Antifungal Activity of 2-Hydroxy-4-Methoxybenzaldehyde Isolated From Decalepis Hamiltonii (Wight & Arn.) on Seed-Borne Fungi Causing Biodeterioration of Paddy

Abstract

Abstract:In vitro antifungal activity assay of different concentrations of 2-hydroxy-4-methoxybenzaldehyde isolated from Decalepis hamiltonii against six important seed-borne fungal pathogens viz., Alternaria alternata, Drechslera tetramera, Fusarium oxysporum, F. proliferatum, Pyricularia oryzae and Trichoconis padwickii isolated from paddy seeds revealed that, the compound 2-hydroxy-4-methoxybenzaldehyde showed significant antifungal activity. Among the fungi tested, F. proliferatum showed highest inhibitory activity, whereas P. oryzae showed least inhibitory activity. The minimal inhibitory concentration (MIC) varied between 350 µg/ml and 650 µg/ml depending on the fungal species. Comparative evaluation of the active compound with the synthetic fungicide thiram at recommended dosage revealed that, the antifungal activity of the active compound obtained from the plant was almost equivalent.Evaluation for nutritional parameters and dry matter losses (DML) revealed that, total carbohydrates, water soluble proteins, lipids and dry matter losses were significantly confined in 2-hydroxy-4-methoxybenzaldehyde treated paddy seeds compared with control seeds. This plant being an edible one can be exploited in the management of seed-borne pathogenic fungi and in the prevention of biodeterioration of grains and mycotoxin production during storage in an eco-friendly way.

Efficacy of seed hydropriming with phytoextracts on plant growth promotion and antifungal activity in maize

“…The plant extracts of P. corylifolia, T. asiatica and D. hamiltonii offered significant antifungal activity against A. flavus and F. verticillioides, while the extracts of V. negundo showed inhibition only against F. verticillioides ( Table 2). The above-mentioned plant species are reported to be endowed with antimicrobial compounds such as flindersine in T. asiatica (Duraipandiyan & Ignacimuthu 2009), 2H-Furo [2, 3-H]-1-benzopyran-2-one in P. corylifolia (Kiran et al 2011b), antifungal flavonoid glycoside in V. negundo (Zheng et al 2014;Sathiamoorthy et al 2007) and 2-Hydroxy-4-Methoxybenzaldehyde in D. hamiltonii (Mohana et al 2009).…”

Antifumonisin Efficacy of 2-Hydroxy-4-Methoxybenzaldehyde Isolated fromDecalepis hamiltonii

“…The amount of FB 1 was estimated qualitatively and quantitatively using spectrophotodensitometer (Biorad, Universal Hood II 720BR/02170) at 600 nm with different concentrations of standard FB 1 . The mycelial mat of F. verticillioides obtained after the filtration of the SDB media was used for the estimation of MDW losses following the procedure of Shukla et al [23] The in vivo efficacies of HMB on FB 1 production, seed-borne mycoflora and seedling vigor in maize seeds were determined following the standard procedures [7,24] with some modifications. Briefly, freshly harvested maize samples were collected, surface sterilized under UV and the water activity (a w ) was adjusted to 0.95 by adding sterile distilled water.…”

Phytochemical Analysis of Leucas aspera(Willd.)Link. from Dibrugarh

“…Different phyto-chemicals have been isolated from the plant by various workers (Mangathayaru et al, 2006;Rahman et al, 2007;Ilango et al, 2008;Prajapati et al, 2010;Srinivasan et al, 2011;Morshed et al, 2011;Shiney Ramya et al, 2012). Some other experiments on the plant were carried out by, Prasad and Kumar (2012); Unni et al, (2009); Mominul-Islam and Keto-Noguchi, (2012); Mohana et al, (2008).…”