Antifilarial activity in vitro and in vivo of some flavonoids tested against Brugia malayi

Lakshmi, Vijai; Joseph, Sujith; Srivastava, S. C.; Verma, Shailendra Prasad; Sahoo, Malaya K.; Dube, V.; Mishra, Sunil Kumar; Murthy, P. K.

doi:10.1016/j.actatropica.2010.06.006

Cited by 68 publications

(47 citation statements)

References 46 publications

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“…After vascular injury, the abnormal proliferation of VSMCs has long been considered as a critical etiological factor of atherosclerosis and restenosis and these vascular diseases are the common causes of severe cardiovascular disease (Ross, 1994(Ross, , 1995 Compound 1, (2S)-naringenin, has been reported a beneficial effects, such as anticancer, anti-filarial activity, and improvement of insulin sensitivity and increase of glucose uptake (Frydoonfar et al, 2003;Kannappan and Anuradha, 2010;Lakshmi et al, 2010;Zygmunt et al, 2010). It also reduced neointimal hyperplasia following reconstruction with interpositional vein graft in vivo (Cayci et al, 2010).…”

Section: Resultsmentioning

confidence: 96%

A potential inhibitor of rat aortic vascular smooth muscle cell proliferation from the pollen of Typha angustata

et al. 2010

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By various chromatographic methods, three flavonoids, (2S)-naringenin (1), isorhamnetin 3-O-(2-O-α-L-rhamnopyranosyl) β-D-glucopyranoside (2), typhaneoside (3), and two sterol glycosides, β-sitosterol-3-O-(6-octadecanoyl) β-D-glucopyranoside (4) and β-sitosterol-3-O-(6-octadeca-9Z,12Z-dienoyl) β-D-glucopyranoside (5), were isolated from the pollen of Typha angustata. Their structures were determined on the basis of spectroscopic analyses. The flavonoids (1-3) were evaluated for their effects on the viability and proliferation of rat aortic smooth muscle cells. (2S)-naringenin (1) significantly inhibited cell proliferation in a dose-dependent manner without cytotoxic at concentrations of 30, and 50 μM; it reduced the number of cells following PDGF-BB treatment to 1.83 ± 0.30 × 10(4) and 2.20 ± 0.60 × 10(4) cells/well, respectively. These findings suggest that (2S)-naringenin has antiproliferative effects on aortic smooth muscle cells.

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Section: Resultsmentioning

confidence: 96%

A potential inhibitor of rat aortic vascular smooth muscle cell proliferation from the pollen of Typha angustata

et al. 2010

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“…Results showed that naringenin and fl avone have activities against Brugia malayi, which may provide a lead for design and development of new antifilarial. (34) In another study, effect of naringenin on Salmonella Typhimurium LT2 was investigated. Naringenin was found to be active against this microorganism with specifi cally repressed 24 genes in the Salmonella pathogenicity island 1 and down-regulated 17 genes involved in fl agellar and motility.…”

Section: Pharmacological Activities Of Naringeninmentioning

confidence: 99%

A Review on Pharmacological and Analytical Aspects of Naringenin

Patel

Singh

Patel

2014

Chin. J. Integr. Med.

180

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Flavonoids are a widely distributed group of phytochemicals having benzo-pyrone nucleus, and more than 4,000 different flavonoids have been described and categorized into flavonols, flavones, flavanones, isoflavones, catechins and anthocyanidins. Flavonoids occurs naturally in fruits, vegetables, nuts, and beverages such as coffee, tea, and red wine, as well as in medical herbs. Flavonoids are responsible for the different colors of plant parts and are important constituents of the human diet. Flavanoids have different pharmacological activities, such as antioxidant, anti-allergic, antibacterial, anti-inflammatory, antimutagenic and anticancer activity. Naringenin belongs to the flavanones and is mainly found in fruits (grapefruit and oranges) and vegetables. Pharmacologically, it has anticancer, antimutagenic, anti-inflammatory, antioxidant, antiproliferative and antiatherogenic activities. Naringenin is used for the treatments of osteoporosis, cancer and cardiovascular diseases, and showed lipid-lowering and insulin-like properties. In the present review, detailed pharmacological and analytical aspects of naringenin have been presented, which revealed the impressive pharmacological profile and the possible usefulness in the treatment of different types of diseases in the future. The information provided in this communication will act as an important source for development of effective medicines for the treatment of various disorders.

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“…Determination of IC 50 and CC 50 IC 50 (the concentration at which the parasite motility was inhibited by 50%) and CC 50 (Cytotoxic concentration 50) of extract, fraction and DEC were determined as described elsewhere (Lakshmi et al 2010).…”

Section: In Vitromentioning

confidence: 99%

Antifilarial efficacy of Hibiscus sabdariffa on lymphatic filarial parasite Brugia malayi

Dube

Kushwaha

et al. 2010

Med Chem Res

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Human lymphatic filariasis, a vector borne debilitating disease is widely prevalent in tropical and subtropical regions of the world and imposes a significant public health and economic burden. Despite global efforts towards elimination of the disease by treatment with existing drugs, diethylcarbamazine (DEC), ivermectin and albendazole and their combinations, the disease is continuing. There is therefore a need for more effective therapeutic alternatives. In the present study, we evaluated antifilarial activity of ethanolic extract of leaves of plant Hibiscus sabdariffa by studying in vitro motility and MTT reduction assays employing microfilariae (mf) and adult worms of human lymphatic filarial parasite, Brugia malayi in B. malayi-jird and B. malayi-Mastomys coucha models. The leaf-extract affected both adult worms and microfilariae in vitro. Out of four fractions, n-butanol insoluble fraction killed mf at 250 lg/ml conc in vitro. Extract and its n-butanol insoluble fraction showed reasonably high selectivity index with respect to motility of mf (10 to [20). In B. malayi-jird model the leaf-extract at 500 mg/kg, p.o. 9 5 days produced about 30% macrofilaricidal efficacy and 42% sterilization of female worms. In B. malayi/M. coucha the extract (1 g/kg, p.o. 9 5 days) exerted 57.0% macrofilaricidal with 64% sterilizing effect on female worms. HPLC profile showed nine components in the n-butanol insoluble fraction. The components eluting at 26.7 and 28.4 min seemed to be the major components present in the fraction. NMR spectra (DQF-COSY, gHSQC, and gHMBC) of the fraction also displayed characteristic peaks of anthocyanin-glycosides. Thus, it is concluded that the plant may provide valuable leads for developing potent macro-and or microfilaricidal agent(s). This is the first ever report on the antifilarial efficacy of H. sabdariffa.

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Antifilarial activity in vitro and in vivo of some flavonoids tested against Brugia malayi

Cited by 68 publications

References 46 publications

A potential inhibitor of rat aortic vascular smooth muscle cell proliferation from the pollen of Typha angustata

A potential inhibitor of rat aortic vascular smooth muscle cell proliferation from the pollen of Typha angustata

A Review on Pharmacological and Analytical Aspects of Naringenin

Antifilarial efficacy of Hibiscus sabdariffa on lymphatic filarial parasite Brugia malayi

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