Antifungal activity and acute toxicity of stem bark extracts of <i>Drypetes Gossweileri</i> S. Moore-Euphorbiaceae from Cameroon

Abstract

Drypetes gossweilleri S. Moore is a plant used in traditional medicine in Cameroon. The antifungal properties of its stem-bark crude extract and fractions DG(1), DG(2), DG(3), DG(4), DG(5), DG(6), DG(7), DG(8) and DG(9) were assayed by agar and broth dilution methods on solid and liquid media against C. Krusei, C. albicans, C. glabrata, T. mentagerophytes, M. langeroinii, M. gypeum, M. audouini, T. rubrum, T. soudanense, T. terrestre, A. flavus and A. niger. The results revealed a substantial antifungal effect with minimal inhibitory concentrations ranging respectively from 24.11µg/ml to 1562µg/ml for yeasts and from 3125µg/ml to 12500µg/ml for filamentous fungi. Among the fractions, fraction DG4 exerted the highest antifungal activity. Moreover, no toxic effect was noticed in male and female albinos Wistar rats treated per os with the crude stem bark's extract of Drypetes gossweileri at a dose up to 12g/kg of body weight. The phytochemical screening of the crude extract and fractions showed the presence of alkaloids, phenols, flavonoids, saponins, anthocyanines, anthraquinones, sterols, lipids and essential oils. Therefore, Drypetes gossweileri may be safe as phytomedecine for the treatment of fungal infections.

In vitro evaluation of the wound healing activity of Drypetes klainei stem bark extracts

“…S1 b and S2). A preliminary phytochemical screening on the active extracts highlighted the presence of polyphenols, flavonoids and triterpenoids, in accordance with the chemical composition reported for other related species (Ngouana et al, 2011;Ng'ang'a et al, 2011¸Wandji et al 2003. The comparison between the HPLC fingerprint of the traditional preparation (WE) and the DME, allowed to identify some common zones in the chromatogram profiles (Fig.…”

Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao

“…Fraction FD displayed no antifungal activity. The differential distribution of bioactivity among the fractions might be explained by the proportions of different bioactive components of each fraction, acting either in a synergetic or in potentiating way [44]. Since FA showed the strongest inhibition of mycelial growth, we chose it for further analytical studies.…”

Phytochemistry and pharmacology of the genus Drypetes: A review

“…Morphological examination of various organs and fluctuations in their relative weights revealed no differences between control groups (treated with distilled water or maize oil) and treated rats (Ngouana et al, 2010). Moreover, no toxic effect was noticed in male and female albino Wistar rats treated per os with the crude stem bark extract at a dose up to 12 g/kg of body weight (Ngouana et al, 2011).…”

“…The antifungal properties of the DCM-MeOH ( (Ngouana, 2011). From the MeOH extract of the stem bark, the isolated -amyrone 43 and N-β-glucopyranosyl-p-hydroxyphenylacetamide 117, exhibiting activity against C. albicans with MIC 16 µg/mL and 8.0 μg/mL, respectively (Ata et al, 2011).…”

“…This is why the interdisciplinary approach (biologist, phytochemists and pharmacologist) should be followed, to conduct a comprehensive research work on any species. Dalziel and Hutchinson, 1937;Bouquet and Debray, 1974;Wansi et al, 2006Wansi et al, , 2007Chen et al, 2014 Drypetes Drypetes gossweileri The bark is used in Western Africa for the treatment of pain Extract displayed significant analgesic activity in animal models, and was not toxic in animal model as well Bouquet, 1969;Burkill, 1985;Muganza et al, 2012;Ngouana et al, 2010Ngouana et al, , 2011Bomba et al, 2013 Drypetes natalensis Unspecified parts of the plant are used for the treatment of malaria in Tanzania…”