Antifungal activity of extracts from medicinal plants used by First Nations Peoples of eastern Canada

Jones, N; Arnason, John T.; Abou-Zaid, Mamdouh M.; Akpagana, Koffi; Sánchez-Vindas, Pablo; Smith, Myron L.

doi:10.1016/s0378-8741(00)00306-8

Cited by 65 publications

(52 citation statements)

References 9 publications

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“…The other fungal strains tested during the present study were completely resistant to different extracts at all concentrations showing no zone of inhibition. The results agree with Bergeron et al (1996), and Jones et al (2000), Prince andPrabakaran (2011), Bakht et al, (2013 a,b).…”

Section: Discussionsupporting

confidence: 89%

Antimicrobial and anti-oxidant potential of Periploca hydaspidis

Ullah

Bakht

Shafi

2015

Bangladesh J Pharmacol

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The present study investigates antimicrobial and anti-oxidant potential of different solvent extracted samples from Periploca hydaspidis through disc diffusion assay. The data revealed that all the extracts reduced the growth of the tested microbes using three different concentrations. Pseudomonas aeruginosa and E. coli were sensitive to crude extracts and all fractions measuring varying degree of growth inhibition. Similarly, the growth of Citrobacter freundii was not inhibited by crude methanolic extracts, n-hexane, ethyl acetate and aqueous extracted samples. Maximum growth inhibition was measured against Klebsiella pneumoniae exposed to aqueous fraction followed by n-hexane fraction. Our results also suggested that among various fungi, Candida albicans was sensitive to crude methanolic extracts, n-hexane and aqueous fractions compared with other fractions. The rest of the fungi under test were resistant to crude and all fractions showing no zone of inhibition. All the extracted samples of the stems and roots showed antioxidant activity, however, crude methanolic extract of stem tissues exhibited better antioxidant activity than the other extracts.

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Section: Discussionsupporting

confidence: 89%

Antimicrobial and anti-oxidant potential of Periploca hydaspidis

Ullah

Bakht

Shafi

2015

Bangladesh J Pharmacol

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“…The ability of the stem bark extracts to prevent infections is one of the basis of its use in wound healing, as infected wounds takes a longer time to heal. The bacteria strains chosen for the study, namely: B. subtilis, S. aureus, E. coli, and P. aeruginosa, are commonly found in infected wounds (Jones et al, 2000) while C. albicans is a known human opportunistic pathogen (Mensah et al, 2004). Activity of S. campanulata extract against these organisms and others implicated in wound infections would confirm the wound healing property of the plant and validate its use in folklore medicine.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of extracts and topical products of the stem bark of Spathodea campanulata for wound healing

Ofori-Kwakye

Kwapong²,

Adu³

2010

Afr. J. Trad. Compl. Alt. Med.

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The antibacterial activity of the aqueous, ethanol, methanol and petroleum ether Soxhlet extracts of sundried stem bark of Spathodea campanulata P. Beauv. (Bignoniaceae) was investigated by testing the extracts against B. subtilis, E. coli, P. aeruginosa and S. aureus. The minimum inhibitory concentration (MIC) of the methanol extract was determined against the four bacteria strains and C. albicans using the broth dilution method. Four topical products were prepared by incorporating the methanol extract of S. campanulata (20 % w/w) into aqueous cream, soft paraffin, emulsifying ointment and simple ointment bases and evaluated for their in vitro antimicrobial efficacy. The effect of storage time on the activity of the methanol extract of S. campanulata and S. campanulata extract incorporated in aqueous cream base was also investigated. The methanol and ethanol extracts showed good activity while the aqueous and petroleum ether extracts exhibited little activity. The methanol extract showed the best antibacterial activity. The MIC of the methanol extract of S. campanulata was: C. albicans (45 -50 mg/ml), B. subtilis and E. coli (50 -55 mg/ml), P. aeruginosa (60 -65 mg/ml), S. aureus (145 -150 mg/ml). Antimicrobial activity of S. campanulata in the topical bases was in the order: aqueous cream > emulsifying ointment > simple ointment > white soft paraffin. Antimicrobial activity of S. campanulata in aqueous cream decreased (p < 0.05) upon storage at room temperature for 6-months. The antifungal activity of the methanol extract of S. campanulata was reduced (p < 0.05) upon storage while antibacterial activity was largely unaffected.

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“…Chimaphilin (2,7-dimethyl-1,4-naphthoquinone) is an antifungal compound found in pipsissewa (C. umbellata), a plant used by First Nations Peoples of eastern Canada to treat infections (Arnason et al, 1981;Jones et al, 2000). Previously, a GDA analysis revealed that mutations in genes involved in mRNA transcription and cell wall functions were prevalent among those strains most sensitive to chimaphilin (Galván et al, 2007).…”

Section: Reporter Gene Expression Assay To Test Effects On Fungal Tramentioning

confidence: 99%

“…Traditional healers have long recognized antiseptic properties of specific plant species, and this knowledge base serves as a useful starting point in the discovery process. For example, in a study by Arnason's group (Jones et al, 2000), it was found that plants used most often for treating infection-like symptoms by First Nations Peoples of eastern Canada had higher overall antifungal activities than medicinal plants used for other purposes.…”

Section: Introductionmentioning

confidence: 99%

Secondary Assays for Testing the Mode of Action of Natural Products with Bioactivity Against Fungi

Smith

Cruz

Golshani

et al. 2008

Pharmaceutical Biology

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Identification of novel antifungals is needed, and plantderived compounds offer important leads in this respect. This article initiates a compendium of secondary assays for testing hypotheses on mode of action of plant-derived compounds. Three assays were developed to evaluate compounds that are hypothesized to effect cell wall function, transcription, and oxidative stress. A hypothesized perturbation on cell wall integrity by chimaphilin and Echinacea root extracts was supported using sonication and heat shock assays. Heat shock treatments reduced the number of viable cells recovered when yeast was grown in the presence of subinhibitory concentrations of chimaphilin. A reduction in cell numbers was also observed with both sonicationbased and heat shock-based assays when yeast was grown in the presence of Echinacea purpurea (L.) Moench (Asteraceae) extracts. The hypothesis that chimaphilin impedes mRNA transcription processes was also supported by a β-galactosidase reporter gene assay. The antioxidant activity of nordihydroguaiaretic acid (NDGA) and ascorbic acid were assessed using a yeast strain that does not grow under hyperoxic conditions. At the concentrations assayed, NDGA did not remediate this sensitivity to hyperoxia, whereas ascorbic acid did. These and additional assays are further reviewed with an aim to collate and standardize secondary assays and streamline antifungal mode of action determinations.

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Antifungal activity of extracts from medicinal plants used by First Nations Peoples of eastern Canada

Cited by 65 publications

References 9 publications

Antimicrobial and anti-oxidant potential of <i>Periploca hydaspidis</i>

Antimicrobial and anti-oxidant potential of <i>Periploca hydaspidis</i>

Antimicrobial activity of extracts and topical products of the stem bark of <i>Spathodea campanulata</i> for wound healing

Secondary Assays for Testing the Mode of Action of Natural Products with Bioactivity Against Fungi

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