Antifungal Activity in Transgenic Peanut (<i>Arachis hypogaea</i>L.) Conferred by a Nonheme Chloroperoxidase Gene

Niu, Chen; Akasaka-Kennedy, Yoko; Faustinelli, Paola C.; Joshi, M. C.; Rajasekaran, Kanniah; Yang, Hong-Chang; Chu, Ye; Cary, Jeffrey W.; Ozias‐Akins, Peggy

doi:10.3146/ps08-020.1

Cited by 22 publications

(11 citation statements)

References 35 publications

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“…A nonheme chloroperoxidase gene (cpo-p) from Pseudomonas pyrrocinia, which converts reactive oxygen species, such as hydrogen peroxide into antimicrobial peracetic acid and hypohalites (Jacks et al, 2000;Van Pée, 1996), was introduced into peanut and cotton. In vitro bioassays using crude protein extracts from transgenic peanut and cotton plants and their progenies showed inhibition of A. flavus hyphal growth (Niu et al, 2009;Rajasekaran et al, 2008b). We have provided below examples of antifungal proteins and peptides showing efficacy in controlling A. flavus in maize.…”

Section: Enhancement Of Host Resistance Expression Of Antifungal Protmentioning

confidence: 96%

Discovery and confirmation of genes/proteins associated with maize aflatoxin resistance

Chen

Rajasekaran

Brown

et al. 2015

WMJ

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Maize (Zea mays L.) is one of the major crops susceptible to Aspergillus flavus infection and subsequent aflatoxin contamination. Many earlier studies indicated the roles of kernel proteins, especially constitutively expressed proteins, in maize resistance to A. flavus infection and aflatoxin production. In this review, we examined the past and current efforts in identifying maize genes and proteins from kernel, rachis, and silk tissues that may play an important role in resistance to A. flavus infection and aflatoxin contamination, as well as the efforts in determining the importance or involvement of them in maize resistance through biochemical, molecular and genetics studies. Through these studies, we gained a better understanding of host resistance mechanism: resistant lines appear to either express some stress-related and antifungal proteins at higher levels in endosperm, embryo, rachis and silk tissues before A. flavus infection or induce the expression of these proteins much faster compared to susceptible maize lines. In addition, we summarised several recent efforts in enhancing maize resistance to aflatoxin contamination using native genes from maize or heterologous and synthetic genes from other sources as well as from A. flavus. These efforts to either suppress A. flavus growth or aflatoxin production, have all shown some promising preliminary success. For example, maize plants transformed with an α-amylase inhibitor protein from Lablab purpurea showed reduced aflatoxin levels by 56% in kernel screening assays. The antifungal potentials of transgenic maize plants expressing synthetic lytic peptides, such as cecropin-based D4E1 or tachyplesin-based AGM peptides with demonstrated antiflavus activity (IC 50 = 2.5 to 10 μM), are yet to be assayed. Further investigation in these areas may provide a more cost-effective alternative to biocontrol in managing aflatoxin contamination in maize and other susceptible crops.

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Section: Enhancement Of Host Resistance Expression Of Antifungal Protmentioning

confidence: 96%

Discovery and confirmation of genes/proteins associated with maize aflatoxin resistance

Chen

Rajasekaran

Brown

et al. 2015

WMJ

Self Cite

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“…Significantly reduced lesion size was recorded in transgenic plants expressing a barley oxalate oxidase gene compared to the controls, which means that oxalate oxidase can confer enhanced resistance to Sclerotinia blight in peanuts (Livingstone et al, 2005). A non-heme chloroperoxidase gene (cpop) from Pseudomonas pyrrocinia, a growth inhibitor of mycotoxinproducing fungi, introduced into peanuts resulted in transgenic plants that showed inhibition of A. flavus hyphal growth and reduced aflatoxin contamination of peanut seeds (Niu et al, 2009).…”

Section: Transgenic Approach For Biotic Stress Managementmentioning

confidence: 99%

Advancements in molecular marker development and their applications in the management of biotic stresses in peanuts

et al. 2015

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“…Rajasekaran et al (2000) reported that the expression of a chloroperoxidase gene from Pseudomonas pyrrocinia (cpo-p) in transgenic tobacco resulted in significant inhibition of Aspergillus flavus hyphal growth and reduced leaf anthracnose lesions caused by Colletotrichum destructivum. Recently, Niu et al (2009) also demonstrated the successful insertion and expression of the cpo-p gene derived from Pseudomonas pyrrocinia into the groundnut Arachis hypogaea. The developed transgenic groundnut plants showed inhibition of Aspergillus flavus hyphal growth, which resulted in the reduction of aflatoxin contamination in peanut seeds.…”

Section: Mycotoxinsmentioning

confidence: 99%

Genes of Microorganisms: Paving Way to Tailor Next Generation Fungal Disease Resistant Crop Plants

et al. 2011

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The automation of sequencing technologies, flooding in the knowledge of plant-pathogen interactions and advancements in bioinformatics provide tools leading to better knowledge not only of the genome of plant pathogens or microorganism beneficial to plants but also of ways of incorporating genes from microbes into plants as microbial-derived resistance. The identification of various microorganism genes playing key role during pathogensis and the dissection of the signal transduction components of the hypersensitive response and systemic acquired resistance pathways have greatly increased the diversity of options available for tailoring fungus resistant crops. The genetically engineered plants carrying these genes showed spontaneous activation of different defense mechanisms, leading the plant in an elevated state of defense. This 'defense mode' greatly enhances the plant's ability to quickly react to a pathogen invasion and more successfully overcome the infection. The aim of this review is to highlight the dynamic use of genes of microorganisms in enhancing crop tolernace towards fungal intruders by examining the most relevant research in this field.

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Antifungal Activity in Transgenic Peanut (Arachis hypogaeaL.) Conferred by a Nonheme Chloroperoxidase Gene

Cited by 22 publications

References 35 publications

Discovery and confirmation of genes/proteins associated with maize aflatoxin resistance

Discovery and confirmation of genes/proteins associated with maize aflatoxin resistance

Advancements in molecular marker development and their applications in the management of biotic stresses in peanuts

Genes of Microorganisms: Paving Way to Tailor Next Generation Fungal Disease Resistant Crop Plants

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