Antifungal activity of a novel 3-Alkylpyridine analog derived from Marine sponge alkaloids

Andrade, Jéssica Tauany; Lima, William Gustavo; Barbosa, Camila de Souza; Gonçalves, Alessandra Mirtes Marques Neves; Silva, Mayra K P; Morais, Fernanda Barbara; Palumbo, Juliana Maria Campos; Viana, Gustavo Henrique Ribeiro; Ferreira, Jaqueline Maria Siqueira

doi:10.1590/0001-3765202120200944

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“…Alkyl Pyridine Alkaloids (3-APAs) are generally produced by sponges of the genus Haliclona, [17][18][19][20][21] Amphimedon, [22][23][24] Topsentia, 25 Neopetrosia, 26 Xestospongia, 27 and Cribrochalina. 28 They have also been reported to have pharmacological potentials, such as cytotoxic, 17 antifungal, 29,30 anticancer, 31 and antimicrobial activities 32 . © 2022 the authors.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Evaluation of Anti-Mycobacterial Activity of Alkaloid Compounds from Marine Invertebrate Sponge Haliclona sp.

2022

TJNPR

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Materials and Methods General Experimental ProceduresOn a JEOL spectrometer, CD3OD's 1 H and 13 C NMR spectra were captured at 400 MHz for 1 H and 100 MHz for 13 C (H 3.31, C 49.0), after which HRFAB mass spectrometry was carried out using JEOL JMS-MS 700. The L-6200 system was used for preparative HPLC (Hitachi Ltd.), while UV was performed on Hitachi U-3310 UV. Animal MaterialThe marine invertebrate sponge was discovered in 2013 by scuba diving in the sea off Manado, North Sulawesi, Indonesia. Dr. K. Ogawa (Z. Nakai Laboratory Japan) classified the samples (2013-147) as Haliclona sp. and they were subsequently preserved at the Laboratory of Natural Product, Tohoku Medical and Pharmaceutical University-Japan Extraction and IsolationThe marine sponge sample (619.9 g) was sliced into small pieces, steeped in 70% ethanol three times, and partitioned with 200 mL of EtOAc to obtain 2.6 g of extract. The ethyl acetate extract was then separated into 7 fractions using a chromatographic column with ODS stationary phase (100 g) as well as MeOH and H2O mobile phases. To obtain compound 3, 190.5 mg of fraction 7 was purified using HPLC with 1.8 mg of MeOH:H2O solvent (65:35 v/v). HPLC was also used to separate 1.8 mg of 4 from 201.8 mg of fraction 6 using MeOH:H2O (65:35 v/v). Subsequently, 1.8 mg of 8 and 1.8 mg of 9 were isolated from 219.2 mg fraction 5 using the same solvent. The HPLC column used was the PEGASIL ODS. Anti-Mycobacterial AssayThe disc diffusion method was used to conduct the anti-mycobacterial assay. 12,13 M. smegmatis strain NBRC 3207 was earned from laboratory culture stock stored at -80 o C. A Middlebrook 7H9 broth medium containing 0.05% polysorbate 80 (BD), 0.5% glycerol, and 10% Middlebook OADC (BD) was used to cultivate the test bacteria for two days at 37°C. 1 mL of inoculum was poured into 100 mL Middlebook 7H9 agar medium at 40°C. Subsequently, isolated compounds in MeOH were placed on paper discs and then evaporated to remove

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Section: Resultsmentioning

confidence: 99%

Isolation and Evaluation of Anti-Mycobacterial Activity of Alkaloid Compounds from Marine Invertebrate Sponge Haliclona sp.

2022

TJNPR

View full text Add to dashboard Cite

show abstract

Antifungal activity of a novel 3-Alkylpyridine analog derived from Marine sponge alkaloids

Cited by 1 publication

References 38 publications

Isolation and Evaluation of Anti-Mycobacterial Activity of Alkaloid Compounds from Marine Invertebrate Sponge Haliclona sp.

Isolation and Evaluation of Anti-Mycobacterial Activity of Alkaloid Compounds from Marine Invertebrate Sponge Haliclona sp.

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