This attempt was assigned to evaluate the phytochemical constituents, antifungal, antibacterial and cytotoxicity of eleven medicinal plants from Bhandhara area. The phytochemicals such as steroids, anthraquinone, tannins, saponin, alkaloids, phlobatannin, glycosides, quinine and flavonoids were tested qualitatively. Antifungal, antibacterial and cytotoxicity was evaluated with the respective strains in laboratory condition. The total contents of flavonoids were examined quantitatively in eleven plant species and showed maximum (23%) contents from Terminalia chebula (Retz) followed by Vitex nigundo (L). The antibacterial, antifungal and cytotoxicity were conducted. The highest antibacterial potential was revealed from the extracts of Termanalia chebula (Retz) against E. coli, followed by Glycyrrhiza glabra (L) to Pseudomonas pudita. Antifungal activity of eleven plants was tested against the four fungal strains and showed that highest potentials in Termanalia chebula (Retz) while followed by Vitex nigundo (L) and Bacopa mannieri (L). The cytotoxicity was reported on chick embryo cell line against eleven species for their establishment and two species were showed high percent viability fromTerminalia chebula (Retz) (72.2%) followed by Acacia catechu (L.) (65.8%). The viability varies species to species. The phytochemicals are the indicatives of the plant species could be a possible source of obtaining new and effective source of herbal medicine.The antibacterial, antifungal potential activities are better source for further herbal medicine.