Antifungal activity of Bacillus subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight of marigold

Priyanka, R.; Nakkeeran, S.; Pravin, I. Arumuka; Moorthy, A. S. Krishna; Sivakumar, U.

doi:10.18311/jbc/2018/21134

Cited by 8 publications

(6 citation statements)

References 11 publications

(12 reference statements)

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“…In general, plants applied with B. subtilis influenced plant growth promotion, which showed maximum plant growth promotion in Gloriosa. Similarly type of results was observed by Priyanka et al (2018) who found that foliar application B. subtilis subsp. spizizenii (MM19) effectively suppressed the leaf blight of marigold caused by A. alternata under field conditions up to 77 per cent.…”

Section: Resultssupporting

confidence: 78%

Strategies for the Management of Leaf Blight Disease Caused by Alternaria alternata in Gloriosa superba (L.)

Meena¹,

Rajamanickam²,

P³

et al. 2021

MAJ

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Leaf blight caused by Alternaria alternata (Fr.) Keissler is a severe disease of Gloriosa, causing severe yield loss in every part of Gloriosa growing areas of Tamil Nadu. The foliar pathogen was isolated from disease-infected leaves and proved its pathogenicity. Further, the molecular analysis of the pathogen using 18S rDNA confirmed the pathogen as Alternaria alternata. Attempts were made to explore the biocontrol agents and fungicides for the management of leaf blight incidence under field conditions. Four field trials conducted from 2014 to 2018 revealed that foliar application of talc-based formulation of Bacillus subtilis significantly reduced the leaf blight incidence and increased the seed yield under field conditions. Besides, prophylactic application of these biocontrol agents has also increased the plant growth parameters like plant height, number of flowers/plant, no. of pods/plant and number of seeds/pod. Similarly, foliar application of chlorothalonil (0.1%) and mancozeb (0.2%) was also credited to managing the leaf blight disease under field conditions.

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Section: Resultssupporting

confidence: 78%

Strategies for the Management of Leaf Blight Disease Caused by Alternaria alternata in Gloriosa superba (L.)

Meena¹,

Rajamanickam²,

P³

et al. 2021

MAJ

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“…In 2018, Priyanka proved that the mycelial growth of A. alternata, which causes crop disease, was inhibited by B. subtilis subsp. spizizenii in vitro [33]. A recent study also showed that B. subtilis subsp.…”

Section: Discussionmentioning

confidence: 93%

Optimization of antifungal activity by Bacillus subtilis isolate CCASU 2021-4 using Response Surface Methodology

El-Housseiny

Shams

Ghobashi

et al. 2021

Archives of Pharmaceutical Sciences Ain Shams University

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Fungal infections represent an enormous load on the public especially with the development of resistance to the most currently used antifungal drugs in practice. In the present work, a bacterial isolate coded A3 was recovered from soil and was shown to express antagonistic activity against Candida (C.) albicans ATCC 10231. This isolate was identified as Bacillus (B.) subtilis subsp. spizizenii isolate CCASU 2021-4 using 16S ribosomal RNA sequencing. D-optimal design from response surface methodology (RSM) was used to optimize the environmental variables affecting the antifungal activity of the respective isolate. The optimum conditions were a temperature of 30 °C, a pH of 8, and an inoculum size of 5 x 10 7 cfu/mL, resulting in an enhancement in the antifungal activity by 1.2 fold. This is the first report, to the best of our knowledge, on an antifungal activity from B. subtilis subsp. spizizenii culture broth against human fungal pathogens along with its optimization through RSM.

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“…Biological management of this pathogen stands as the best choice considering human health and environmentally friendly nature. Bacterial biological control agents are most commonly used due to their endospore formation which helps to work in a wide range of environments (Priyanka et al, 2018). B. subtilis is extensively employed for plant disease management owing to its effective antimicrobial capabilities (Chandrasekaran et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Identification of secondary metabolites biosynthetic genes, antagonistic activity and potential mechanism of <i>Bacillus subtilis</i> NBAIR-BSWG1 in suppression of <i>Alternaria alternata</i>

RUQIYA,

GIRISHA,

RANGESHWARAN

et al. 2023

JBC

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Alternaria alternata wreaks havoc on fruit and vegetable production globally, threatening food security by causing black leaf spot disease. Bacillus subtilis, a natural inhabitant of soil, is a promising biological control agent for the management of A. alternata. In the present study, the antagonistic potential of B. subtilis NBAIR-BSWG1 was initially confirmed against A. alternata through a dual culture technique with 43.03% inhibition of mycelial growth. Subsequently, we extracted the cell-free extract from the NBAIR-BSWG1 pure culture and assessed its impact on A. alternata through the poison food technique and found mycelial growth inhibition of 85.82%. Identification of secondary metabolites biosynthetic genes using specific PCR markers showed the presence of surfactin genes (sfp, srf AA) with an amplicon size of 675 bp and 201 bp, respectively. Amplification of fengycin (fenB) and iturin (ituD) at 670 bp and 423 bp respectively, by using a specific PCR primer confirms the contribution of fengycin and iturin for the antagonistic potential of NBAIR-BSWG1. This study identifies NBAIR-BSWG1 as an effective bacterial biocontrol agent for control of A. alternata, unlocks the genetic basis of antifungal activity NBAIR-BSWG1, depicts molecular mechanisms involved in biological suppression of A. alternata by NBAIR-BSWG1 paving the way for the development of bioformulations for management of A. alternata.

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Antifungal activity of Bacillus subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight of marigold

Cited by 8 publications

References 11 publications

Strategies for the Management of Leaf Blight Disease Caused by Alternaria alternata in Gloriosa superba (L.)

Strategies for the Management of Leaf Blight Disease Caused by Alternaria alternata in Gloriosa superba (L.)

Optimization of antifungal activity by Bacillus subtilis isolate CCASU 2021-4 using Response Surface Methodology

Identification of secondary metabolites biosynthetic genes, antagonistic activity and potential mechanism of <i>Bacillus subtilis</i> NBAIR-BSWG1 in suppression of <i>Alternaria alternata</i>

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