Antifungal Activity of Condensed Tannins from Stryphnodendron adstringens: Effect on Candida tropicalis Growth and Adhesion Properties

Abstract

Candida species are some of the most common causes of fungal infection worldwide. The limited efficacy of clinically available antifungals warrants the search for new compounds for treating candidiasis. This study evaluated the effect of condensed tannin-rich fraction (F2 fraction) of Stryphnodendron adstringens on in vitro and in vivo growth of Candida tropicalis, and on yeast adhesion properties. F2 exhibited a fungistatic effect with the minimum inhibitory concentration ranging from 0.5 to 8.0 μg/mL. A significant reduction in biofilm mass was observed after either pretreatment of planktonic cells for 2 h (mean reduction of 46.31±8.17%) or incubation during biofilm formation (mean reduction of 28.44±13.38%) with 4x MIC of F2. Prior exposure of planktonic cells to this F2 concentration also significantly decreased yeast adherence on HEp-2 cells (mean reduction of 43.13±14.29%), cell surface hydrophobicity (mean reduction of 25.89±10.49%) and mRNA levels of the genes ALST1-3 (2.9-, 1.8- and 1.8-fold decrease, respectively). Tenebrio molitor larvae, which are susceptible to C. tropicalis infection, were used for in vivo testing. Treatment with 128 and 256 μg/mL F2 significantly increased the survival of infected larvae. These results indicate a combined effect of F2 on inhibition of yeast growth and interference in yeast adhesion, which may contribute to the suppression of infection caused by C. tropicalis, thus reinforcing the potential of the condensed tannins from S. adstringens for the development of novel antifungal agents.

Anti-yeast potential of some Annonaceae species from Cameroonian biodiversity

“…Also, flavonoids used in combination with fluconazole recently showed significant synergistic activity in vitro against Candida tropicalis strains resistant to fluconazole (Da Silva et al, 2014). Similarly, condensed tannins from Stryphnodendron adstringens showed in vitro and in vivo effect on Candida tropicalis growth and adhesion properties (Morey et al, 2016). Steroidal saponins have also been shown to exert significant activity against Cr.…”

Evaluation of In Vitro Antioxidant and Anticancer Properties of the Aqueous Extract from the Stem Bark of Stryphnodendron adstringens

“…Stryphnodendron adstringens is one of the species found in the Brazilian Cerrado that shows potential for the development of new drugs considering its chemical composition and biological activities previously described in the literature [3,5,6]. Previous phytochemical studies show that S. adstringens stem bark has proanthocyanidins [7,32], chalcones and triterpene compounds [4].…”

“…In traditional medicine, decoctions or infusions of the stem bark of this plant are used to treat prostate problems, liver and skin diseases, poor circulation, wounds, fungal infections, dental inflammation, gastritis and diabetes [1,2]. Some of these effects have already been scientifically shown, including their wound healing [3], anti-inflammatory [4,5], antifungal [6,7] activities. However, other biological properties may still be characterized.…”

Pharmaceutical topical gel containing proanthocyanidin polymers-rich fraction from Stryphnodendron adstringens

“…Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License Glehn and Rodrigues, 2012;Hernandes et al, 2010;Herzog-soares and Alves, 2002;Ishida et al, 2009;Lanchoti Fiori et al, 2013;Luize et al, 2005;Morey et al, 2016;Pinto et al, 2015). In addition, in Brazil there is a commercialized pharmaceutical ointment named Fitoscar ® containing S. adstringens extract for woundhealing.…”