Antifungal activity of 9-hydroxy-folianin and sucrose octaacetate from the seeds of Annona cornifolia A. St. -Hil. (Annonaceae)

Different susceptibilities of Leishmania spp. promastigotes to the Annona muricata acetogenins annonacinone and corossolone, and the Platymiscium floribundum coumarin scoparone

“…Acetogenins are widely studied due to its anticancer (Chen et al, 2012b; D’Ambrosio et al, 2011; Tantithanaporn et al, 2011), antimicrobial (Parellada et al, 2011; Lima et al, 2011) and antiprotozoal (Boyom et al, 2009; Camacho et al, 2003; Grandic et al, 2004) properties. Vila-Nova et al (2011) reported the effect of annonacinone and corossolone isolated from A. muricata against L. chagasi promastigote and amastigote forms, and analyzed the toxicity against murine macrophages cells.…”

“…The Annonaceae family is a great producer of acetogenins that has shown promising results in the search for new drugs against various protozoa, revealing good potential as a source of agents for the treatment of leishmaniasis (Rocha et al, 2005). Annona muricata is a well-known plant in Brazil, the compounds there are most found in this plant are acetogenins and alkaloids (Vila-Nova et al, 2011; Tempone et al, 2005), and has antiprotozoal, antioxidant and anticancer properties (Rondon et al, 2011; Boyom et al, 2009; Lima et al, 2011; Chen et al, 2012a). Platymiscium floribundum is a regional plant in Ceará state used by the population as anti-inflammatory and the isoflavonoids isolated from this plant have demonstrated activity against cancer cells (Militão et al, 2006; Falcao et al, 2005), and the crude extract demonstrated antifungal and acetylcholinesterase activity (Cardoso-Lopes et al, 2008), pterocarpans isolated from the heartwood demonstrated antimitotic effect on sea urchin eggs (Militão et al, 2005), but a few of studies have been developed in the search of others chemotherapeutic values as well as isolating others compounds from this plant.…”

Influence of Different Media, Incubation Times, and Temperatures for Determining the MICs of Seven Antifungal Agents against Paracoccidioides brasiliensis by Microdilution

“…First, a previous testing assay with three different inoculum concentrations (10 3 , 10 4 , and 10 5 cells/ml) in RPMI 1640 medium was performed with 13 isolates. The use of 10 3 cells/ml is suggested by the NCCLS M27-A2 protocol, though concentrations of 10 4 and 10 5 cells/ml were used in previous P. brasiliensis MIC assay studies (15,16,18,20,21,33). Our results, which are in agreement with the results of these authors, indicated that the obtained growth and MIC readings were easier to perform when a concentration of 10 5 cells/ml was used (data not shown), and this is the inoculum size in the proposed protocol (Table 5).…”

“…These studies have generally used modified versions of the NCCLS M27-A2 protocols or a Shadomy modified protocol (10). These modifications include differences in inoculum preparation, media used in MIC assays, incubation periods, and experimental temperatures (5,6,(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). However, standardization of the methods used for in vitro susceptibility testing is important to facilitate the establishment of interpretative breakpoints and quality control parameters.…”

Exploring genetic biodiversity: secondary metabolites from Neotropical Annonaceae as a potential source of new pesticides

“…, antinociceptive and anti-inflammatory activities(SOUZA et al, 2008), of crude ethanolic extracts and/or the isoquinoline alkaloid-rich fraction from D. lanceolata leaves have been reported in the literature and corroborate the potential observed in this study.To better elucidate the potential protectant effect on grains, the antifungal and antiaflatoxigenic activities of the most promising extracts (regarding the action on S. zeamais) were evaluated using the A. flavus isolate. Although the fungal toxicity of the crude extracts and acetogenins isolated from the genus Annona species were reported previously for a number of plant species (DANG et al, 2011) and human pathogens(AHMAD et al, 2003;LIMA et al, 2011), the extracts evaluated did not exert pronounced effects on A. flavus. Hypothetically, the lack of fungicidal effect could be because of evolutionary selection in A. flavus species adapted to coexist with these secondary metabolites.…”