Antifungal activity of a prenylated flavonoid from Dalea elegans against Candida albicans biofilms

Peralta, Mariana Andrea; Silva, María Angel da; Ortega, Marı́a Gabriela; Cabrera, José Luis; Paraje, María Gabriela

doi:10.1016/j.phymed.2015.07.003

Cited by 71 publications

(77 citation statements)

References 26 publications

(9 reference statements)

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“…The assay was adjusted to 96-well microtiter plates (Greiner Bio-One, Germany) according to previously published procedure (Arce Miranda et al 2011;Peralta et al 2015). Briefly, plates were pretreated with 50% FBS in SDB at 37 C for 30 min and washed with 10 mM PBS (pH 7.2) twice.…”

Section: Biofilm Formation Assaymentioning

confidence: 99%

“…Isolates classified as strong biofilm producer, were selected to be used in the in vitro antifungal activity test. The amount of formed biofilm was expressed as biofilm biomass unit (BBU), which was arbitrarily defined as 0.1 OD 595 equal to 1 BBU (Arce Miranda et al 2011;Peralta et al 2015).…”

Section: Biofilm Formation Assaymentioning

confidence: 99%

“…After that, both microplates were incubated at 37 C for 48 h. Then, the supernatant was removed in order to measure the production of ROS and RNI, the SOD activity, and the total antioxidant activity of the biological system by FRAP assay. After removing the supernatant, the biofilm formation was quantified and the results were expressed as BBU (0.1 OD 595 nm ¼ 1 BBU) (O'Toole & Kolter, 1998;Arce Miranda et al 2011;Peralta et al 2015). The percentage of reduction was calculated using the following formula:…”

Section: In Vitro Antifungal Activity Assay Over Biofilmsmentioning

confidence: 99%

“…Then, disks were stained with 30 lL of Calcofluor-White (0.05% v/v, Sigma-Aldrich) for 1 min. Calcofluor-White, a carbohydrate-binding fluorescent dye that stains the cell walls of fungi blue , was excited at 355 nm (Peralta et al 2015). Disks were airdried for 15 min in darkness and were placed inverted in 35 mm glass-bottom microwell dishes.…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

“…Reactive oxygen species production ROS were detected by their oxidative action that causes the nitro blue tetrazolium (NBT) reduction to nitroblue diformazan, by following the methodology previously described by us (Paraje et al 2009;Arce Miranda et al 2011;Peralta et al 2015). Since the by-product of this assay is proportional to the ROS generated in biofilms, its absorbance was measured at 540 nm in the same microplate reader used earlier.…”

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

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Reduction ofCandida tropicalisbiofilm by photoactivation of aHeterophyllaea pustulataextract

Marioni

Arce

Cabrera

et al. 2016

Pharmaceutical Biology

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Context: Biofilm formation is an important problem, since this growth mode confers resistance to drugs usually used in therapeutics. Objective: In vitro antifungal activity of extracts obtained from Heterophyllaea pustulata Hook f. (Rubiaceae) were studied against Candida tropicalis biofilms, evaluating the effect of irradiation and the oxidative and nitrosative stresses as possible mechanisms of action. Materials and methods: Hexane, benzene, ethyl acetate and ethanol extracts were evaluated at three concentrations (0.2, 0.1 and 0.05 mg/mL) over mature biofilm, under darkness and irradiation. After 48 h of incubation, biofilm quantitation was performed by the O'Toole and Kolter method. Reactive oxygen species (ROS) was measured by nitro-blue tetrazolium (NBT) reaction and reactive nitrogen intermediates (RNI) by the Griess reagent. Superoxide dismutase activation (SOD, NBT assay) and total antioxidant system (FRAP test) were studied. Results: Only the benzene extract at 0.2 mg/mL reduced the biofilms formation. The slight decrease achieved in darkness (17.06 ± 2.80% reduction) was increased by light action (39.31 ± 3.50% reduction), clearly observing a photostimulation. This great reduction was confirmed by confocal microscopy. In darkness, biofilm reduction was mediated by an increase in RNI, whereas under irradiation, the ROS action was most important. Although no SOD activation was observed, a strong stimulation of the total antioxidant system was detected. HPLC analysis established a high content of several anthraquinones in this extract. Discussion and conclusion: Biofilm reduction by benzene extract was mainly mediated by oxidative stress triggered under light action, confirming a photodynamic sensitization, which could be attributed to its high content of photosensitizing anthraquinones. ARTICLE HISTORY

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Section: Biofilm Formation Assaymentioning

confidence: 99%

Section: Biofilm Formation Assaymentioning

confidence: 99%

Section: In Vitro Antifungal Activity Assay Over Biofilmsmentioning

confidence: 99%

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

Section: Confocal Laser Scanning Microscopy (Clsm)mentioning

confidence: 99%

See 3 more Smart Citations

Reduction ofCandida tropicalisbiofilm by photoactivation of aHeterophyllaea pustulataextract

Marioni

Arce

Cabrera

et al. 2016

Pharmaceutical Biology

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Synthesis of Pterocarpan Derivatives and their Inhibitory Effects against Microbial Growth and Biofilms

et al. 2016

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Pterocarpans are rare isoflavone derivatives that occur in limited Fabaceous plants. The Heck oxyarylation of 2H‐chromenes with o‐iodophenols offered access to several naturally occurring pterocarpans. Among them, medicarpin showed antifungal activity to Fusarium; however, these compounds were nearly water‐insoluble. To overcome this drawback, azamedicarpin HCl salt as a medicarpin mimic was designed and prepared via the Heck azaarylation. Effects of the salt against growth and biofilms of clinically relevant bacteria and fungi were studied. Our findings exemplified that azapterocarpan HCl salt may be beneficial as a potentiated water‐soluble form and valuable for biofilm‐related Candida infections.

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Flavonoids

Ribeiro

Melo

Vilas-Boas

et al. 2023

Natural Secondary Metabolites

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Antifungal activity of a prenylated flavonoid from Dalea elegans against Candida albicans biofilms

Cited by 71 publications

References 26 publications

Reduction ofCandida tropicalisbiofilm by photoactivation of aHeterophyllaea pustulataextract

Reduction ofCandida tropicalisbiofilm by photoactivation of aHeterophyllaea pustulataextract

Synthesis of Pterocarpan Derivatives and their Inhibitory Effects against Microbial Growth and Biofilms

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