (Dreher & Davenport, 2013).Globally, the increase in postharvest losses is attributed to the fruit susceptibility to several fungal attacks, which impacts on the shelf life and commercial value of the fruit during the supply chain (Sivakumar & Bautista-Banos, 2014;Suvama, Hemalatha, Reddy, & Reddy, 2015). | MATERIAL AND METHODS | Isolation of the pathogenL. theobromae was isolated from symptomatic avocado ("Hass" and "Fuerte") fruits and maintained on a potato dextrose agar (PDA) at 30°C for 7 days. The spore suspension was obtained from a viable 7-day-old culture by adding sterile distilled water and adjusting the suspension with a haemocytomet...

show abstract

“…The size of the clear zone increased with increasing HBEO concentrations. Previous studies have shown that antimicrobial capability depends largely on oil concentration (Viuda-Martos et al, 2008;Perumal et al, 2016;Cosmai et al, 2017;Yang et al, 2017). Further increasing the GEO concentration was not considered as at high concentrations the odour may affect sensory aroma acceptance.…”

Section: Effects Of Increased Geo or Hbeo Concentrations In A Vapour mentioning

confidence: 99%

“…The disc diffusion method, however, may present a problem, as EOs are hydrophobic, and volatile components have low water solubility in media (Nedorostova et al, 2009;Tajkarimi et al, 2010). In contrast, the volatilisation method is considered suitable (Nedorostova et al, 2009;Ali et al, 2015;Perumal et al, 2016;Shahabi et al, 2017) for determining antimicrobial activity and obtaining minimal inhibitory concentration (MIC) or minimum bactericidal concentration (MBC), which represents the lowest concentration of EO required to inhibit target microorganisms (Dannenberg et al, 2016;Lu et al, 2016;Alejo-Armijo et al, 2017). The screening method is not standardised for comparing liquid and vapour phases of EO (Tyagi & Malik, 2011;Boukhatem et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobia activity of garlic (Allium sativum L.) and holy basil (Ocimum sanctum L.) essential oils applied by liquid vs. vapour phases

Torpol

Wiriyacharee

Sriwattana

et al. 2018

Int J of Food Sci Tech

View full text Add to dashboard Cite

Summary Antimicrobial activity of garlic (Allium sativum L.) and holy basil (Ocimum sanctum L.) essential oils (GEO and HBEO, respectively) was evaluated in liquid vs. vapour phases. Diallyl disulphide and diallyl trisulphide in GEO while eugenol, caryophyllene and methyl eugenol in HBEO were predominant active compounds. Individually, GEO was highly effective in the vapour phase, showing the greatest antimicrobial activity (P ≤ 0.05) against Bacillus cereus and Staphylococcus aureus with complete inhibition, followed by Salmonella typhimurium and Pseudomonas fluorescens, but had no effect on Escherichia coli, Lactobacillus plantarum or Listeria monocytogenes. The combined GEO:HBEO (a 1:1 ratio) at 30 μL demonstrated inhibitive activity against all bacteria tested with complete inhibition against B. cereus and S. aureus. Minimum bactericidal concentration (MBC) of the combined oil was <0.4% v/v, except for P. fluorescens requiring a higher MBC (1.5%, v/v). This study demonstrated potential of the combined GEO:HBEO to be used for food preservation applications.

show abstract

Antifungal activity of five different essential oils in vapour phase for the control of Colletotrichum gloeosporioides and Lasiodiplodia theobromae in vitro and on mango

Cited by 76 publications

References 36 publications

Carvacrol and eugenol effectively inhibitRhizopus stoloniferand control postharvest soft rot decay in peaches

Carvacrol and eugenol effectively inhibitRhizopus stoloniferand control postharvest soft rot decay in peaches

Natural plant volatiles as an alternative approach to control stem‐end rot in avocado cultivars

Antimicrobia activity of garlic (Allium sativum L.) and holy basil (Ocimum sanctum L.) essential oils applied by liquid vs. vapour phases

Contact Info

Product

Resources

About